The effect of ultrasonic energy on the separation of cytoplasmic RNA fractions
Plant tissue -4 days old germs of Zea mays and Pisum sativum were subjected to ultrasonic energy using generator with a constant frequency of 800 kHz. RNA was isolated by Mieczyński’s modification of the method described by Georgiev. Preparations of RNA were separated by electrophoresis on the colou...
Main Authors: | , , |
---|---|
Format: | Article |
Language: | English |
Published: |
Polish Botanical Society
2015-01-01
|
Series: | Acta Societatis Botanicorum Poloniae |
Online Access: | https://pbsociety.org.pl/journals/index.php/asbp/article/view/5122 |
id |
doaj-91be67478b0e4a44bc498b8b65455cc0 |
---|---|
record_format |
Article |
spelling |
doaj-91be67478b0e4a44bc498b8b65455cc02020-11-25T02:50:42ZengPolish Botanical SocietyActa Societatis Botanicorum Poloniae2083-94802015-01-0142116317010.5586/asbp.1973.0104324The effect of ultrasonic energy on the separation of cytoplasmic RNA fractionsD. Tyrawska-Spychałowa0Z. Adamczyk1B. Halicz2University of ŁódźUniversity of ŁódźUniversity of ŁódźPlant tissue -4 days old germs of Zea mays and Pisum sativum were subjected to ultrasonic energy using generator with a constant frequency of 800 kHz. RNA was isolated by Mieczyński’s modification of the method described by Georgiev. Preparations of RNA were separated by electrophoresis on the coloured agar-gel. Depending on the degree of ultrasonication changes in three fractions of RNA: A1 - 28 S, A2 - 18 S and A3 - 4 S were observedhttps://pbsociety.org.pl/journals/index.php/asbp/article/view/5122 |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
D. Tyrawska-Spychałowa Z. Adamczyk B. Halicz |
spellingShingle |
D. Tyrawska-Spychałowa Z. Adamczyk B. Halicz The effect of ultrasonic energy on the separation of cytoplasmic RNA fractions Acta Societatis Botanicorum Poloniae |
author_facet |
D. Tyrawska-Spychałowa Z. Adamczyk B. Halicz |
author_sort |
D. Tyrawska-Spychałowa |
title |
The effect of ultrasonic energy on the separation of cytoplasmic RNA fractions |
title_short |
The effect of ultrasonic energy on the separation of cytoplasmic RNA fractions |
title_full |
The effect of ultrasonic energy on the separation of cytoplasmic RNA fractions |
title_fullStr |
The effect of ultrasonic energy on the separation of cytoplasmic RNA fractions |
title_full_unstemmed |
The effect of ultrasonic energy on the separation of cytoplasmic RNA fractions |
title_sort |
effect of ultrasonic energy on the separation of cytoplasmic rna fractions |
publisher |
Polish Botanical Society |
series |
Acta Societatis Botanicorum Poloniae |
issn |
2083-9480 |
publishDate |
2015-01-01 |
description |
Plant tissue -4 days old germs of Zea mays and Pisum sativum were subjected to ultrasonic energy using generator with a constant frequency of 800 kHz. RNA was isolated by Mieczyński’s modification of the method described by Georgiev. Preparations of RNA were separated by electrophoresis on the coloured agar-gel. Depending on the degree of ultrasonication changes in three fractions of RNA: A1 - 28 S, A2 - 18 S and A3 - 4 S were observed |
url |
https://pbsociety.org.pl/journals/index.php/asbp/article/view/5122 |
work_keys_str_mv |
AT dtyrawskaspychałowa theeffectofultrasonicenergyontheseparationofcytoplasmicrnafractions AT zadamczyk theeffectofultrasonicenergyontheseparationofcytoplasmicrnafractions AT bhalicz theeffectofultrasonicenergyontheseparationofcytoplasmicrnafractions AT dtyrawskaspychałowa effectofultrasonicenergyontheseparationofcytoplasmicrnafractions AT zadamczyk effectofultrasonicenergyontheseparationofcytoplasmicrnafractions AT bhalicz effectofultrasonicenergyontheseparationofcytoplasmicrnafractions |
_version_ |
1724736925710942208 |