Organogel nanoemulsion containing Nisin and D-limonene and its antimicrobial activity

• Main Authors: Weiya eBei, Yan eZhou, Xuya eXing, Mohamed Red eZahi, Yuan eLi, Qipeng eYuan, Hao eLiang
• Format: Article
• Language: English
• Published: Frontiers Media S.A. 2015-09-01
• Series: Frontiers in Microbiology
• Subjects: Food Preservation; Nisin; Antimicrobial activity; D-limonene; Organogel nanoemulsion
• Online Access: http://journal.frontiersin.org/Journal/10.3389/fmicb.2015.01010/full

The aim of this study was to investigate a novel delivery system containing D-limonene and nisin by food organogel-nanoemulsion and study its effect on the antimicrobial activity. Organogel-nanoemulsion containing with D-limonene and nisin or without nisin was prepared by a homogenization method. Factors that may affect the droplet size and stability of organogel-nanoemulsion such as pressure and surfactant to oil ratio were studied. The average droplet size decreased with pressure, and the organogel-nanoemulsion could achieve good stability at low surfactant to oil ratio. Positive effects and outstanding antimicrobial activities of organogel-nanoemulsion containing with D-limonene and nisin were confirmed by MICs comparison, growth curves of bacteria, scanning electron microscopy and determination of cell constituents’ release. Furthermore, the organogel-nanoemulsion applied as food preservative in milk also shown excellent antimicrobial performance. Overall, the research described in the current article show that organogel-nanoemulsion containing with D-limonene and nisin may be an effective antimicrobial system for the production and preservation of food.