Cell Electrical Impedance as a Novel Approach for Studies on Senescence Not Based on Biomarkers

Senescence of cardiac myocytes is frequently associated with heart diseases. To analyze senescence in cardiac myocytes, a number of biomarkers have been isolated. However, due to the complex nature of senescence, multiple markers are required for a single assay to accurately depict complex physiolog...

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Main Authors:	Jung-Joon Cha, Yangkyu Park, Joho Yun, Hyeon Woo Kim, Chang-Ju Park, Giseok Kang, Minhyun Jung, Boryeong Pak, Suk-Won Jin, Jong-Hyun Lee
Format:	Article
Language:	English
Published:	Hindawi Limited 2016-01-01
Series:	BioMed Research International
Online Access:	http://dx.doi.org/10.1155/2016/8484217

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spelling	doaj-91bb163412ab4d1186ad06dc679b3d222020-11-24T21:24:54ZengHindawi LimitedBioMed Research International2314-61332314-61412016-01-01201610.1155/2016/84842178484217Cell Electrical Impedance as a Novel Approach for Studies on Senescence Not Based on BiomarkersJung-Joon Cha0Yangkyu Park1Joho Yun2Hyeon Woo Kim3Chang-Ju Park4Giseok Kang5Minhyun Jung6Boryeong Pak7Suk-Won Jin8Jong-Hyun Lee9Department of Biomedical Science and Engineering, Gwangju Institute of Science and Technology (GIST), MEMS and Nano Systems Laboratory No. 317, Dasan Building, 123 Cheomdangwagi-ro, Buk-gu, Gwangju 61005, Republic of KoreaSchool of Mechanical Engineering, Gwangju Institute of Science and Technology (GIST), MEMS and Nano Systems Laboratory No. 317, Dasan Building, 123 Cheomdangwagi-ro, Buk-gu, Gwangju 61005, Republic of KoreaDepartment of Biomedical Science and Engineering, Gwangju Institute of Science and Technology (GIST), MEMS and Nano Systems Laboratory No. 317, Dasan Building, 123 Cheomdangwagi-ro, Buk-gu, Gwangju 61005, Republic of KoreaDepartment of Biomedical Science and Engineering, Gwangju Institute of Science and Technology (GIST), MEMS and Nano Systems Laboratory No. 317, Dasan Building, 123 Cheomdangwagi-ro, Buk-gu, Gwangju 61005, Republic of KoreaDepartment of Biomedical Science and Engineering, Gwangju Institute of Science and Technology (GIST), MEMS and Nano Systems Laboratory No. 317, Dasan Building, 123 Cheomdangwagi-ro, Buk-gu, Gwangju 61005, Republic of KoreaDepartment of Biomedical Science and Engineering, Gwangju Institute of Science and Technology (GIST), MEMS and Nano Systems Laboratory No. 317, Dasan Building, 123 Cheomdangwagi-ro, Buk-gu, Gwangju 61005, Republic of KoreaSchool of Mechanical Engineering, Gwangju Institute of Science and Technology (GIST), MEMS and Nano Systems Laboratory No. 317, Dasan Building, 123 Cheomdangwagi-ro, Buk-gu, Gwangju 61005, Republic of KoreaSchool of Life Sciences, Gwangju Institute of Science and Technology (GIST), Laboratory of Vascular Development No. 115, Life Science Building, 123 Cheomdangwagi-ro, Buk-gu, Gwangju 61005, Republic of KoreaSchool of Life Sciences, Gwangju Institute of Science and Technology (GIST), Laboratory of Vascular Development No. 115, Life Science Building, 123 Cheomdangwagi-ro, Buk-gu, Gwangju 61005, Republic of KoreaDepartment of Biomedical Science and Engineering, Gwangju Institute of Science and Technology (GIST), MEMS and Nano Systems Laboratory No. 317, Dasan Building, 123 Cheomdangwagi-ro, Buk-gu, Gwangju 61005, Republic of KoreaSenescence of cardiac myocytes is frequently associated with heart diseases. To analyze senescence in cardiac myocytes, a number of biomarkers have been isolated. However, due to the complex nature of senescence, multiple markers are required for a single assay to accurately depict complex physiological changes associated with senescence. In single cells, changes in both cytoplasm and cell membrane during