ELECTROPORATION OF CONFLUENT HM-1 ES CELLS LEADS TO HIGHER AMOUNT COLONIES

ELECTROPORATION OF CONFLUENT HM-1 ES CELLS LEADS TO HIGHER AMOUNT COLONIES

Electroporation is used to induce homologous recombination in the genome of the murine ES(embryonic stem) cells. Routinelly subconfluent ES cells are recommended to be used in suchexperiments. Electroporation of immunoglobulin specific targeting vectors with different length ofhomology leads to redu...

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Bibliographic Details
Main Authors: BENCSIK A. ALENA, BENCSIK I., STANCULET JANA, BURA M.
Format: Article
Language:English
Published: Agroprint Timisoara 2008-01-01
Series:Scientific Papers Animal Science and Biotechnologies
Subjects:
HM-1
embryonic stem cells
colony number
confluent
subconfluent
selection
Online Access:http://usab-tm.ro/fileadmin/fzb/PDF%202008/Volumul%201/Biotechnologies/bencsik_a%201.pdf
Description
Summary:Electroporation is used to induce homologous recombination in the genome of the murine ES(embryonic stem) cells. Routinelly subconfluent ES cells are recommended to be used in suchexperiments. Electroporation of immunoglobulin specific targeting vectors with different length ofhomology leads to reduced number of selected colonies. The enrichment of double selected colonies ishigh and thus the amount of HM-1 ES cell colonies for the analysis is very low. Here we show that theelectroporation of confluent HM-1 ES cells leads to an increased amount of simple and doubleselected colonies.
ISSN:1841-9364

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