Applying green analytical chemistry for rapid analysis of drugs: Adding health to pharmaceutical industry

• Main Authors: Nazrul Haq, Muzaffar Iqbal, Fars K. Alanazi, Ibrahim A. Alsarra, Faiyaz Shakeel
• Format: Article
• Language: English
• Published: Elsevier 2017-02-01
• Series: Arabian Journal of Chemistry
• Subjects: RP-HPLC; Olmesartan medoxomil; UV detection; SMEDDS; Validation
• Online Access: http://www.sciencedirect.com/science/article/pii/S187853521200295X

Green RP-HPLC method for a rapid analysis of olmesartan medoxomil (OLM) in bulk drugs, self-microemulsifying drug delivery system (SMEDDS) and marketed tablets was developed and validated in the present investigation. The chromatographic identification was achieved on Lichrosphere 250 × 4.0 mm RP C8 column having a 5 μm packing as a stationary phase using a combination of green solvents ethyl acetate:ethanol (50:50% v/v) as a mobile phase, at a flow rate of 1.0 mL/min with UV detection at 250 nm. The proposed method was validated for linearity, selectivity, accuracy, precision, reproducibility, robustness, sensitivity and specificity. The utility of the proposed method was verified by an assay of OLM in SMEDDS and commercial tablets. The proposed method was found to be selective, precise, reproducible, accurate, robust, sensitive and specific. The amount of OLM in SMEDDS and commercial tablets was found to be 101.25% and 98.67% respectively. The proposed method successfully resolved OLM peak in the presence of its degradation products which indicated stability-indicating property of the proposed method. These results indicated that the proposed method can be successfully employed for a routine analysis of OLM in bulk drugs and commercial formulations.