SMART amplification combined with cDNA size fractionation in order to obtain large full-length clones

SMART amplification combined with cDNA size fractionation in order to obtain large full-length clones

<p>Abstract</p> <p>Background</p> <p>cDNA libraries are widely used to identify genes and splice variants, and as a physical resource for full-length clones. Conventionally-generated cDNA libraries contain a high percentage of 5'-truncated clones. Current library c...

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Bibliographic Details
Main Authors: Poustka Annemarie, Schupp Ingo, Wellenreuther Ruth, Wiemann Stefan
Format: Article
Language:English
Published: BMC 2004-06-01
Series:BMC Genomics
Online Access:http://www.biomedcentral.com/1471-2164/5/36

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