Performance of four modern whole genome amplification methods for copy number variant detection in single cells

Abstract Whole genome amplification (WGA) has become an invaluable tool to perform copy number variation (CNV) detection in single, or a limited number of cells. Unfortunately, current WGA methods introduce representation bias that limits the detection of small CNVs. New WGA methods have been introd...

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Main Authors:	Lieselot Deleye, Laurentijn Tilleman, Ann-Sophie Vander Plaetsen, Senne Cornelis, Dieter Deforce, Filip Van Nieuwerburgh
Format:	Article
Language:	English
Published:	Nature Publishing Group 2017-06-01
Series:	Scientific Reports
Online Access:	https://doi.org/10.1038/s41598-017-03711-y

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spelling	doaj-914070b89b484e859bf641aa7e3176362020-12-08T01:19:33ZengNature Publishing GroupScientific Reports2045-23222017-06-01711910.1038/s41598-017-03711-yPerformance of four modern whole genome amplification methods for copy number variant detection in single cellsLieselot Deleye0Laurentijn Tilleman1Ann-Sophie Vander Plaetsen2Senne Cornelis3Dieter Deforce4Filip Van Nieuwerburgh5Laboratory of Pharmaceutical Biotechnology, Ghent University, Ottergemsesteenweg 460Laboratory of Pharmaceutical Biotechnology, Ghent University, Ottergemsesteenweg 460Laboratory of Pharmaceutical Biotechnology, Ghent University, Ottergemsesteenweg 460Laboratory of Pharmaceutical Biotechnology, Ghent University, Ottergemsesteenweg 460Laboratory of Pharmaceutical Biotechnology, Ghent University, Ottergemsesteenweg 460Laboratory of Pharmaceutical Biotechnology, Ghent University, Ottergemsesteenweg 460Abstract Whole genome amplification (WGA) has become an invaluable tool to perform copy number variation (CNV) detection in single, or a limited number of cells. Unfortunately, current WGA methods introduce representation bias that limits the detection of small CNVs. New WGA methods have been introduced that might have the potential to reduce this bias. We compared the performance of PicoPLEX DNA-Seq (Picoseq), DOPlify, REPLI-g and Ampli-1 WGA for aneuploidy screening and copy number analysis using shallow whole genome massively parallel sequencing (MPS), starting from single or a limited number of cells. Although the four WGA methods perform differently, they are all suited for this application.https://doi.org/10.1038/s41598-017-03711-y
collection	DOAJ
language	English
format	Article
sources	DOAJ
author	Lieselot Deleye Laurentijn Tilleman Ann-Sophie Vander Plaetsen Senne Cornelis Dieter Deforce Filip Van Nieuwerburgh
spellingShingle	Lieselot Deleye Laurentijn Tilleman Ann-Sophie Vander Plaetsen Senne Cornelis Dieter Deforce Filip Van Nieuwerburgh Performance of four modern whole genome amplification methods for copy number variant detection in single cells Scientific Reports
author_facet	Lieselot Deleye Laurentijn Tilleman Ann-Sophie Vander Plaetsen Senne Cornelis Dieter Deforce Filip Van Nieuwerburgh
author_sort	Lieselot Deleye
title	Performance of four modern whole genome amplification methods for copy number variant detection in single cells
title_short	Performance of four modern whole genome amplification methods for copy number variant detection in single cells
title_full	Performance of four modern whole genome amplification methods for copy number variant detection in single cells
title_fullStr	Performance of four modern whole genome amplification methods for copy number variant detection in single cells
title_full_unstemmed	Performance of four modern whole genome amplification methods for copy number variant detection in single cells
title_sort	performance of four modern whole genome amplification methods for copy number variant detection in single cells
publisher	Nature Publishing Group
series	Scientific Reports
issn	2045-2322
publishDate	2017-06-01
description	Abstract Whole genome amplification (WGA) has become an invaluable tool to perform copy number variation (CNV) detection in single, or a limited number of cells. Unfortunately, current WGA methods introduce representation bias that limits the detection of small CNVs. New WGA methods have been introduced that might have the potential to reduce this bias. We compared the performance of PicoPLEX DNA-Seq (Picoseq), DOPlify, REPLI-g and Ampli-1 WGA for aneuploidy screening and copy number analysis using shallow whole genome massively parallel sequencing (MPS), starting from single or a limited number of cells. Although the four WGA methods perform differently, they are all suited for this application.
url	https://doi.org/10.1038/s41598-017-03711-y
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Performance of four modern whole genome amplification methods for copy number variant detection in single cells

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