Sterol and fatty acid regulatory pathways in a Giardia lamblia-derived promoter
The sterol regulatory element binding-proteins (SREBPs) are transcription factors that regulate the genes of lipid metabolism. Cholesterol and unsaturated fatty acids regulate SREBPs. Giardia lamblia (GL) is an intestinal parasite and one of the earliest derived members within the eukaryotic lineage...
| Main Authors: | , , , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Elsevier
2004-05-01
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| Series: | Journal of Lipid Research |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S002222752031840X |
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doaj-931bacc434324af1bdfc383d99af1e56 |
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Article |
| collection |
DOAJ |
| language |
English |
| format |
Article |
| sources |
DOAJ |
| author |
Tilla S. Worgall Sara R. Davis-Hayman Marissa M. Magana Peter M. Oelkers Fernando Zapata Rebecca A. Juliano Timothy F. Osborne Theodore E. Nash Richard J. Deckelbaum |
| spellingShingle |
Tilla S. Worgall Sara R. Davis-Hayman Marissa M. Magana Peter M. Oelkers Fernando Zapata Rebecca A. Juliano Timothy F. Osborne Theodore E. Nash Richard J. Deckelbaum Sterol and fatty acid regulatory pathways in a Giardia lamblia-derived promoter Journal of Lipid Research cyst-wall protein encystations sterol regulatory element binding protein |
| author_facet |
Tilla S. Worgall Sara R. Davis-Hayman Marissa M. Magana Peter M. Oelkers Fernando Zapata Rebecca A. Juliano Timothy F. Osborne Theodore E. Nash Richard J. Deckelbaum |
| author_sort |
Tilla S. Worgall |
| title |
Sterol and fatty acid regulatory pathways in a Giardia lamblia-derived promoter |
| title_short |
Sterol and fatty acid regulatory pathways in a Giardia lamblia-derived promoter |
| title_full |
Sterol and fatty acid regulatory pathways in a Giardia lamblia-derived promoter |
| title_fullStr |
Sterol and fatty acid regulatory pathways in a Giardia lamblia-derived promoter |
| title_full_unstemmed |
Sterol and fatty acid regulatory pathways in a Giardia lamblia-derived promoter |
| title_sort |
sterol and fatty acid regulatory pathways in a giardia lamblia-derived promoter |
| publisher |
Elsevier |
| series |
Journal of Lipid Research |
| issn |
0022-2275 |
| publishDate |
2004-05-01 |
| description |
The sterol regulatory element binding-proteins (SREBPs) are transcription factors that regulate the genes of lipid metabolism. Cholesterol and unsaturated fatty acids regulate SREBPs. Giardia lamblia (GL) is an intestinal parasite and one of the earliest derived members within the eukaryotic lineage. GLs exist as trophozoites and cysts. Growth in cholesterol depletion induces transcription of cyst-wall protein (CWP) genes that are upregulated during encystation. The hypothesis was investigated that SREBP-like pathways have a role in cwp gene transcription. Chinese hamster ovary cells were transfected with a cwp-2 promoter reporter construct. Incubation with cholesterol or oleate reduced cwp-2 mediated gene transcription to about half of the control. Incubation in sterol-depleted media, or in the presence of either an inhibitor of intracellular cholesterol movement or inhibitor of cholesterol synthesis, increased gene expression up to 3-fold. Overexpression of SREBPs increased reporter gene activity 2.5-fold. In the absence of functional SREBPs, cwp-2 was not regulated by cholesterol. Footprint analysis of cwp-2 reveals three novel binding sites for mammalian SREBPs with no homologies in other species or humans.The data show that SREBP binds to and can modulate transcription of a regulatory element from an ancient eukaryote and suggest the existence of an SREBP homolog in GL. |
| topic |
cyst-wall protein encystations sterol regulatory element binding protein |
| url |
http://www.sciencedirect.com/science/article/pii/S002222752031840X |
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AT tillasworgall sterolandfattyacidregulatorypathwaysinagiardialambliaderivedpromoter AT sarardavishayman sterolandfattyacidregulatorypathwaysinagiardialambliaderivedpromoter AT marissammagana sterolandfattyacidregulatorypathwaysinagiardialambliaderivedpromoter AT petermoelkers sterolandfattyacidregulatorypathwaysinagiardialambliaderivedpromoter AT fernandozapata sterolandfattyacidregulatorypathwaysinagiardialambliaderivedpromoter AT rebeccaajuliano sterolandfattyacidregulatorypathwaysinagiardialambliaderivedpromoter AT timothyfosborne sterolandfattyacidregulatorypathwaysinagiardialambliaderivedpromoter AT theodoreenash sterolandfattyacidregulatorypathwaysinagiardialambliaderivedpromoter AT richardjdeckelbaum sterolandfattyacidregulatorypathwaysinagiardialambliaderivedpromoter |
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1721506813217603584 |
