Ostreopsis cf. ovata (Dinophyceae) Molecular Phylogeny, Morphology, and Detection of Ovatoxins in Strains and Field Samples from Brazil

Recurrent blooms of Ostreopsis cf. ovata have been reported in Brazil and the Mediterranean Sea with associated ecological, and in the latter case, health impacts. Molecular data based on the D1−D3 and D8−D10 regions of the LSU rDNA and ITS l...

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Main Authors:	Silvia M. Nascimento, Raquel A. F. Neves, Gabriela A. L. De’Carli, Geovanna T. Borsato, Rodrigo A. F. da Silva, Guilherme A. Melo, Agatha M. de Morais, Thais C. Cockell, Santiago Fraga, Adriana D. Menezes-Salgueiro, Luiz L. Mafra, Philipp Hess, Fabiano Salgueiro
Format:	Article
Language:	English
Published:	MDPI AG 2020-01-01
Series:	Toxins
Subjects:	benthic dinoflagellates harmful algal blooms ovatoxins taxonomy morphometry
Online Access:	https://www.mdpi.com/2072-6651/12/2/70

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spelling	doaj-92e676bcd06d4247946182c11d7f07892020-11-25T02:05:44ZengMDPI AGToxins2072-66512020-01-011227010.3390/toxins12020070toxins12020070<i>Ostreopsis</i> cf. <i>ovata</i> (Dinophyceae) Molecular Phylogeny, Morphology, and Detection of Ovatoxins in Strains and Field Samples from BrazilSilvia M. Nascimento0Raquel A. F. Neves1Gabriela A. L. De’Carli2Geovanna T. Borsato3Rodrigo A. F. da Silva4Guilherme A. Melo5Agatha M. de Morais6Thais C. Cockell7Santiago Fraga8Adriana D. Menezes-Salgueiro9Luiz L. Mafra10Philipp Hess11Fabiano Salgueiro12Laboratório de Microalgas Marinhas, Universidade Federal do Estado do Rio de Janeiro (UNIRIO), Av. Pasteur, 458, 314-B, Rio de Janeiro, RJ 22290-240, BrazilLaboratório de Microalgas Marinhas, Universidade Federal do Estado do Rio de Janeiro (UNIRIO), Av. Pasteur, 458, 314-B, Rio de Janeiro, RJ 22290-240, BrazilLaboratório de Microalgas Marinhas, Universidade Federal do Estado do Rio de Janeiro (UNIRIO), Av. Pasteur, 458, 314-B, Rio de Janeiro, RJ 22290-240, BrazilLaboratório de Microalgas Marinhas, Universidade Federal do Estado do Rio de Janeiro (UNIRIO), Av. Pasteur, 458, 314-B, Rio de Janeiro, RJ 22290-240, BrazilLaboratório de Microalgas Marinhas, Universidade Federal do Estado do Rio de Janeiro (UNIRIO), Av. Pasteur, 458, 314-B, Rio de Janeiro, RJ 22290-240, BrazilLaboratório de Biodiversidade e Evolução Molecular, Universidade Federal do Estado do Rio de Janeiro (UNIRIO), Av. Pasteur, 458, 512, Rio de Janeiro, RJ 22290-240, BrazilLaboratório de Microalgas Marinhas, Universidade Federal do Estado do Rio de Janeiro (UNIRIO), Av. Pasteur, 458, 314-B, Rio de Janeiro, RJ 22290-240, BrazilLaboratório de Microalgas Marinhas, Universidade Federal do Estado do Rio de Janeiro (UNIRIO), Av. Pasteur, 458, 314-B, Rio de Janeiro, RJ 22290-240, BrazilCentro Oceanográfico de Vigo, Instituto Español de Oceanografía (IEO), Subida a Radio Faro 50, 36390 Vigo, SpainLaboratório de Biotecnologia Vegetal, Instituto Federal de Educação, Ciência e Tecnologia do Rio de Janeiro, Rua Senador Furtado, 121, 112. Maracanã, Rio de Janeiro, RJ 20270-021, BrazilCentro de Estudos do Mar, Universidade Federal do Paraná, Cx. Postal 61, Pontal do Paraná, PR 83255-976, BrazilIFREMER, Laboratoire Phycotoxines, Rue de l’Ile d’Yeu, 44311 Nantes, FranceLaboratório de Biodiversidade e Evolução Molecular, Universidade Federal do Estado do Rio de Janeiro (UNIRIO), Av. Pasteur, 458, 512, Rio de Janeiro, RJ 22290-240, BrazilRecurrent blooms of <i>Ostreopsis</i> cf. <i>ovata</i> have been reported in Brazil and the Mediterranean Sea with associated ecological, and in the latter case, health impacts. Molecular data based on the D1−D3 and D8−D10 regions of the LSU rDNA and ITS loci, and the morphology of <i>O</i>. cf. <i>ovata</i> isolates and field populations from locations along the Brazilian tropical and subtropical coastal regions and three oceanic islands are presented. Additional ITS sequences from three single cells from the tropical coast are provided. Toxin profiles and quantities of PLTX and their analogues; OVTXs; contained in cells from two clonal cultures and two field blooms from Rio de Janeiro were investigated. Morphology was examined using both light and epifluorescence microscopy. Morphometric analysis of different strains and field populations from diverse locations were compared. Molecular analysis showed that six of the seven sequences grouped at the large “Atlantic/Mediterranean/Pacific” sub-clade, while one sequence branched in a sister clade with sequences from Madeira Island and Greece. The toxin profile of strains and bloom field samples from Rio de Janeiro were dominated by OVTX-a and -b, with total cell quotas (31.3 and 39.3 pg cell<sup>−1</sup>) in the range of that previously reported for strains of <i>O</i>. cf. <i>ovata</i>.https://www.mdpi.com/2072-6651/12/2/70benthic dinoflagellatesharmful algal bloomsovatoxinstaxonomymorphometry
