Rapid whole brain imaging of neural activity in freely behaving larval zebrafish (Danio rerio)

• Main Authors: Lin Cong, Zeguan Wang, Yuming Chai, Wei Hang, Chunfeng Shang, Wenbin Yang, Lu Bai, Jiulin Du, Kai Wang, Quan Wen
• Format: Article
• Language: English
• Published: eLife Sciences Publications Ltd 2017-09-01
• Series: eLife
• Subjects: freely behaving larval zebrafish; whole brain Imaging; 3D tracking system; light field microscope; prey capture behavior
• Online Access: https://elifesciences.org/articles/28158
• ISSN: 2050-084X

The internal brain dynamics that link sensation and action are arguably better studied during natural animal behaviors. Here, we report on a novel volume imaging and 3D tracking technique that monitors whole brain neural activity in freely swimming larval zebrafish (Danio rerio). We demonstrated the capability of our system through functional imaging of neural activity during visually evoked and prey capture behaviors in larval zebrafish.