The effect of ultrasonic energy on the separation of cytoplasmic RNA fractions

Plant tissue -4 days old germs of Zea mays and Pisum sativum were subjected to ultrasonic energy using generator with a constant frequency of 800 kHz. RNA was isolated by Mieczyński’s modification of the method described by Georgiev. Preparations of RNA were separated by electrophoresis on the colou...

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Main Authors: D. Tyrawska-Spychałowa, Z. Adamczyk, B. Halicz
Format: Article
Language:English
Published: Polish Botanical Society 2015-01-01
Series:Acta Societatis Botanicorum Poloniae
Online Access:https://pbsociety.org.pl/journals/index.php/asbp/article/view/5122
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spelling doaj-91be67478b0e4a44bc498b8b65455cc02020-11-25T02:50:42ZengPolish Botanical SocietyActa Societatis Botanicorum Poloniae2083-94802015-01-0142116317010.5586/asbp.1973.0104324The effect of ultrasonic energy on the separation of cytoplasmic RNA fractionsD. Tyrawska-Spychałowa0Z. Adamczyk1B. Halicz2University of ŁódźUniversity of ŁódźUniversity of ŁódźPlant tissue -4 days old germs of Zea mays and Pisum sativum were subjected to ultrasonic energy using generator with a constant frequency of 800 kHz. RNA was isolated by Mieczyński’s modification of the method described by Georgiev. Preparations of RNA were separated by electrophoresis on the coloured agar-gel. Depending on the degree of ultrasonication changes in three fractions of RNA: A1 - 28 S, A2 - 18 S and A3 - 4 S were observedhttps://pbsociety.org.pl/journals/index.php/asbp/article/view/5122
collection DOAJ
language English
format Article
sources DOAJ
author D. Tyrawska-Spychałowa
Z. Adamczyk
B. Halicz
spellingShingle D. Tyrawska-Spychałowa
Z. Adamczyk
B. Halicz
The effect of ultrasonic energy on the separation of cytoplasmic RNA fractions
Acta Societatis Botanicorum Poloniae
author_facet D. Tyrawska-Spychałowa
Z. Adamczyk
B. Halicz
author_sort D. Tyrawska-Spychałowa
title The effect of ultrasonic energy on the separation of cytoplasmic RNA fractions
title_short The effect of ultrasonic energy on the separation of cytoplasmic RNA fractions
title_full The effect of ultrasonic energy on the separation of cytoplasmic RNA fractions
title_fullStr The effect of ultrasonic energy on the separation of cytoplasmic RNA fractions
title_full_unstemmed The effect of ultrasonic energy on the separation of cytoplasmic RNA fractions
title_sort effect of ultrasonic energy on the separation of cytoplasmic rna fractions
publisher Polish Botanical Society
series Acta Societatis Botanicorum Poloniae
issn 2083-9480
publishDate 2015-01-01
description Plant tissue -4 days old germs of Zea mays and Pisum sativum were subjected to ultrasonic energy using generator with a constant frequency of 800 kHz. RNA was isolated by Mieczyński’s modification of the method described by Georgiev. Preparations of RNA were separated by electrophoresis on the coloured agar-gel. Depending on the degree of ultrasonication changes in three fractions of RNA: A1 - 28 S, A2 - 18 S and A3 - 4 S were observed
url https://pbsociety.org.pl/journals/index.php/asbp/article/view/5122
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