Flow Injection Analysis with Microdialysis Probes Enable Minimally Invasive and Dynamic H<sub>2</sub>O<sub>2</sub> Measurements

Flow Injection Analysis with Microdialysis Probes Enable Minimally Invasive and Dynamic H<sub>2</sub>O<sub>2</sub> Measurements

This paper describes the optimization of a published flow injection analysis system coupled with microdialysis probes (MDP-FIA) for in-situ<i> </i>sampling and online measurements of hydrogen peroxide (H<sub>2</sub>O<sub>2</sub>). By modifying the commonly used Na...

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Bibliographic Details
Main Authors: Maria Moßhammer, Klaus Koren, Michael Kühl
Format: Article
Language:English
Published: MDPI AG 2018-12-01
Series:Proceedings
Subjects:
hydrogen peroxide, H2O2, flow injection analysis, FIA, optical chemical sensor, microdialysis probe, MDP, chemiluminescence, extracellular, MDP-FIA
Online Access:https://www.mdpi.com/2504-3900/2/13/992
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spelling doaj-910fbbb4868b4239b8c47f3b496a82b22020-11-25T02:46:37ZengMDPI AGProceedings2504-39002018-12-0121399210.3390/proceedings2130992proceedings2130992Flow Injection Analysis with Microdialysis Probes Enable Minimally Invasive and Dynamic H<sub>2</sub>O<sub>2</sub> MeasurementsMaria Moßhammer0Klaus Koren1Michael Kühl2Department of Biology, University of Copenhagen, 3000 Helsingør, DenmarkDepartment of Bioscience—Microbiology, University of Aarhus, 8000 Aarhus, DenmarkDepartment of Biology, University of Copenhagen, 3000 Helsingør, DenmarkThis paper describes the optimization of a published flow injection analysis system coupled with microdialysis probes (MDP-FIA) for in-situ<i> </i>sampling and online measurements of hydrogen peroxide (H<sub>2</sub>O<sub>2</sub>). By modifying the commonly used Na<sub>2</sub>CO<sub>3</sub> buffer by addition of EDTA and a changed order in reagent injection, interfering transition metals such as Fe(II) and Fe(III) are complexed and removed from the system without interfering with the chemiluminescent reaction of the used acridinium ester and H<sub>2</sub>O<sub>2</sub>. The system was then used to monitor changes in H<sub>2</sub>O<sub>2</sub> concentration upon microwaving seawater and filtered seawater in the presence and absence of agar.https://www.mdpi.com/2504-3900/2/13/992hydrogen peroxide, H2O2, flow injection analysis, FIA, optical chemical sensor, microdialysis probe, MDP, chemiluminescence, extracellular, MDP-FIA
collection DOAJ
language English
format Article
sources DOAJ
author Maria Moßhammer
Klaus Koren
Michael Kühl
spellingShingle Maria Moßhammer
Klaus Koren
Michael Kühl
Flow Injection Analysis with Microdialysis Probes Enable Minimally Invasive and Dynamic H<sub>2</sub>O<sub>2</sub> Measurements
Proceedings
hydrogen peroxide, H2O2, flow injection analysis, FIA, optical chemical sensor, microdialysis probe, MDP, chemiluminescence, extracellular, MDP-FIA
author_facet Maria Moßhammer
Klaus Koren
Michael Kühl
author_sort Maria Moßhammer
title Flow Injection Analysis with Microdialysis Probes Enable Minimally Invasive and Dynamic H<sub>2</sub>O<sub>2</sub> Measurements
title_short Flow Injection Analysis with Microdialysis Probes Enable Minimally Invasive and Dynamic H<sub>2</sub>O<sub>2</sub> Measurements
title_full Flow Injection Analysis with Microdialysis Probes Enable Minimally Invasive and Dynamic H<sub>2</sub>O<sub>2</sub> Measurements
title_fullStr Flow Injection Analysis with Microdialysis Probes Enable Minimally Invasive and Dynamic H<sub>2</sub>O<sub>2</sub> Measurements
title_full_unstemmed Flow Injection Analysis with Microdialysis Probes Enable Minimally Invasive and Dynamic H<sub>2</sub>O<sub>2</sub> Measurements
title_sort flow injection analysis with microdialysis probes enable minimally invasive and dynamic h<sub>2</sub>o<sub>2</sub> measurements
publisher MDPI AG
series Proceedings
issn 2504-3900
publishDate 2018-12-01
description This paper describes the optimization of a published flow injection analysis system coupled with microdialysis probes (MDP-FIA) for in-situ<i> </i>sampling and online measurements of hydrogen peroxide (H<sub>2</sub>O<sub>2</sub>). By modifying the commonly used Na<sub>2</sub>CO<sub>3</sub> buffer by addition of EDTA and a changed order in reagent injection, interfering transition metals such as Fe(II) and Fe(III) are complexed and removed from the system without interfering with the chemiluminescent reaction of the used acridinium ester and H<sub>2</sub>O<sub>2</sub>. The system was then used to monitor changes in H<sub>2</sub>O<sub>2</sub> concentration upon microwaving seawater and filtered seawater in the presence and absence of agar.
topic hydrogen peroxide, H2O2, flow injection analysis, FIA, optical chemical sensor, microdialysis probe, MDP, chemiluminescence, extracellular, MDP-FIA
url https://www.mdpi.com/2504-3900/2/13/992
work_keys_str_mv AT mariamoßhammer flowinjectionanalysiswithmicrodialysisprobesenableminimallyinvasiveanddynamichsub2subosub2submeasurements
AT klauskoren flowinjectionanalysiswithmicrodialysisprobesenableminimallyinvasiveanddynamichsub2subosub2submeasurements
AT michaelkuhl flowinjectionanalysiswithmicrodialysisprobesenableminimallyinvasiveanddynamichsub2subosub2submeasurements
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