Structural Analysis of Cytochrome P450 105N1 Involved in the Biosynthesis of the Zincophore, Coelibactin

• Main Authors: Bin Zhao, Suzy C. Moody, Robert C. Hider, Li Lei, Steven L. Kelly, Michael R. Waterman, David C. Lamb
• Format: Article
• Language: English
• Published: MDPI AG 2012-07-01
• Series: International Journal of Molecular Sciences
• Subjects: cytochrome P450; CYP105N1; siderophore; <em>Streptomyces coelicolor</em> A3(2); zinc chelation
• Online Access: http://www.mdpi.com/1422-0067/13/7/8500
• ISSN: 1422-0067

Coelibactin is a putative non-ribosomally synthesized peptide with predicted zincophore activity and which has been implicated in antibiotic regulation in <em>Streptomyces coelicolor</em> A3(2). The coelibactin biosynthetic pathway contains a stereo- and regio-specific monooxygenation step catalyzed by a cytochrome P450 enzyme (CYP105N1). We have determined the X-ray crystal structure of CYP105N1 at 2.9 Å and analyzed it in the context of the bacterial CYP105 family as a whole. The crystal structure reveals a channel between the α-helical domain and the β-sheet domain exposing the heme pocket and the long helix I to the solvent. This wide-open conformation of CYP105N1 may be related to the bulky substrate coelibactin. The ligand-free CYP105N1 structure has enough room in the substrate access channel to allow the coelibactin to enter into the active site. Analysis of typical siderophore ligands suggests that CYP105N1 may produce derivatives of coelibactin, which would then be able to chelate the zinc divalent cation.