The Effects of Addition of Mononucleotides on Sma nuc Endonuclease Activity

The Effects of Addition of Mononucleotides on Sma nuc Endonuclease Activity

Examination of the effects of mononucleotides on Sma nuc endonuclease originated from Gram negative bacterium Serratia marcescens displayed that any mononucleotide produced by Sma nuc during hydrolysis of DNA or RNA may regulate the enzyme activity affecting the RNase activity without pronounced inf...

Full description

Bibliographic Details
Main Authors: Julia Romanova, Maria Filimonova
Format: Article
Language:English
Published: Hindawi Limited 2012-01-01
Series:The Scientific World Journal
Online Access:http://dx.doi.org/10.1100/2012/454176
id doaj-91e25511d74e4769b4a5669d265ab87b
record_format Article
spelling doaj-91e25511d74e4769b4a5669d265ab87b2020-11-25T01:33:27ZengHindawi LimitedThe Scientific World Journal1537-744X2012-01-01201210.1100/2012/454176454176The Effects of Addition of Mononucleotides on Sma nuc Endonuclease ActivityJulia Romanova0Maria Filimonova1Department of Microbiology, Kazan (Volga Region) Federal University, Kremliovskaya Street 18, Kazan 420008, Tatarstan, RussiaDepartment of Microbiology, Kazan (Volga Region) Federal University, Kremliovskaya Street 18, Kazan 420008, Tatarstan, RussiaExamination of the effects of mononucleotides on Sma nuc endonuclease originated from Gram negative bacterium Serratia marcescens displayed that any mononucleotide produced by Sma nuc during hydrolysis of DNA or RNA may regulate the enzyme activity affecting the RNase activity without pronounced influence on the activity towards DNA. The type of carbohydrate residue in mononucleotides does not affect the regulation. In contrast, the effects depend on the type of bases in nucleotides. AMP or dAMP was classified as a competitive inhibitor of partial type. GMP, UMP, and CMP were found to be uncompetitive inhibitors that suggest a specific site(s) for the nucleotide(s) binding in Sma nuc endonuclease.http://dx.doi.org/10.1100/2012/454176
collection DOAJ
language English
format Article
sources DOAJ
author Julia Romanova
Maria Filimonova
spellingShingle Julia Romanova
Maria Filimonova
The Effects of Addition of Mononucleotides on Sma nuc Endonuclease Activity
The Scientific World Journal
author_facet Julia Romanova
Maria Filimonova
author_sort Julia Romanova
title The Effects of Addition of Mononucleotides on Sma nuc Endonuclease Activity
title_short The Effects of Addition of Mononucleotides on Sma nuc Endonuclease Activity
title_full The Effects of Addition of Mononucleotides on Sma nuc Endonuclease Activity
title_fullStr The Effects of Addition of Mononucleotides on Sma nuc Endonuclease Activity
title_full_unstemmed The Effects of Addition of Mononucleotides on Sma nuc Endonuclease Activity
title_sort effects of addition of mononucleotides on sma nuc endonuclease activity
publisher Hindawi Limited
series The Scientific World Journal
issn 1537-744X
publishDate 2012-01-01
description Examination of the effects of mononucleotides on Sma nuc endonuclease originated from Gram negative bacterium Serratia marcescens displayed that any mononucleotide produced by Sma nuc during hydrolysis of DNA or RNA may regulate the enzyme activity affecting the RNase activity without pronounced influence on the activity towards DNA. The type of carbohydrate residue in mononucleotides does not affect the regulation. In contrast, the effects depend on the type of bases in nucleotides. AMP or dAMP was classified as a competitive inhibitor of partial type. GMP, UMP, and CMP were found to be uncompetitive inhibitors that suggest a specific site(s) for the nucleotide(s) binding in Sma nuc endonuclease.
url http://dx.doi.org/10.1100/2012/454176
work_keys_str_mv AT juliaromanova theeffectsofadditionofmononucleotidesonsmanucendonucleaseactivity
AT mariafilimonova theeffectsofadditionofmononucleotidesonsmanucendonucleaseactivity
AT juliaromanova effectsofadditionofmononucleotidesonsmanucendonucleaseactivity
AT mariafilimonova effectsofadditionofmononucleotidesonsmanucendonucleaseactivity
_version_ 1725077169273569280

Similar Items