Rapid whole brain imaging of neural activity in freely behaving larval zebrafish (Danio rerio)

The internal brain dynamics that link sensation and action are arguably better studied during natural animal behaviors. Here, we report on a novel volume imaging and 3D tracking technique that monitors whole brain neural activity in freely swimming larval zebrafish (Danio rerio). We demonstrated the...

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Main Authors:	Lin Cong, Zeguan Wang, Yuming Chai, Wei Hang, Chunfeng Shang, Wenbin Yang, Lu Bai, Jiulin Du, Kai Wang, Quan Wen
Format:	Article
Language:	English
Published:	eLife Sciences Publications Ltd 2017-09-01
Series:	eLife
Subjects:	freely behaving larval zebrafish whole brain Imaging 3D tracking system light field microscope prey capture behavior
Online Access:	https://elifesciences.org/articles/28158

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record_format	Article
spelling	doaj-94255e78aeae41438d218b01d1e8ca712021-05-05T13:49:12ZengeLife Sciences Publications LtdeLife2050-084X2017-09-01610.7554/eLife.28158Rapid whole brain imaging of neural activity in freely behaving larval zebrafish (Danio rerio)Lin Cong0Zeguan Wang1Yuming Chai2Wei Hang3Chunfeng Shang4Wenbin Yang5Lu Bai6Jiulin Du7Kai Wang8https://orcid.org/0000-0001-7858-944XQuan Wen9https://orcid.org/0000-0003-0268-8403Institute of Neuroscience, State Key Laboratory of Neuroscience, CAS Center for Excellence in Brain Science and Intelligence Technology, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, Shanghai, ChinaCenter for Integrative Imaging, Hefei National Laboratory for Physical Sciences at Microscale, CAS Center for Excellence in Brain Science and Intelligence Technology, School of Life Sciences, University of Science and Technology of China, Hefei, ChinaCenter for Integrative Imaging, Hefei National Laboratory for Physical Sciences at Microscale, CAS Center for Excellence in Brain Science and Intelligence Technology, School of Life Sciences, University of Science and Technology of China, Hefei, ChinaInstitute of Neuroscience, State Key Laboratory of Neuroscience, CAS Center for Excellence in Brain Science and Intelligence Technology, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, Shanghai, ChinaInstitute of Neuroscience, State Key Laboratory of Neuroscience, CAS Center for Excellence in Brain Science and Intelligence Technology, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, Shanghai, ChinaCenter for Integrative Imaging, Hefei National Laboratory for Physical Sciences at Microscale, CAS Center for Excellence in Brain Science and Intelligence Technology, School of Life Sciences, University of Science and Technology of China, Hefei, ChinaInstitute of Neuroscience, State Key Laboratory of Neuroscience, CAS Center for Excellence in Brain Science and Intelligence Technology, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, Shanghai, China; University of Chinese Academy of Sciences, Beijing, ChinaInstitute of Neuroscience, State Key Laboratory of Neuroscience, CAS Center for Excellence in Brain Science and Intelligence Technology, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, Shanghai, China; University of Chinese Academy of Sciences, Beijing, ChinaInstitute of Neuroscience, State Key Laboratory of Neuroscience, CAS Center for Excellence in Brain Science and Intelligence Technology, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, Shanghai, China; University of Chinese Academy of Sciences, Beijing, ChinaCenter for Integrative Imaging, Hefei National Laboratory for Physical Sciences at Microscale, CAS Center for Excellence in Brain Science and Intelligence Technology, School of Life Sciences, University of Science and Technology of China, Hefei, ChinaThe internal brain dynamics that link sensation and action are arguably better studied during natural animal behaviors. Here, we report on a novel volume imaging and 3D tracking technique that monitors whole brain neural activity in freely swimming larval zebrafish (Danio rerio). We demonstrated the capability of our system through functional imaging of neural activity during visually evoked and prey capture behaviors in larval zebrafish.https://elifesciences.org/articles/28158freely behaving larval zebrafishwhole brain Imaging3D tracking systemlight field microscopeprey capture behavior
collection	DOAJ
language	English
format	Article
sources	DOAJ
author	Lin Cong Zeguan Wang Yuming Chai Wei Hang Chunfeng Shang Wenbin Yang Lu Bai Jiulin Du Kai Wang Quan Wen
spellingShingle	Lin Cong Zeguan Wang Yuming Chai Wei Hang Chunfeng Shang Wenbin Yang Lu Bai Jiulin Du Kai Wang Quan Wen Rapid whole brain imaging of neural activity in freely behaving larval zebrafish (Danio rerio) eLife freely behaving larval zebrafish whole brain Imaging 3D tracking system light field microscope prey capture behavior
author_facet	Lin Cong Zeguan Wang Yuming Chai Wei Hang Chunfeng Shang Wenbin Yang Lu Bai Jiulin Du Kai Wang Quan Wen
author_sort	Lin Cong
title	Rapid whole brain imaging of neural activity in freely behaving larval zebrafish (Danio rerio)
title_short	Rapid whole brain imaging of neural activity in freely behaving larval zebrafish (Danio rerio)
title_full	Rapid whole brain imaging of neural activity in freely behaving larval zebrafish (Danio rerio)
title_fullStr	Rapid whole brain imaging of neural activity in freely behaving larval zebrafish (Danio rerio)
title_full_unstemmed	Rapid whole brain imaging of neural activity in freely behaving larval zebrafish (Danio rerio)
title_sort	rapid whole brain imaging of neural activity in freely behaving larval zebrafish (danio rerio)
publisher	eLife Sciences Publications Ltd
series	eLife
issn	2050-084X
publishDate	2017-09-01
description	The internal brain dynamics that link sensation and action are arguably better studied during natural animal behaviors. Here, we report on a novel volume imaging and 3D tracking technique that monitors whole brain neural activity in freely swimming larval zebrafish (Danio rerio). We demonstrated the capability of our system through functional imaging of neural activity during visually evoked and prey capture behaviors in larval zebrafish.
topic	freely behaving larval zebrafish whole brain Imaging 3D tracking system light field microscope prey capture behavior
url	https://elifesciences.org/articles/28158
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Rapid whole brain imaging of neural activity in freely behaving larval zebrafish (Danio rerio)

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