Multistable and dynamic CRISPRi-based synthetic circuits

Synthetic circuits based on CRISPRi have not achieved multistable and dynamic behaviors. Here the authors build an oscillator, a toggle switch and an incoherent feed-forward loop using CRISPRi, and provide a mathematical model suggesting that unspecific binding in CRISPRi enables multistability.

Bibliographic Details
Main Authors:	Javier Santos-Moreno, Eve Tasiudi, Joerg Stelling, Yolanda Schaerli
Format:	Article
Language:	English
Published:	Nature Publishing Group 2020-06-01
Series:	Nature Communications
Online Access:	https://doi.org/10.1038/s41467-020-16574-1

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spelling	doaj-91079cbffa374430abcf3eb11b0660662021-06-06T11:15:45ZengNature Publishing GroupNature Communications2041-17232020-06-011111810.1038/s41467-020-16574-1Multistable and dynamic CRISPRi-based synthetic circuitsJavier Santos-Moreno0Eve Tasiudi1Joerg Stelling2Yolanda Schaerli3Department of Fundamental Microbiology, University of LausanneDepartment of Biosystems Science and Engineering, ETH Zurich and SIB Swiss Institute of BioinformaticsDepartment of Biosystems Science and Engineering, ETH Zurich and SIB Swiss Institute of BioinformaticsDepartment of Fundamental Microbiology, University of LausanneSynthetic circuits based on CRISPRi have not achieved multistable and dynamic behaviors. Here the authors build an oscillator, a toggle switch and an incoherent feed-forward loop using CRISPRi, and provide a mathematical model suggesting that unspecific binding in CRISPRi enables multistability.https://doi.org/10.1038/s41467-020-16574-1
collection	DOAJ
language	English
format	Article
sources	DOAJ
author	Javier Santos-Moreno Eve Tasiudi Joerg Stelling Yolanda Schaerli
spellingShingle	Javier Santos-Moreno Eve Tasiudi Joerg Stelling Yolanda Schaerli Multistable and dynamic CRISPRi-based synthetic circuits Nature Communications
author_facet	Javier Santos-Moreno Eve Tasiudi Joerg Stelling Yolanda Schaerli
author_sort	Javier Santos-Moreno
title	Multistable and dynamic CRISPRi-based synthetic circuits
title_short	Multistable and dynamic CRISPRi-based synthetic circuits
title_full	Multistable and dynamic CRISPRi-based synthetic circuits
title_fullStr	Multistable and dynamic CRISPRi-based synthetic circuits
title_full_unstemmed	Multistable and dynamic CRISPRi-based synthetic circuits
title_sort	multistable and dynamic crispri-based synthetic circuits
publisher	Nature Publishing Group
series	Nature Communications
issn	2041-1723
publishDate	2020-06-01
description	Synthetic circuits based on CRISPRi have not achieved multistable and dynamic behaviors. Here the authors build an oscillator, a toggle switch and an incoherent feed-forward loop using CRISPRi, and provide a mathematical model suggesting that unspecific binding in CRISPRi enables multistability.
url	https://doi.org/10.1038/s41467-020-16574-1
work_keys_str_mv	AT javiersantosmoreno multistableanddynamiccrispribasedsyntheticcircuits AT evetasiudi multistableanddynamiccrispribasedsyntheticcircuits AT joergstelling multistableanddynamiccrispribasedsyntheticcircuits AT yolandaschaerli multistableanddynamiccrispribasedsyntheticcircuits
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Multistable and dynamic CRISPRi-based synthetic circuits

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