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doaj-931bacc434324af1bdfc383d99af1e562021-04-27T04:40:56ZengElsevierJournal of Lipid Research0022-22752004-05-01455981988Sterol and fatty acid regulatory pathways in a Giardia lamblia-derived promoterTilla S. Worgall0Sara R. Davis-Hayman1Marissa M. Magana2Peter M. Oelkers3Fernando Zapata4Rebecca A. Juliano5Timothy F. Osborne6Theodore E. Nash7Richard J. Deckelbaum8Department of Pathology, Columbia University, New York, NY 10032; Department of Pediatrics, Columbia University, New York, NY 10032; Institute of Human Nutrition, Columbia University, New York, NY 10032; Laboratory of Parasitic Diseases, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, MD 20892; Department of Molecular Biology and Biochemistry, University of California at Irvine, Irvine, CA 92697; Department of Bioscience and Biotechnology, Drexel University, Philadelphia, PA 19104Department of Pathology, Columbia University, New York, NY 10032; Department of Pediatrics, Columbia University, New York, NY 10032; Institute of Human Nutrition, Columbia University, New York, NY 10032; Laboratory of Parasitic Diseases, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, MD 20892; Department of Molecular Biology and Biochemistry, University of California at Irvine, Irvine, CA 92697; Department of Bioscience and Biotechnology, Drexel University, Philadelphia, PA 19104Department of Pathology, Columbia University, New York, NY 10032; Department of Pediatrics, Columbia University, New York, NY 10032; Institute of Human Nutrition, Columbia University, New York, NY 10032; Laboratory of Parasitic Diseases, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, MD 20892; Department of Molecular Biology and Biochemistry, University of California at Irvine, Irvine, CA 92697; Department of Bioscience and Biotechnology, Drexel University, Philadelphia, PA 19104Department of Pathology, Columbia University, New York, NY 10032; Department of Pediatrics, Columbia University, New York, NY 10032; Institute of Human Nutrition, Columbia University, New York, NY 10032; Laboratory of Parasitic Diseases, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, MD 20892; Department of Molecular Biology and Biochemistry, University of California at Irvine, Irvine, CA 92697; Department of Bioscience and Biotechnology, Drexel University, Philadelphia, PA 19104Department of Pathology, Columbia University, New York, NY 10032; Department of Pediatrics, Columbia University, New York, NY 10032; Institute of Human Nutrition, Columbia University, New York, NY 10032; Laboratory of Parasitic Diseases, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, MD 20892; Department of Molecular Biology and Biochemistry, University of California at Irvine, Irvine, CA 92697; Department of Bioscience and Biotechnology, Drexel University, Philadelphia, PA 19104Department of Pathology, Columbia University, New York, NY 10032; Department of Pediatrics, Columbia University, New York, NY 10032; Institute of Human Nutrition, Columbia University, New York, NY 10032; Laboratory of Parasitic Diseases, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, MD 20892; Department of Molecular Biology and Biochemistry, University of California at Irvine, Irvine, CA 92697; Department of Bioscience and Biotechnology, Drexel University, Philadelphia, PA 19104Department of Pathology, Columbia University, New York, NY 10032; Department of Pediatrics, Columbia University, New York, NY 10032; Institute of Human Nutrition, Columbia University, New York, NY 10032; Laboratory of Parasitic Diseases, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, MD 20892; Department of Molecular Biology and Biochemistry, University of California at Irvine, Irvine, CA 92697; Department of Bioscience and Biotechnology, Drexel University, Philadelphia, PA 19104Department of Pathology, Columbia University, New York, NY 10032; Department of Pediatrics, Columbia University, New York, NY 10032; Institute of Human Nutrition, Columbia University, New York, NY 10032; Laboratory of Parasitic Diseases, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, MD 20892; Department of Molecular Biology and Biochemistry, University of California at Irvine, Irvine, CA 92697; Department of Bioscience and Biotechnology, Drexel University, Philadelphia, PA 19104Department of Pathology, Columbia University, New York, NY 10032; Department of Pediatrics, Columbia University, New York, NY 10032; Institute of Human Nutrition, Columbia University, New York, NY 10032; Laboratory of Parasitic Diseases, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, MD 20892; Department of Molecular Biology and Biochemistry, University of California at Irvine, Irvine, CA 92697; Department of Bioscience and Biotechnology, Drexel University, Philadelphia, PA 19104The sterol regulatory element binding-proteins (SREBPs) are transcription factors that regulate the genes of lipid metabolism. Cholesterol and unsaturated fatty acids regulate SREBPs. Giardia lamblia (GL) is an intestinal parasite and one of the earliest derived members within the eukaryotic lineage. GLs exist as trophozoites and cysts. Growth in cholesterol depletion induces transcription of cyst-wall protein (CWP) genes that are upregulated during encystation. The hypothesis was investigated that SREBP-like pathways have a role in cwp gene transcription. Chinese hamster ovary cells were transfected with a cwp-2 promoter reporter construct. Incubation with cholesterol or oleate reduced cwp-2 mediated gene transcription to about half of the control. Incubation in sterol-depleted media, or in the presence of either an inhibitor of intracellular cholesterol movement or inhibitor of cholesterol synthesis, increased gene expression up to 3-fold. Overexpression of SREBPs increased reporter gene activity 2.5-fold. In the absence of functional SREBPs, cwp-2 was not regulated by cholesterol. Footprint analysis of cwp-2 reveals three novel binding sites for mammalian SREBPs with no homologies in other species or humans.The data show that SREBP binds to and can modulate transcription of a regulatory element from an ancient eukaryote and suggest the existence of an SREBP homolog in GL.http://www.sciencedirect.com/science/article/pii/S002222752031840Xcyst-wall proteinencystationssterol regulatory element binding protein |