Flow Injection Analysis with Microdialysis Probes Enable Minimally Invasive and Dynamic H₂O₂ Measurements

• Main Authors: Maria Moßhammer, Klaus Koren, Michael Kühl
• Format: Article
• Language: English
• Published: MDPI AG 2018-12-01
• Series: Proceedings
• Subjects: hydrogen peroxide, H2O2, flow injection analysis, FIA, optical chemical sensor, microdialysis probe, MDP, chemiluminescence, extracellular, MDP-FIA
• Online Access: https://www.mdpi.com/2504-3900/2/13/992
• ISSN: 2504-3900

This paper describes the optimization of a published flow injection analysis system coupled with microdialysis probes (MDP-FIA) for in-situ<i> </i>sampling and online measurements of hydrogen peroxide (H₂O₂). By modifying the commonly used Na₂CO₃ buffer by addition of EDTA and a changed order in reagent injection, interfering transition metals such as Fe(II) and Fe(III) are complexed and removed from the system without interfering with the chemiluminescent reaction of the used acridinium ester and H₂O₂. The system was then used to monitor changes in H₂O₂ concentration upon microwaving seawater and filtered seawater in the presence and absence of agar.