collection	DOAJ
language	English
format	Article
sources	DOAJ
author	Silvia M. Nascimento Raquel A. F. Neves Gabriela A. L. De’Carli Geovanna T. Borsato Rodrigo A. F. da Silva Guilherme A. Melo Agatha M. de Morais Thais C. Cockell Santiago Fraga Adriana D. Menezes-Salgueiro Luiz L. Mafra Philipp Hess Fabiano Salgueiro
spellingShingle	Silvia M. Nascimento Raquel A. F. Neves Gabriela A. L. De’Carli Geovanna T. Borsato Rodrigo A. F. da Silva Guilherme A. Melo Agatha M. de Morais Thais C. Cockell Santiago Fraga Adriana D. Menezes-Salgueiro Luiz L. Mafra Philipp Hess Fabiano Salgueiro <i>Ostreopsis</i> cf. <i>ovata</i> (Dinophyceae) Molecular Phylogeny, Morphology, and Detection of Ovatoxins in Strains and Field Samples from Brazil Toxins benthic dinoflagellates harmful algal blooms ovatoxins taxonomy morphometry
author_facet	Silvia M. Nascimento Raquel A. F. Neves Gabriela A. L. De’Carli Geovanna T. Borsato Rodrigo A. F. da Silva Guilherme A. Melo Agatha M. de Morais Thais C. Cockell Santiago Fraga Adriana D. Menezes-Salgueiro Luiz L. Mafra Philipp Hess Fabiano Salgueiro
author_sort	Silvia M. Nascimento
title	<i>Ostreopsis</i> cf. <i>ovata</i> (Dinophyceae) Molecular Phylogeny, Morphology, and Detection of Ovatoxins in Strains and Field Samples from Brazil
title_short	<i>Ostreopsis</i> cf. <i>ovata</i> (Dinophyceae) Molecular Phylogeny, Morphology, and Detection of Ovatoxins in Strains and Field Samples from Brazil
title_full	<i>Ostreopsis</i> cf. <i>ovata</i> (Dinophyceae) Molecular Phylogeny, Morphology, and Detection of Ovatoxins in Strains and Field Samples from Brazil
title_fullStr	<i>Ostreopsis</i> cf. <i>ovata</i> (Dinophyceae) Molecular Phylogeny, Morphology, and Detection of Ovatoxins in Strains and Field Samples from Brazil
title_full_unstemmed	<i>Ostreopsis</i> cf. <i>ovata</i> (Dinophyceae) Molecular Phylogeny, Morphology, and Detection of Ovatoxins in Strains and Field Samples from Brazil
title_sort	<i>ostreopsis</i> cf. <i>ovata</i> (dinophyceae) molecular phylogeny, morphology, and detection of ovatoxins in strains and field samples from brazil
publisher	MDPI AG
series	Toxins
issn	2072-6651
publishDate	2020-01-01
description	Recurrent blooms of <i>Ostreopsis</i> cf. <i>ovata</i> have been reported in Brazil and the Mediterranean Sea with associated ecological, and in the latter case, health impacts. Molecular data based on the D1−D3 and D8−D10 regions of the LSU rDNA and ITS loci, and the morphology of <i>O</i>. cf. <i>ovata</i> isolates and field populations from locations along the Brazilian tropical and subtropical coastal regions and three oceanic islands are presented. Additional ITS sequences from three single cells from the tropical coast are provided. Toxin profiles and quantities of PLTX and their analogues; OVTXs; contained in cells from two clonal cultures and two field blooms from Rio de Janeiro were investigated. Morphology was examined using both light and epifluorescence microscopy. Morphometric analysis of different strains and field populations from diverse locations were compared. Molecular analysis showed that six of the seven sequences grouped at the large “Atlantic/Mediterranean/Pacific” sub-clade, while one sequence branched in a sister clade with sequences from Madeira Island and Greece. The toxin profile of strains and bloom field samples from Rio de Janeiro were dominated by OVTX-a and -b, with total cell quotas (31.3 and 39.3 pg cell<sup>−1</sup>) in the range of that previously reported for strains of <i>O</i>. cf. <i>ovata</i>.
topic	benthic dinoflagellates harmful algal blooms ovatoxins taxonomy morphometry
url	https://www.mdpi.com/2072-6651/12/2/70
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<i>Ostreopsis</i> cf. <i>ovata</i> (Dinophyceae) Molecular Phylogeny, Morphology, and Detection of Ovatoxins in Strains and Field Samples from Brazil

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