senescence can affect the changes in electrical impedance. Based on this phenomenon, we developed MEDoS, a novel microelectrochemical impedance spectroscopy for diagnosis of senescence, which allows us to precisely measure quantitative changes in electrical properties of aging cells. Using cardiac myocytes isolated from 3-, 6-, and 18-month-old isogenic zebrafish, we examined the efficacy of MEDoS and showed that MEDoS can identify discernible changes in electrical impedance. Taken together, our data demonstrated that electrical impedance in cells at different ages is distinct with quantitative values; these results were comparable with previously reported ones. Therefore, we propose that MEDoS be used as a new biomarker-independent methodology to obtain quantitative data on the biological senescence status of individual cells.http://dx.doi.org/10.1155/2016/8484217
collection	DOAJ
language	English
format	Article
sources	DOAJ
author	Jung-Joon Cha Yangkyu Park Joho Yun Hyeon Woo Kim Chang-Ju Park Giseok Kang Minhyun Jung Boryeong Pak Suk-Won Jin Jong-Hyun Lee
spellingShingle	Jung-Joon Cha Yangkyu Park Joho Yun Hyeon Woo Kim Chang-Ju Park Giseok Kang Minhyun Jung Boryeong Pak Suk-Won Jin Jong-Hyun Lee Cell Electrical Impedance as a Novel Approach for Studies on Senescence Not Based on Biomarkers BioMed Research International
author_facet	Jung-Joon Cha Yangkyu Park Joho Yun Hyeon Woo Kim Chang-Ju Park Giseok Kang Minhyun Jung Boryeong Pak Suk-Won Jin Jong-Hyun Lee
author_sort	Jung-Joon Cha
title	Cell Electrical Impedance as a Novel Approach for Studies on Senescence Not Based on Biomarkers
title_short	Cell Electrical Impedance as a Novel Approach for Studies on Senescence Not Based on Biomarkers
title_full	Cell Electrical Impedance as a Novel Approach for Studies on Senescence Not Based on Biomarkers
title_fullStr	Cell Electrical Impedance as a Novel Approach for Studies on Senescence Not Based on Biomarkers
title_full_unstemmed	Cell Electrical Impedance as a Novel Approach for Studies on Senescence Not Based on Biomarkers
title_sort	cell electrical impedance as a novel approach for studies on senescence not based on biomarkers
publisher	Hindawi Limited
series	BioMed Research International
issn	2314-6133 2314-6141
publishDate	2016-01-01
description	Senescence of cardiac myocytes is frequently associated with heart diseases. To analyze senescence in cardiac myocytes, a number of biomarkers have been isolated. However, due to the complex nature of senescence, multiple markers are required for a single assay to accurately depict complex physiological changes associated with senescence. In single cells, changes in both cytoplasm and cell membrane during senescence can affect the changes in electrical impedance. Based on this phenomenon, we developed MEDoS, a novel microelectrochemical impedance spectroscopy for diagnosis of senescence, which allows us to precisely measure quantitative changes in electrical properties of aging cells. Using cardiac myocytes isolated from 3-, 6-, and 18-month-old isogenic zebrafish, we examined the efficacy of MEDoS and showed that MEDoS can identify discernible changes in electrical impedance. Taken together, our data demonstrated that electrical impedance in cells at different ages is distinct with quantitative values; these results were comparable with previously reported ones. Therefore, we propose that MEDoS be used as a new biomarker-independent methodology to obtain quantitative data on the biological senescence status of individual cells.
url	http://dx.doi.org/10.1155/2016/8484217
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Cell Electrical Impedance as a Novel Approach for Studies on Senescence Not Based on Biomarkers

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