| Summary: | Currently, researchers are looking for nanomaterials with peroxidase-like activity to replace natural peroxidase enzymes. For this purpose, WS<sub>2</sub> quantum dots (WS<sub>2</sub> QDs) were synthesized via a solvothermal method, which improved the mimetic behavior. The resulting WS<sub>2</sub> QDs with a size of 1⁻1.5 nm had a high fluorescence emission, dependent on the excitation wavelength. WS<sub>2</sub> QDs with uniform morphology showed a high catalytic effect in destroying H<sub>2</sub>O<sub>2</sub>. The peroxidase-like activity of synthesized nanostructures was studied in H<sub>2</sub>O<sub>2</sub> chemical and electrochemical reduction systems. The mimetic effect of WS<sub>2</sub> QDs was also shown in an H<sub>2</sub>O<sub>2</sub>⁻rhodamine B (RB) chemiluminescence system. For this aim, a stopped-flow chemiluminescence (CL) detection system was applied. Also, in order to confirm the peroxidase-like effect of quantum dots, colorimetry and electrochemical techniques were used. In the enzymatic reaction of glucose, H<sub>2</sub>O<sub>2</sub> is one of the products which can be determined. Under optimum conditions, H<sub>2</sub>O<sub>2</sub> can be detected in the concentration range of 0⁻1000 nmol·L<sup>−1</sup>, with a detection limit of 2.4 nmol·L<sup>−1</sup>. Using this CL assay, a linear relationship was obtained between the intensity of the CL emission and glucose concentration in the range of 0.01⁻30 nmol·L<sup>−1</sup>, with a limit of detection (3S) of 4.2 nmol·L<sup>−1</sup>.
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