Epidemiology of plasmid-mediated quinolone resistance in <it>salmonella enterica </it>serovar typhimurium isolates from food-producing animals in Japan

• Main Authors: Asai Tetsuo, Sato Chizuru, Masani Kaori, Usui Masaru, Ozawa Manao, Ogino Tomoe, Aoki Hiroshi, Sawada Takuo, Izumiya Hidemasa, Watanabe Haruo
• Format: Article
• Language: English
• Published: BMC 2010-12-01
• Series: Gut Pathogens
• Online Access: http://www.gutpathogens.com/content/2/1/17
• ISSN: 1757-4749

<p>Abstract</p> <p>A total of 225 isolates of <it>Salmonella enterica </it>serovar Typhimurium from food-producing animals collected between 2003 and 2007 were examined for the prevalence of plasmid-mediated quinolone resistance (PMQR) determinants, namely <it>qnrA, qnrB, qnrC, qnrD, qnrS, qepA </it>and <it>aac(6')Ib-cr</it>, in Japan. Two isolates (0.8%) of <it>S</it>. Typhimurium DT104 from different dairy cows on a single farm in 2006 and 2007 were found to have <it>qnrS1 </it>on a plasmid of approximately 9.6-kbp. None of the <it>S</it>. Typhimurium isolates had <it>qnrA, qnrB, qnrC, qnrD</it>, <it>qepA </it>and <it>acc(6')-Ib-cr</it>. Currently in Japan, the prevalence of the PMQR genes among <it>S</it>. Typhimurium isolates from food animals may remain low or restricted. The PFGE profile of two <it>S</it>. Typhimurium DT104 isolates without <it>qnrS1 </it>on the farm in 2005 had an identical PFGE profile to those of two <it>S</it>. Typhimurium DT104 isolates with <it>qnrS1</it>. The PFGE analysis suggested that the already existing <it>S</it>. Typhimurium DT104 on the farm fortuitously acquired the <it>qnrS1 </it>plasmid.</p>