An immunoassay for the measurement of (1→3)-β-D-glucans in the indoor environment

An inhibition enzyme immunoassay was developed for quantitation of (1→3)-β-D-glucans in the indoor environment. Immunospecific rabbit antibodies were produced by immunization with bovine serum albuminconjugated laminarin.The laminarin calibration curve ranged from 40 to 3000 ng/ml.Another (1→3)-β-D-...

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Main Authors:	Jeroen Douwes, Gert Doekes, Roy Montijn, Dick Heederik, Bert Brunekreef
Format:	Article
Language:	English
Published:	Hindawi Limited 1997-01-01
Series:	Mediators of Inflammation
Online Access:	http://dx.doi.org/10.1080/09629359791578

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spelling	doaj-92a7355f8eb840cba29f8d62e49eee352020-11-24T21:04:21ZengHindawi LimitedMediators of Inflammation0962-93511466-18611997-01-016425726210.1080/09629359791578An immunoassay for the measurement of (1→3)-β-D-glucans in the indoor environmentJeroen Douwes0Gert Doekes1Roy Montijn2Dick Heederik3Bert Brunekreef4Environmental and Occupational Health, Department of Environmental Sciences, Wageningen Agricultural University, PO Box 238, Wageningen 6700 AE , The NetherlandsEnvironmental and Occupational Health, Department of Environmental Sciences, Wageningen Agricultural University, PO Box 238, Wageningen 6700 AE , The NetherlandsInstitute of Molecular Cell Biology, BioCentrum Amsterdam, University of Amsterdam, The NetherlandsEnvironmental and Occupational Health, Department of Environmental Sciences, Wageningen Agricultural University, PO Box 238, Wageningen 6700 AE , The NetherlandsEnvironmental and Occupational Health, Department of Environmental Sciences, Wageningen Agricultural University, PO Box 238, Wageningen 6700 AE , The NetherlandsAn inhibition enzyme immunoassay was developed for quantitation of (1→3)-β-D-glucans in the indoor environment. Immunospecific rabbit antibodies were produced by immunization with bovine serum albuminconjugated laminarin.The laminarin calibration curve ranged from 40 to 3000 ng/ml.Another (1→3)-β-D-glucan (curdlan) showed a similar inhibition curve, but was less reactive on a weight basis. Pustulan, presumed to be (1→3)-β-D-glucan, also showed immunoreactivity in the assay. Control experiments indicated that this was due to (1→3)-β-D-glucan structures. Other non-(1→3)-β-D-glucan polysaccharides did not react. (1→3)-β-Dglucan was detectable in dust from a variety of occupational and environmental settings. We conclude that the new assay offers a useful method for indoor (1→3)-β-Dglucan exposure assessment.http://dx.doi.org/10.1080/09629359791578
collection	DOAJ
language	English
format	Article
sources	DOAJ
author	Jeroen Douwes Gert Doekes Roy Montijn Dick Heederik Bert Brunekreef
spellingShingle	Jeroen Douwes Gert Doekes Roy Montijn Dick Heederik Bert Brunekreef An immunoassay for the measurement of (1→3)-β-D-glucans in the indoor environment Mediators of Inflammation
author_facet	Jeroen Douwes Gert Doekes Roy Montijn Dick Heederik Bert Brunekreef
author_sort	Jeroen Douwes
title	An immunoassay for the measurement of (1→3)-β-D-glucans in the indoor environment
title_short	An immunoassay for the measurement of (1→3)-β-D-glucans in the indoor environment
title_full	An immunoassay for the measurement of (1→3)-β-D-glucans in the indoor environment
title_fullStr	An immunoassay for the measurement of (1→3)-β-D-glucans in the indoor environment
title_full_unstemmed	An immunoassay for the measurement of (1→3)-β-D-glucans in the indoor environment
title_sort	immunoassay for the measurement of (1→3)-β-d-glucans in the indoor environment
publisher	Hindawi Limited
series	Mediators of Inflammation
issn	0962-9351 1466-1861
publishDate	1997-01-01
description	An inhibition enzyme immunoassay was developed for quantitation of (1→3)-β-D-glucans in the indoor environment. Immunospecific rabbit antibodies were produced by immunization with bovine serum albuminconjugated laminarin.The laminarin calibration curve ranged from 40 to 3000 ng/ml.Another (1→3)-β-D-glucan (curdlan) showed a similar inhibition curve, but was less reactive on a weight basis. Pustulan, presumed to be (1→3)-β-D-glucan, also showed immunoreactivity in the assay. Control experiments indicated that this was due to (1→3)-β-D-glucan structures. Other non-(1→3)-β-D-glucan polysaccharides did not react. (1→3)-β-Dglucan was detectable in dust from a variety of occupational and environmental settings. We conclude that the new assay offers a useful method for indoor (1→3)-β-Dglucan exposure assessment.
url	http://dx.doi.org/10.1080/09629359791578
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An immunoassay for the measurement of (1→3)-β-D-glucans in the indoor environment

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