Characterisation of sixteen additional polymorphic microsatellite loci for the spreading but locally rare European butterfly, Brenthis ino (Lepidoptera: Nymphalidae)

Whilst the overall geographic range of the lesser marbled fritillary, Brenthis ino (Rottemburg, 1775), is currently expanding, this species is patchily distributed at a local spatial scale due to its use of flower rich semi-natural meadows and the aggregated distribution of its host plant. Therefore...

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Main Authors: Christophe LEBIGRE, Camille TURLURE, Nicolas SCHTICKZELLE
Format: Article
Language:English
Published: Institute of Entomology, Biology Centre, Czech Academy of Science 2015-04-01
Series:European Journal of Entomology
Subjects:
Online Access:https://www.eje.cz/artkey/eje-201502-0020_Characterisation_of_sixteen_additional_polymorphic_microsatellite_loci_for_the_spreading_but_locally_rare_Europ.php
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spelling doaj-91e758736cf949219503edaff712c2492021-04-16T20:35:35ZengInstitute of Entomology, Biology Centre, Czech Academy of ScienceEuropean Journal of Entomology1210-57591802-88292015-04-01112238939210.14411/eje.2015.047eje-201502-0020Characterisation of sixteen additional polymorphic microsatellite loci for the spreading but locally rare European butterfly, Brenthis ino (Lepidoptera: Nymphalidae)Christophe LEBIGRE0Camille TURLURE1Nicolas SCHTICKZELLE2Earth and Life Institute, Biodiversity Research Centre, Université catholique de Louvain, Croix du Sud 4, L7.07.04, B-1348 Louvain-la-Neuve, Belgium; e-mails: christophe.lebigre@uclouvain.be; camille.turlure@uclouvain.be; nicolas.schtickzelle@uclouvain.beEarth and Life Institute, Biodiversity Research Centre, Université catholique de Louvain, Croix du Sud 4, L7.07.04, B-1348 Louvain-la-Neuve, Belgium; e-mails: christophe.lebigre@uclouvain.be; camille.turlure@uclouvain.be; nicolas.schtickzelle@uclouvain.beEarth and Life Institute, Biodiversity Research Centre, Université catholique de Louvain, Croix du Sud 4, L7.07.04, B-1348 Louvain-la-Neuve, Belgium; e-mails: christophe.lebigre@uclouvain.be; camille.turlure@uclouvain.be; nicolas.schtickzelle@uclouvain.beWhilst the overall geographic range of the lesser marbled fritillary, Brenthis ino (Rottemburg, 1775), is currently expanding, this species is patchily distributed at a local spatial scale due to its use of flower rich semi-natural meadows and the aggregated distribution of its host plant. Therefore, understanding the dispersal patterns of this butterfly and the effect of increasing fragmentation of the landscapes in central and Western Europe on its population dynamics is key to determining whether this patchy distribution can lead to metapopulation structuring and dynamics. One way to determine the degree to which local populations are isolated from one another is to use high resolution molecular genetic markers and thence quantify gene flow and genetic drift. Eleven microsatellite loci have previously been developed for this species, but six showed evidence of null alleles, effectively violating key assumptions of the models used to infer gene flow. We therefore developed a set of new primer pairs to amplify a suite of 16 highly polymorphic microsatellite loci (number of alleles ranged from 2-30) of which nine were found to conform to the Hardy-Weinberg's expectations, whilst at the same time not showing any clear signature for the presence of null alleles. We further describe how these primers were optimized for landscape and metapopulation genetics studies in Belgian Ardenne.https://www.eje.cz/artkey/eje-201502-0020_Characterisation_of_sixteen_additional_polymorphic_microsatellite_loci_for_the_spreading_but_locally_rare_Europ.phplepidopteranymphalidaebrenthis inolesser marbled fritillaryconnectivityinbreedinglandscape geneticsmicrosatellitenull alleles
collection DOAJ
language English
format Article
sources DOAJ
author Christophe LEBIGRE
Camille TURLURE
Nicolas SCHTICKZELLE
spellingShingle Christophe LEBIGRE
Camille TURLURE
Nicolas SCHTICKZELLE
Characterisation of sixteen additional polymorphic microsatellite loci for the spreading but locally rare European butterfly, Brenthis ino (Lepidoptera: Nymphalidae)
European Journal of Entomology
lepidoptera
nymphalidae
brenthis ino
lesser marbled fritillary
connectivity
inbreeding
landscape genetics
microsatellite
null alleles
author_facet Christophe LEBIGRE
Camille TURLURE
Nicolas SCHTICKZELLE
author_sort Christophe LEBIGRE
title Characterisation of sixteen additional polymorphic microsatellite loci for the spreading but locally rare European butterfly, Brenthis ino (Lepidoptera: Nymphalidae)
title_short Characterisation of sixteen additional polymorphic microsatellite loci for the spreading but locally rare European butterfly, Brenthis ino (Lepidoptera: Nymphalidae)
title_full Characterisation of sixteen additional polymorphic microsatellite loci for the spreading but locally rare European butterfly, Brenthis ino (Lepidoptera: Nymphalidae)
title_fullStr Characterisation of sixteen additional polymorphic microsatellite loci for the spreading but locally rare European butterfly, Brenthis ino (Lepidoptera: Nymphalidae)
title_full_unstemmed Characterisation of sixteen additional polymorphic microsatellite loci for the spreading but locally rare European butterfly, Brenthis ino (Lepidoptera: Nymphalidae)
title_sort characterisation of sixteen additional polymorphic microsatellite loci for the spreading but locally rare european butterfly, brenthis ino (lepidoptera: nymphalidae)
publisher Institute of Entomology, Biology Centre, Czech Academy of Science
series European Journal of Entomology
issn 1210-5759
1802-8829
publishDate 2015-04-01
description Whilst the overall geographic range of the lesser marbled fritillary, Brenthis ino (Rottemburg, 1775), is currently expanding, this species is patchily distributed at a local spatial scale due to its use of flower rich semi-natural meadows and the aggregated distribution of its host plant. Therefore, understanding the dispersal patterns of this butterfly and the effect of increasing fragmentation of the landscapes in central and Western Europe on its population dynamics is key to determining whether this patchy distribution can lead to metapopulation structuring and dynamics. One way to determine the degree to which local populations are isolated from one another is to use high resolution molecular genetic markers and thence quantify gene flow and genetic drift. Eleven microsatellite loci have previously been developed for this species, but six showed evidence of null alleles, effectively violating key assumptions of the models used to infer gene flow. We therefore developed a set of new primer pairs to amplify a suite of 16 highly polymorphic microsatellite loci (number of alleles ranged from 2-30) of which nine were found to conform to the Hardy-Weinberg's expectations, whilst at the same time not showing any clear signature for the presence of null alleles. We further describe how these primers were optimized for landscape and metapopulation genetics studies in Belgian Ardenne.
topic lepidoptera
nymphalidae
brenthis ino
lesser marbled fritillary
connectivity
inbreeding
landscape genetics
microsatellite
null alleles
url https://www.eje.cz/artkey/eje-201502-0020_Characterisation_of_sixteen_additional_polymorphic_microsatellite_loci_for_the_spreading_but_locally_rare_Europ.php
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