The effect of ultrasonic energy on the separation of cytoplasmic RNA fractions
Plant tissue -4 days old germs of Zea mays and Pisum sativum were subjected to ultrasonic energy using generator with a constant frequency of 800 kHz. RNA was isolated by Mieczyński’s modification of the method described by Georgiev. Preparations of RNA were separated by electrophoresis on the colou...
| Main Authors: | , , |
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| Format: | Article |
| Language: | English |
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Polish Botanical Society
2015-01-01
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| Series: | Acta Societatis Botanicorum Poloniae |
| Online Access: | https://pbsociety.org.pl/journals/index.php/asbp/article/view/5122 |
| Summary: | Plant tissue -4 days old germs of Zea mays and Pisum sativum were subjected to ultrasonic energy using generator with a constant frequency of 800 kHz. RNA was isolated by Mieczyński’s modification of the method described by Georgiev. Preparations of RNA were separated by electrophoresis on the coloured agar-gel. Depending on the degree of ultrasonication changes in three fractions of RNA: A1 - 28 S, A2 - 18 S and A3 - 4 S were observed |
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| ISSN: | 2083-9480 |