Accurately Determining Esterase Activity via the Isosbestic Point of p-Nitrophenol
Esterase is an important enzyme for ester hydrolysis or synthesis. Its activity, however, has not been accurately ascertained due to a lack of accurate protocols. In this study, the isosbestic point of p-nitrophenol was found and used as the marker for its activity. The methodology avoided decomposi...
Main Authors: | , , , |
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Format: | Article |
Language: | English |
Published: |
North Carolina State University
2016-10-01
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Series: | BioResources |
Subjects: | |
Online Access: | http://ojs.cnr.ncsu.edu/index.php/BioRes/article/view/BioRes_11_4_10099_Peng_Esterase_Activity_Nitrophenol_9 |