Early Stages of we/we wal/wal Mouse Hair Morphogenesis: Light and Fluorescent Microscopy of the Whole-Mount Epidermis

In adult skin, hair follicles cyclically self-renew in a manner that recapitulates embryonic hair follicle morphogenesis. The most common pathology of hair in adults is alopecia, which is hair loss to different extent. There are a number of murine models of alopecia including spontaneous mutations....

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Main Authors: Alexandra Rippa, Olga Leonova, Vladimir Popenko, Andrey Vasiliev, Vasily Terskikh, Ekaterina Vorotelyak
Format: Article
Language:English
Published: Hindawi Limited 2014-01-01
Series:BioMed Research International
Online Access:http://dx.doi.org/10.1155/2014/856978
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spelling doaj-919fb42e188a4be4b1f49333d15045482020-11-24T20:55:03ZengHindawi LimitedBioMed Research International2314-61332314-61412014-01-01201410.1155/2014/856978856978Early Stages of we/we wal/wal Mouse Hair Morphogenesis: Light and Fluorescent Microscopy of the Whole-Mount EpidermisAlexandra Rippa0Olga Leonova1Vladimir Popenko2Andrey Vasiliev3Vasily Terskikh4Ekaterina Vorotelyak5Department of Biomedical Technologies, Pirogov Russian National Research Medical University, 1 Ostrovityanova, Moscow 117997, RussiaLaboratory of Cell Basics for Cancer Research, Engelhardt Institute of Molecular Biology, 32 Vavilov Street, Moscow 119991, RussiaLaboratory of Cell Basics for Cancer Research, Engelhardt Institute of Molecular Biology, 32 Vavilov Street, Moscow 119991, RussiaLaboratory of Cell Proliferation, N.K. Koltsov Institute of Developmental Biology, 26 Vavilov Street, Moscow 119334, RussiaLaboratory of Cell Proliferation, N.K. Koltsov Institute of Developmental Biology, 26 Vavilov Street, Moscow 119334, RussiaLaboratory of Cell Proliferation, N.K. Koltsov Institute of Developmental Biology, 26 Vavilov Street, Moscow 119334, RussiaIn adult skin, hair follicles cyclically self-renew in a manner that recapitulates embryonic hair follicle morphogenesis. The most common pathology of hair in adults is alopecia, which is hair loss to different extent. There are a number of murine models of alopecia including spontaneous mutations. In the present study, we worked with double homozygous we/we wal/wal mice which demonstrate symptoms closely resembling human alopecia. Using whole-mount preparations of epidermis of E18.5 embryos we show that hair follicle defects can be revealed as early as during embryonic morphogenesis in these mutants. The number of hair follicles was reduced almost 1.5-fold in mutant skin. The shape of the early stage small follicles was altered in mutant animals as compared to control ones. Additionally, follicles of mutant embryos were wider at the point of conjunction with interfollicular epidermis. We believe that the mutant mice studied represent a fascinating model to address the problem of hair loss. We demonstrated alterations in the morphogenesis of embryonic hair follicle in we/we wal/wal double homozygous mice developing alopecia postnatally. We suppose that incorrect morphogenesis of hair follicles during embryogenesis is closely related to alopecia in the adult life. Unveiling the mechanisms involved in altered embryogenesis may elucidate the pathogenesis of alopecia.http://dx.doi.org/10.1155/2014/856978
collection DOAJ
language English
format Article
sources DOAJ
author Alexandra Rippa
Olga Leonova
Vladimir Popenko
Andrey Vasiliev
Vasily Terskikh
Ekaterina Vorotelyak
spellingShingle Alexandra Rippa
Olga Leonova
Vladimir Popenko
Andrey Vasiliev
Vasily Terskikh
Ekaterina Vorotelyak
Early Stages of we/we wal/wal Mouse Hair Morphogenesis: Light and Fluorescent Microscopy of the Whole-Mount Epidermis
BioMed Research International
author_facet Alexandra Rippa
Olga Leonova
Vladimir Popenko
Andrey Vasiliev
Vasily Terskikh
Ekaterina Vorotelyak
author_sort Alexandra Rippa
title Early Stages of we/we wal/wal Mouse Hair Morphogenesis: Light and Fluorescent Microscopy of the Whole-Mount Epidermis
title_short Early Stages of we/we wal/wal Mouse Hair Morphogenesis: Light and Fluorescent Microscopy of the Whole-Mount Epidermis
title_full Early Stages of we/we wal/wal Mouse Hair Morphogenesis: Light and Fluorescent Microscopy of the Whole-Mount Epidermis
title_fullStr Early Stages of we/we wal/wal Mouse Hair Morphogenesis: Light and Fluorescent Microscopy of the Whole-Mount Epidermis
title_full_unstemmed Early Stages of we/we wal/wal Mouse Hair Morphogenesis: Light and Fluorescent Microscopy of the Whole-Mount Epidermis
title_sort early stages of we/we wal/wal mouse hair morphogenesis: light and fluorescent microscopy of the whole-mount epidermis
publisher Hindawi Limited
series BioMed Research International
issn 2314-6133
2314-6141
publishDate 2014-01-01
description In adult skin, hair follicles cyclically self-renew in a manner that recapitulates embryonic hair follicle morphogenesis. The most common pathology of hair in adults is alopecia, which is hair loss to different extent. There are a number of murine models of alopecia including spontaneous mutations. In the present study, we worked with double homozygous we/we wal/wal mice which demonstrate symptoms closely resembling human alopecia. Using whole-mount preparations of epidermis of E18.5 embryos we show that hair follicle defects can be revealed as early as during embryonic morphogenesis in these mutants. The number of hair follicles was reduced almost 1.5-fold in mutant skin. The shape of the early stage small follicles was altered in mutant animals as compared to control ones. Additionally, follicles of mutant embryos were wider at the point of conjunction with interfollicular epidermis. We believe that the mutant mice studied represent a fascinating model to address the problem of hair loss. We demonstrated alterations in the morphogenesis of embryonic hair follicle in we/we wal/wal double homozygous mice developing alopecia postnatally. We suppose that incorrect morphogenesis of hair follicles during embryogenesis is closely related to alopecia in the adult life. Unveiling the mechanisms involved in altered embryogenesis may elucidate the pathogenesis of alopecia.
url http://dx.doi.org/10.1155/2014/856978
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