A protocol for simultaneous in vivo juxtacellular electrophysiology and local pharmacological manipulation in mouse cortex

Summary: Here, we describe a protocol to simultaneously record and label single cortical neurons in vivo under local application of a chemical such as a receptor agonist. This protocol provides a useful tool to investigate how the chemical of interest affects the processing of sensory information by...

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Main Authors:	Ehsan Kheradpezhouh, Wricha Mishra, Ehsan Arabzadeh
Format:	Article
Language:	English
Published:	Elsevier 2021-03-01
Series:	STAR Protocols
Subjects:	Chemistry Analytical chemistry Chemical composition analysis
Online Access:	http://www.sciencedirect.com/science/article/pii/S2666166721000241

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spelling	doaj-914c010bccb34a3aabf38d47ab5238052021-03-22T12:53:11ZengElsevierSTAR Protocols2666-16672021-03-0121100317A protocol for simultaneous in vivo juxtacellular electrophysiology and local pharmacological manipulation in mouse cortexEhsan Kheradpezhouh0Wricha Mishra1Ehsan Arabzadeh2Eccles Institute of Neuroscience, John Curtin School of Medical Research, The Australian National University, Acton, Canberra, ACT 2601, Australia; The Australian Research Council Centre of Excellence for Integrative Brain Function, ACT Node, Caneberra, ACT 2601, Australia; Corresponding authorEccles Institute of Neuroscience, John Curtin School of Medical Research, The Australian National University, Acton, Canberra, ACT 2601, Australia; The Australian Research Council Centre of Excellence for Integrative Brain Function, ACT Node, Caneberra, ACT 2601, AustraliaEccles Institute of Neuroscience, John Curtin School of Medical Research, The Australian National University, Acton, Canberra, ACT 2601, Australia; The Australian Research Council Centre of Excellence for Integrative Brain Function, ACT Node, Caneberra, ACT 2601, AustraliaSummary: Here, we describe a protocol to simultaneously record and label single cortical neurons in vivo under local application of a chemical such as a receptor agonist. This protocol provides a useful tool to investigate how the chemical of interest affects the processing of sensory information by cortical neurons. The juxtacellular labeling allows identification of the cell type and morphology of the recorded neurons. We draw examples to show pharmacological modulations in encoding of vibrotactile stimuli in the mouse primary somatosensory cortex.For complete details on the use and execution of this protocol, please refer to Kheradpezhouh et al. (2020).http://www.sciencedirect.com/science/article/pii/S2666166721000241ChemistryAnalytical chemistryChemical composition analysis
collection	DOAJ
language	English
format	Article
sources	DOAJ
author	Ehsan Kheradpezhouh Wricha Mishra Ehsan Arabzadeh
spellingShingle	Ehsan Kheradpezhouh Wricha Mishra Ehsan Arabzadeh A protocol for simultaneous in vivo juxtacellular electrophysiology and local pharmacological manipulation in mouse cortex STAR Protocols Chemistry Analytical chemistry Chemical composition analysis
author_facet	Ehsan Kheradpezhouh Wricha Mishra Ehsan Arabzadeh
author_sort	Ehsan Kheradpezhouh
title	A protocol for simultaneous in vivo juxtacellular electrophysiology and local pharmacological manipulation in mouse cortex
title_short	A protocol for simultaneous in vivo juxtacellular electrophysiology and local pharmacological manipulation in mouse cortex
title_full	A protocol for simultaneous in vivo juxtacellular electrophysiology and local pharmacological manipulation in mouse cortex
title_fullStr	A protocol for simultaneous in vivo juxtacellular electrophysiology and local pharmacological manipulation in mouse cortex
title_full_unstemmed	A protocol for simultaneous in vivo juxtacellular electrophysiology and local pharmacological manipulation in mouse cortex
title_sort	protocol for simultaneous in vivo juxtacellular electrophysiology and local pharmacological manipulation in mouse cortex
publisher	Elsevier
series	STAR Protocols
issn	2666-1667
publishDate	2021-03-01
description	Summary: Here, we describe a protocol to simultaneously record and label single cortical neurons in vivo under local application of a chemical such as a receptor agonist. This protocol provides a useful tool to investigate how the chemical of interest affects the processing of sensory information by cortical neurons. The juxtacellular labeling allows identification of the cell type and morphology of the recorded neurons. We draw examples to show pharmacological modulations in encoding of vibrotactile stimuli in the mouse primary somatosensory cortex.For complete details on the use and execution of this protocol, please refer to Kheradpezhouh et al. (2020).
topic	Chemistry Analytical chemistry Chemical composition analysis
url	http://www.sciencedirect.com/science/article/pii/S2666166721000241
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A protocol for simultaneous in vivo juxtacellular electrophysiology and local pharmacological manipulation in mouse cortex

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