Circular polymerase extension cloning of complex gene libraries and pathways.

High-throughput genomics and the emerging field of synthetic biology demand ever more convenient, economical, and efficient technologies to assemble and clone genes, gene libraries and synthetic pathways. Here, we describe the development of a novel and extremely simple cloning method, circular poly...

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Bibliographic Details
Main Authors: Jiayuan Quan, Jingdong Tian
Format: Article
Language:English
Published: Public Library of Science (PLoS) 2009-07-01
Series:PLoS ONE
Online Access:https://www.ncbi.nlm.nih.gov/pmc/articles/pmid/19649325/pdf/?tool=EBI