Effectiveness of double dose in single donor platelet preparation by the automated blood cell separators in continuous and intermittent flow system

• Main Authors: Warangkana Soloslikit, Pichet Vienghok
• Format: Article
• Language: English
• Published: Chaing Mai University 2016-09-01
• Series: Journal of Associated Medical Sciences
• Subjects: Single donor platelet; double dose; pre-count platelets; post-count platelets; platelet yield
• Online Access: https://www.tci-thaijo.org/index.php/bulletinAMS/article/view/69254
• ISSN: 2539-6056; 2539-6056

Background: To prepare single donor platelet (SDP) with good quality and sufficient quantity, beside safety to donors and patients, efficient and less costly automate blood cell separators also be considered.Objectives: To study the double-dose SDP products prepared from two automated blood cell separators having different principles. To compare platelet products in donors before, immediately after donation, and two weeks after donation. To evaluate change of average cost after double dose SDP preparation was implemented.Materials and methods: Semi-randomized study was performed in double-dose platelet donor (2 groups of 45 donors) in Blood Bank Unit, Phare Hospital during March to September 2015. Two automated blood cell separators, continuous flow: Trima Accel®  and intermittent flow: Haemonetics MCS plus /UPP® systems were compared in terms of platelet products, pre/post-count platelets and hematologic values before,  immediately, and two weeks after donation. Operation and efficiency of machine as well as average cost per dose of SDP preparation in 2013–2015 were also studied. Result: Quality and yield of platelet products obtained from Trima Accel® were significantly greater than those from Haemonetics MCS plus/UPP®; volume 444.0±41.5 mL, platelet yield 6.2±0.6x1011 cells, platelet 11.4±1.2 units for Trima Accel® and volume 385.1±87.1 mL, platelet yield 5.5±0.7x1011 cells, platelet 9.6±2.2 units for Haemonetics MCS plus/UPP® (p<0.001). After two weeks of donation, donors using Trima Accel® had significantly higher post-count platelet than those of Haemonetics MCS plus/UPP®. Collection efficiency of Trima Accel® was significantly higher than Haemonetics MCS plus/UPP® (p=0.042). Processing time per procedure of Trima Accel® and Haemonetics MCS plus/UPP® was averagely 82.3±13.7 minutes and  85.6±21.6 minutes, respectively, with no statistical significance. In 2015, cost per dose of SDP preparation for patients in Phrae Hospital decreased to 24.7% after increasing double dose SDP products to 34.8%.                                          Conclusion: Platelet yield products from Trima Accel® was superior to those from Haemonetics MCS plus/UPP®. After two weeks, double dose SDP donors donated by Trima Accel® had increased post-count platelets, suggesting that repeat donation can be done before four weeks. Both blood cell separators has no difference in processing time per procedure and double dose SDP preparation decreased cost of management.   Bull Chiang Mai Assoc Med Sci 2016; 49(2): 400-411. Doi: 10.14456/jams.2016.28