Contracting CAG/CTG repeats using the CRISPR-Cas9 nickase

Contracting CAG/CTG repeats using the CRISPR-Cas9 nickase

The expansion of trinucleotide repeats has been linked to several neurodegenerative disorders. Here, the authors show that the CRISPR-Cas9 nuclease induces both expansions and contractions of the repeat region, whereas the nickase leads predominantly to contractions.

Bibliographic Details
Main Authors: Cinzia Cinesi, Lorène Aeschbach, Bin Yang, Vincent Dion
Format: Article
Language:English
Published: Nature Publishing Group 2016-11-01
Series:Nature Communications
Online Access:https://doi.org/10.1038/ncomms13272
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spelling doaj-9355183cceed4deda6f6dfaa00caf26e2021-05-11T11:06:38ZengNature Publishing GroupNature Communications2041-17232016-11-017111010.1038/ncomms13272Contracting CAG/CTG repeats using the CRISPR-Cas9 nickaseCinzia Cinesi0Lorène Aeschbach1Bin Yang2Vincent Dion3Center for Integrative Genomics, University of LausanneCenter for Integrative Genomics, University of LausanneCenter for Integrative Genomics, University of LausanneCenter for Integrative Genomics, University of LausanneThe expansion of trinucleotide repeats has been linked to several neurodegenerative disorders. Here, the authors show that the CRISPR-Cas9 nuclease induces both expansions and contractions of the repeat region, whereas the nickase leads predominantly to contractions.https://doi.org/10.1038/ncomms13272
collection DOAJ
language English
format Article
sources DOAJ
author Cinzia Cinesi
Lorène Aeschbach
Bin Yang
Vincent Dion
spellingShingle Cinzia Cinesi
Lorène Aeschbach
Bin Yang
Vincent Dion
Contracting CAG/CTG repeats using the CRISPR-Cas9 nickase
Nature Communications
author_facet Cinzia Cinesi
Lorène Aeschbach
Bin Yang
Vincent Dion
author_sort Cinzia Cinesi
title Contracting CAG/CTG repeats using the CRISPR-Cas9 nickase
title_short Contracting CAG/CTG repeats using the CRISPR-Cas9 nickase
title_full Contracting CAG/CTG repeats using the CRISPR-Cas9 nickase
title_fullStr Contracting CAG/CTG repeats using the CRISPR-Cas9 nickase
title_full_unstemmed Contracting CAG/CTG repeats using the CRISPR-Cas9 nickase
title_sort contracting cag/ctg repeats using the crispr-cas9 nickase
publisher Nature Publishing Group
series Nature Communications
issn 2041-1723
publishDate 2016-11-01
description The expansion of trinucleotide repeats has been linked to several neurodegenerative disorders. Here, the authors show that the CRISPR-Cas9 nuclease induces both expansions and contractions of the repeat region, whereas the nickase leads predominantly to contractions.
url https://doi.org/10.1038/ncomms13272
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AT loreneaeschbach contractingcagctgrepeatsusingthecrisprcas9nickase
AT binyang contractingcagctgrepeatsusingthecrisprcas9nickase
AT vincentdion contractingcagctgrepeatsusingthecrisprcas9nickase
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