Development of Si-based electrical biosensors: Simulations and first experimental results

• Main Authors: Marco Favetta, Antonio Valletta, Guglielmo Fortunato, Maria Eloisa Castagna, Sabrina Conoci, Emanuele Luigi Sciuto, Tiziana Cosentino, Fulvia Sinatra, Sebania Libertino
• Format: Article
• Language: English
• Published: Elsevier 2015-12-01
• Series: Sensing and Bio-Sensing Research
• Online Access: http://www.sciencedirect.com/science/article/pii/S2214180415300374

In this work, we simulated and experimentally assessed the possibility to detect, through electrical transduction, hybridization of DNA molecules on MOS-like devices, having different dielectrics: SiO2, Si3N4 and SiO2/Si3N4/SiO2 (ONO). The electrical characterization was performed after the various functionalization steps, consisting of dielectric activation, silanization, DNA spotting and anchoring, and after the hybridization process, to test the devices effectiveness as DNA recognition biosensors. The experimental results were used to validate device simulations. The comparison shows the ability to determine a priori the DNA probe density needed to maximize the response. The results confirm that the structures analyzed are sensitive to the immobilization of DNA and its hybridization.