Identification and expression of the trehalose-6-phosphate synthase gene family members in tomato exposed to different light spectra

Light is the source of energy for plants. Light wavelengths, densities and irradiation periods act as signals directing morphological and physiological characteristics during plant growth and development. To evaluate the effects of light wavelengths on tomato growth and development, Solanum...

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Main Authors: Chen Zexiong, Lou Juan
Format: Article
Language:English
Published: University of Belgrade, University of Novi Sad 2017-01-01
Series:Archives of Biological Sciences
Subjects:
TPS
Online Access:http://www.doiserbia.nb.rs/img/doi/0354-4664/2017/0354-46641600082C.pdf
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spelling doaj-936d75cafd11473cbd2155a2f1a1bc2e2020-11-25T01:39:09ZengUniversity of Belgrade, University of Novi SadArchives of Biological Sciences0354-46641821-43392017-01-016919310110.2298/ABS160325082C0354-46641600082CIdentification and expression of the trehalose-6-phosphate synthase gene family members in tomato exposed to different light spectraChen Zexiong0Lou Juan1Chongqing University of Arts and Sciences, College of Forestry and Life Science, Chongqing, ChinaChongqing University of Arts and Sciences, College of Forestry and Life Science, Chongqing, ChinaLight is the source of energy for plants. Light wavelengths, densities and irradiation periods act as signals directing morphological and physiological characteristics during plant growth and development. To evaluate the effects of light wavelengths on tomato growth and development, Solanum lycopersicum (cv. micro-Tom) seedlings were exposed to different light-quality environments, including white light and red light supplemented with blue light (at ratios of 3:1 and 8;1, respectively). Tomatoes grown under red light supplemented with blue light displayed significantly shorter stem length, a higher number of flower buds and rate of fruit set, but an extremely late flowering compared to white-light-grown plants. To illustrate the mechanism underlying the inhibition of stem growth and floral transition mediated by red/blue light, 10 trehalose-6-phosphate synthase (TPS) genes were identified in tomato, and bioinformatics analysis was performed. qRT-PCR analysis showed that SlTPSs were expressed widely throughout plant development and SlTPS1 was expressed at extremely high levels in stems and buds. Further analysis of several flowering-associated genes and microRNAs showed that the expressions of SlTPS1, SlFT and miR172 were significantly downregulated in tomato grown under red and blue light compared with those grown under white light, whereas miR156 transcript levels were increased. A regulatory model underlying vegetative growth and floral transition regulated by light qualities is presented. Our data provide evidence that light quality strongly affects plant growth and phase transition, most likely via the TPS1-T6P signaling pathway.http://www.doiserbia.nb.rs/img/doi/0354-4664/2017/0354-46641600082C.pdfTPSlight wavelength treatmentsvegetative growthfloral transitiontomato
collection DOAJ
language English
format Article
sources DOAJ
author Chen Zexiong
Lou Juan
spellingShingle Chen Zexiong
Lou Juan
Identification and expression of the trehalose-6-phosphate synthase gene family members in tomato exposed to different light spectra
Archives of Biological Sciences
TPS
light wavelength treatments
vegetative growth
floral transition
tomato
author_facet Chen Zexiong
Lou Juan
author_sort Chen Zexiong
title Identification and expression of the trehalose-6-phosphate synthase gene family members in tomato exposed to different light spectra
title_short Identification and expression of the trehalose-6-phosphate synthase gene family members in tomato exposed to different light spectra
title_full Identification and expression of the trehalose-6-phosphate synthase gene family members in tomato exposed to different light spectra
title_fullStr Identification and expression of the trehalose-6-phosphate synthase gene family members in tomato exposed to different light spectra
title_full_unstemmed Identification and expression of the trehalose-6-phosphate synthase gene family members in tomato exposed to different light spectra
title_sort identification and expression of the trehalose-6-phosphate synthase gene family members in tomato exposed to different light spectra
publisher University of Belgrade, University of Novi Sad
series Archives of Biological Sciences
issn 0354-4664
1821-4339
publishDate 2017-01-01
description Light is the source of energy for plants. Light wavelengths, densities and irradiation periods act as signals directing morphological and physiological characteristics during plant growth and development. To evaluate the effects of light wavelengths on tomato growth and development, Solanum lycopersicum (cv. micro-Tom) seedlings were exposed to different light-quality environments, including white light and red light supplemented with blue light (at ratios of 3:1 and 8;1, respectively). Tomatoes grown under red light supplemented with blue light displayed significantly shorter stem length, a higher number of flower buds and rate of fruit set, but an extremely late flowering compared to white-light-grown plants. To illustrate the mechanism underlying the inhibition of stem growth and floral transition mediated by red/blue light, 10 trehalose-6-phosphate synthase (TPS) genes were identified in tomato, and bioinformatics analysis was performed. qRT-PCR analysis showed that SlTPSs were expressed widely throughout plant development and SlTPS1 was expressed at extremely high levels in stems and buds. Further analysis of several flowering-associated genes and microRNAs showed that the expressions of SlTPS1, SlFT and miR172 were significantly downregulated in tomato grown under red and blue light compared with those grown under white light, whereas miR156 transcript levels were increased. A regulatory model underlying vegetative growth and floral transition regulated by light qualities is presented. Our data provide evidence that light quality strongly affects plant growth and phase transition, most likely via the TPS1-T6P signaling pathway.
topic TPS
light wavelength treatments
vegetative growth
floral transition
tomato
url http://www.doiserbia.nb.rs/img/doi/0354-4664/2017/0354-46641600082C.pdf
work_keys_str_mv AT chenzexiong identificationandexpressionofthetrehalose6phosphatesynthasegenefamilymembersintomatoexposedtodifferentlightspectra
AT loujuan identificationandexpressionofthetrehalose6phosphatesynthasegenefamilymembersintomatoexposedtodifferentlightspectra
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