Generation of versatile ss-dsDNA hybrid substrates for single-molecule analysis

Generation of versatile ss-dsDNA hybrid substrates for single-molecule analysis

Summary: Here, we describe a rapid and versatile protocol to generate gapped DNA substrates for single-molecule (SM) analysis using optical tweezers via site-specific Cas9 nicking and force-induced melting. We provide examples of single-stranded (ss) DNA gaps of different length and position. We out...

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Bibliographic Details
Main Authors: Ondrej Belan, George Moore, Artur Kaczmarczyk, Matthew D. Newton, Roopesh Anand, Simon J. Boulton, David S. Rueda
Format: Article
Language:English
Published: Elsevier 2021-06-01
Series:STAR Protocols
Subjects:
Single-molecule Assays
Microscopy
Molecular/Chemical Probes
Online Access:http://www.sciencedirect.com/science/article/pii/S2666166721002951

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