IDENTIFICATION OF GRAM-NEGATIVE BACTERIA OF FISH BY THE METHOD OF PCR-RFLP OF 16S rDNA GENE

IDENTIFICATION OF GRAM-NEGATIVE BACTERIA OF FISH BY THE METHOD OF PCR-RFLP OF 16S rDNA GENE

The complex method of polymerase chain reaction and restriction fragment length polymorphism (PCR-RFLP) targeting to the 16S rDNA gene was developed for identification of the most widespread gram-negative bacterias of fish. The specificity and efficiency of flexible oligonucleotide primers for 16S r...

Full description

Bibliographic Details
Main Author: Yu. Rud
Format: Article
Language:English
Published: Institute of Fisheries 2013-03-01
Series:Ribogospodarsʹka Nauka Ukraïni
Subjects:
PCR-RFLP
16s rRNA
gram-negative (G-) bacteria
Online Access:http://fsu.ua/index.php/en/2013/1-2013-23/2013-01-080-086
id doaj-9286de8205a44abe9aa61e9ada0e1980
record_format Article
spelling doaj-9286de8205a44abe9aa61e9ada0e19802020-11-24T20:59:12ZengInstitute of Fisheries Ribogospodarsʹka Nauka Ukraïni2075-15082312-95812013-03-011(23)808610.15407/fsu2013.01.080IDENTIFICATION OF GRAM-NEGATIVE BACTERIA OF FISH BY THE METHOD OF PCR-RFLP OF 16S rDNA GENEYu. Rud0Institute of Fisheries NAAS, KyivThe complex method of polymerase chain reaction and restriction fragment length polymorphism (PCR-RFLP) targeting to the 16S rDNA gene was developed for identification of the most widespread gram-negative bacterias of fish. The specificity and efficiency of flexible oligonucleotide primers for 16S rDNA gene of gram-negative bacteria and also possibility of differentiation of these bacteria by use of the restriction endonuclease sets were shown.http://fsu.ua/index.php/en/2013/1-2013-23/2013-01-080-086PCR-RFLP16s rRNAgram-negative (G-) bacteria
collection DOAJ
language English
format Article
sources DOAJ
author Yu. Rud
spellingShingle Yu. Rud
IDENTIFICATION OF GRAM-NEGATIVE BACTERIA OF FISH BY THE METHOD OF PCR-RFLP OF 16S rDNA GENE
Ribogospodarsʹka Nauka Ukraïni
PCR-RFLP
16s rRNA
gram-negative (G-) bacteria
author_facet Yu. Rud
author_sort Yu. Rud
title IDENTIFICATION OF GRAM-NEGATIVE BACTERIA OF FISH BY THE METHOD OF PCR-RFLP OF 16S rDNA GENE
title_short IDENTIFICATION OF GRAM-NEGATIVE BACTERIA OF FISH BY THE METHOD OF PCR-RFLP OF 16S rDNA GENE
title_full IDENTIFICATION OF GRAM-NEGATIVE BACTERIA OF FISH BY THE METHOD OF PCR-RFLP OF 16S rDNA GENE
title_fullStr IDENTIFICATION OF GRAM-NEGATIVE BACTERIA OF FISH BY THE METHOD OF PCR-RFLP OF 16S rDNA GENE
title_full_unstemmed IDENTIFICATION OF GRAM-NEGATIVE BACTERIA OF FISH BY THE METHOD OF PCR-RFLP OF 16S rDNA GENE
title_sort identification of gram-negative bacteria of fish by the method of pcr-rflp of 16s rdna gene
publisher Institute of Fisheries
series Ribogospodarsʹka Nauka Ukraïni
issn 2075-1508
2312-9581
publishDate 2013-03-01
description The complex method of polymerase chain reaction and restriction fragment length polymorphism (PCR-RFLP) targeting to the 16S rDNA gene was developed for identification of the most widespread gram-negative bacterias of fish. The specificity and efficiency of flexible oligonucleotide primers for 16S rDNA gene of gram-negative bacteria and also possibility of differentiation of these bacteria by use of the restriction endonuclease sets were shown.
topic PCR-RFLP
16s rRNA
gram-negative (G-) bacteria
url http://fsu.ua/index.php/en/2013/1-2013-23/2013-01-080-086
work_keys_str_mv AT yurud identificationofgramnegativebacteriaoffishbythemethodofpcrrflpof16srdnagene
_version_ 1716783360370540544

Similar Items