Preliminary study on the possible impact of Thalassodendron ciliatum (Forss.) den Hartog acidic polysaccharide fractions against TAA induced liver failure

• Main Authors: Walaa S.A. Mettwally, Tamer I.M. Ragab, Abdel-Hamid A. Hamdy, Wafaa A. Helmy, Sohair Aly Hassan
• Format: Article
• Language: English
• Published: Elsevier 2021-06-01
• Series: Biomedicine & Pharmacotherapy
• Subjects: Thalassodendron ciliatum; Seagrass; Acidic polysaccharides; Antioxidant hepatoprotective; Immunomodulatory
• Online Access: http://www.sciencedirect.com/science/article/pii/S0753332221002870

The water-soluble acidic polysaccharide from Thalassodendron ciliatum (Forss.) den Hartog was successfully extracted, fractionated and purified. The phytochemical profile of the two water-soluble fractions (F1 and F2), were detected using different analytic techniques. GC-MS analysis revealed the presence of 22 saccharide. Acidic polysaccharide, galacturonic and glucuronic acid were the most abundant. Moreover, paper chromatography and electrophoresis also performed as a preliminary chemical characterization of the polymer. The hepatoprotective activity of the fractions against thioacetamide (TAA) induced liver failure; antioxidant potential and preliminary immunomodulatory activity were assigned in-vivo. The results revealed a potent competence to improve the liver function profile (ALT, AST, total bilirubin, total glyceride, etc.) and a remarkable improvement in liver architecture in comparison to the challenged intoxicated groups. Moreover, they showed high anti-oxidative properties and a promising immunomodulatory influence via Il6. These findings provide new insight into the possible role of polysaccharide purified two fractions in the treatment of acute liver injury.