Use of anchorchip-time-of-flight spectrometry technology to screen tumor biomarker proteins in serum for small cell lung cancer

• Main Authors: Huo Shufen, Bu Lina, Nan Yandong, Lin Xiuli, Yang Shuanying, Du Jie, Shang Wenli
• Format: Article
• Language: English
• Published: BMC 2010-09-01
• Series: Diagnostic Pathology
• Online Access: http://www.diagnosticpathology.org/content/5/1/60
• ISSN: 1746-1596

<p>Abstract</p> <p>Background</p> <p>The purpose of this study is to discover potential biomarkers in serum for the detection of small cell lung cancer (SCLC).</p> <p>Methods</p> <p>74 serum samples including 30 from SCLC patients and 44 from healthy controls were analyzed using ClinProt system combined with matrix-assisted laser desorption/ionization time-of-flight masss spectrometry (MALDI-TOF-MS). ClinProt software and genetic algorithm analysis selected a panel of serum markers that most efficiently predicted which patients had SCLC.</p> <p>Results</p> <p>The diagnostic pattern combined with 5 potential biomarkers could differentiate SCLC patients from healthy persons, with a sensitivity of 90%, specificity of 97.73%. Remarkably, 88.89% of stage I/II patients were accurately assigned to SCLC.</p> <p>Conclusions</p> <p>Anchorchip-time-of-flight spectrometry technology will provide a highly accurate approach for discovering new biomarkers for the detection of SCLC.</p>