Identification and expression of the trehalose-6-phosphate synthase gene family members in tomato exposed to different light spectra
Light is the source of energy for plants. Light wavelengths, densities and irradiation periods act as signals directing morphological and physiological characteristics during plant growth and development. To evaluate the effects of light wavelengths on tomato growth and development, Solanum...
Main Authors: | , |
---|---|
Format: | Article |
Language: | English |
Published: |
University of Belgrade, University of Novi Sad
2017-01-01
|
Series: | Archives of Biological Sciences |
Subjects: | |
Online Access: | http://www.doiserbia.nb.rs/img/doi/0354-4664/2017/0354-46641600082C.pdf |
id |
doaj-936d75cafd11473cbd2155a2f1a1bc2e |
---|---|
record_format |
Article |
spelling |
doaj-936d75cafd11473cbd2155a2f1a1bc2e2020-11-25T01:39:09ZengUniversity of Belgrade, University of Novi SadArchives of Biological Sciences0354-46641821-43392017-01-016919310110.2298/ABS160325082C0354-46641600082CIdentification and expression of the trehalose-6-phosphate synthase gene family members in tomato exposed to different light spectraChen Zexiong0Lou Juan1Chongqing University of Arts and Sciences, College of Forestry and Life Science, Chongqing, ChinaChongqing University of Arts and Sciences, College of Forestry and Life Science, Chongqing, ChinaLight is the source of energy for plants. Light wavelengths, densities and irradiation periods act as signals directing morphological and physiological characteristics during plant growth and development. To evaluate the effects of light wavelengths on tomato growth and development, Solanum lycopersicum (cv. micro-Tom) seedlings were exposed to different light-quality environments, including white light and red light supplemented with blue light (at ratios of 3:1 and 8;1, respectively). Tomatoes grown under red light supplemented with blue light displayed significantly shorter stem length, a higher number of flower buds and rate of fruit set, but an extremely late flowering compared to white-light-grown plants. To illustrate the mechanism underlying the inhibition of stem growth and floral transition mediated by red/blue light, 10 trehalose-6-phosphate synthase (TPS) genes were identified in tomato, and bioinformatics analysis was performed. qRT-PCR analysis showed that SlTPSs were expressed widely throughout plant development and SlTPS1 was expressed at extremely high levels in stems and buds. Further analysis of several flowering-associated genes and microRNAs showed that the expressions of SlTPS1, SlFT and miR172 were significantly downregulated in tomato grown under red and blue light compared with those grown under white light, whereas miR156 transcript levels were increased. A regulatory model underlying vegetative growth and floral transition regulated by light qualities is presented. Our data provide evidence that light quality strongly affects plant growth and phase transition, most likely via the TPS1-T6P signaling pathway.http://www.doiserbia.nb.rs/img/doi/0354-4664/2017/0354-46641600082C.pdfTPSlight wavelength treatmentsvegetative growthfloral transitiontomato |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Chen Zexiong Lou Juan |
spellingShingle |
Chen Zexiong Lou Juan Identification and expression of the trehalose-6-phosphate synthase gene family members in tomato exposed to different light spectra Archives of Biological Sciences TPS light wavelength treatments vegetative growth floral transition tomato |
author_facet |
Chen Zexiong Lou Juan |
author_sort |
Chen Zexiong |
title |
Identification and expression of the trehalose-6-phosphate synthase gene family members in tomato exposed to different light spectra |
title_short |
Identification and expression of the trehalose-6-phosphate synthase gene family members in tomato exposed to different light spectra |
title_full |
Identification and expression of the trehalose-6-phosphate synthase gene family members in tomato exposed to different light spectra |
title_fullStr |
Identification and expression of the trehalose-6-phosphate synthase gene family members in tomato exposed to different light spectra |
title_full_unstemmed |
Identification and expression of the trehalose-6-phosphate synthase gene family members in tomato exposed to different light spectra |
title_sort |
identification and expression of the trehalose-6-phosphate synthase gene family members in tomato exposed to different light spectra |
publisher |
University of Belgrade, University of Novi Sad |
series |
Archives of Biological Sciences |
issn |
0354-4664 1821-4339 |
publishDate |
2017-01-01 |
description |
Light is the source of energy for plants. Light wavelengths, densities and
irradiation periods act as signals directing morphological and physiological
characteristics during plant growth and development. To evaluate the effects
of light wavelengths on tomato growth and development, Solanum lycopersicum
(cv. micro-Tom) seedlings were exposed to different light-quality
environments, including white light and red light supplemented with blue
light (at ratios of 3:1 and 8;1, respectively). Tomatoes grown under red
light supplemented with blue light displayed significantly shorter stem
length, a higher number of flower buds and rate of fruit set, but an
extremely late flowering compared to white-light-grown plants. To illustrate
the mechanism underlying the inhibition of stem growth and floral transition
mediated by red/blue light, 10 trehalose-6-phosphate synthase (TPS) genes
were identified in tomato, and bioinformatics analysis was performed. qRT-PCR
analysis showed that SlTPSs were expressed widely throughout plant
development and SlTPS1 was expressed at extremely high levels in stems and
buds. Further analysis of several flowering-associated genes and microRNAs
showed that the expressions of SlTPS1, SlFT and miR172 were significantly
downregulated in tomato grown under red and blue light compared with those
grown under white light, whereas miR156 transcript levels were increased. A
regulatory model underlying vegetative growth and floral transition regulated
by light qualities is presented. Our data provide evidence that light quality
strongly affects plant growth and phase transition, most likely via the
TPS1-T6P signaling pathway. |
topic |
TPS light wavelength treatments vegetative growth floral transition tomato |
url |
http://www.doiserbia.nb.rs/img/doi/0354-4664/2017/0354-46641600082C.pdf |
work_keys_str_mv |
AT chenzexiong identificationandexpressionofthetrehalose6phosphatesynthasegenefamilymembersintomatoexposedtodifferentlightspectra AT loujuan identificationandexpressionofthetrehalose6phosphatesynthasegenefamilymembersintomatoexposedtodifferentlightspectra |
_version_ |
1725050246062407680 |