Development of Silica-Based Biodegradable Submicrometric Carriers and Investigating Their Characteristics as In Vitro Delivery Vehicles

Development of Silica-Based Biodegradable Submicrometric Carriers and Investigating Their Characteristics as In Vitro Delivery Vehicles

Nanostructured silica (SiO<sub>2</sub>)-based materials are attractive carriers for the delivery of bioactive compounds into cells. In this study, we developed hollow submicrometric particles composed of SiO<sub>2</sub> capsules that were separately loaded with various bioact...

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Main Authors: Mikhail V. Zyuzin, Dingcheng Zhu, Wolfgang J. Parak, Neus Feliu, Alberto Escudero
Format: Article
Language:English
Published: MDPI AG 2020-10-01
Series:International Journal of Molecular Sciences
Subjects:
silica capsules
gene delivery
endo/lysosomal escape
transfection
DNA
Online Access:https://www.mdpi.com/1422-0067/21/20/7563
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spelling doaj-90fccce0740a428388de7de813ff2a692020-11-25T03:55:10ZengMDPI AGInternational Journal of Molecular Sciences1661-65961422-00672020-10-01217563756310.3390/ijms21207563Development of Silica-Based Biodegradable Submicrometric Carriers and Investigating Their Characteristics as In Vitro Delivery VehiclesMikhail V. Zyuzin0Dingcheng Zhu1Wolfgang J. Parak2Neus Feliu3Alberto Escudero4Department of Physics and Engineering, ITMO University, Lomonosova 9, 191002 St. Petersburg, RussiaCenter for Hybrid Nanostructures (CHyN), Universität Hamburg, 22607 Hamburg, GermanyCenter for Hybrid Nanostructures (CHyN), Universität Hamburg, 22607 Hamburg, GermanyCenter for Hybrid Nanostructures (CHyN), Universität Hamburg, 22607 Hamburg, GermanyDepartamento de Química Inorgánica. Facultad de Química, Universidad de Sevilla, Calle Profesor García González 1, E–41012 Seville, SpainNanostructured silica (SiO<sub>2</sub>)-based materials are attractive carriers for the delivery of bioactive compounds into cells. In this study, we developed hollow submicrometric particles composed of SiO<sub>2</sub> capsules that were separately loaded with various bioactive molecules such as dextran, proteins, and nucleic acids. The structural characterization of the reported carriers was conducted using transmission and scanning electron microscopies (TEM/SEM), confocal laser scanning microscopy (CLSM), and dynamic light scattering (DLS). Moreover, the interaction of the developed carriers with cell lines was studied using standard viability, proliferation, and uptake assays. The submicrometric SiO<sub>2</sub>-based capsules loaded with DNA plasmid encoding green fluorescence proteins (GFP) were used to transfect cell lines. The obtained results were compared with studies made with similar capsules composed of polymers and show that SiO<sub>2</sub>-based capsules provide better transfection rates on the costs of higher toxicity.https://www.mdpi.com/1422-0067/21/20/7563silica capsulesgene deliveryendo/lysosomal escapetransfectionDNA
collection DOAJ
language English
format Article
sources DOAJ
author Mikhail V. Zyuzin
Dingcheng Zhu
Wolfgang J. Parak
Neus Feliu
Alberto Escudero
spellingShingle Mikhail V. Zyuzin
Dingcheng Zhu
Wolfgang J. Parak
Neus Feliu
Alberto Escudero
Development of Silica-Based Biodegradable Submicrometric Carriers and Investigating Their Characteristics as In Vitro Delivery Vehicles
International Journal of Molecular Sciences
silica capsules
gene delivery
endo/lysosomal escape
transfection
DNA
author_facet Mikhail V. Zyuzin
Dingcheng Zhu
Wolfgang J. Parak
Neus Feliu
Alberto Escudero
author_sort Mikhail V. Zyuzin
title Development of Silica-Based Biodegradable Submicrometric Carriers and Investigating Their Characteristics as In Vitro Delivery Vehicles
title_short Development of Silica-Based Biodegradable Submicrometric Carriers and Investigating Their Characteristics as In Vitro Delivery Vehicles
title_full Development of Silica-Based Biodegradable Submicrometric Carriers and Investigating Their Characteristics as In Vitro Delivery Vehicles
title_fullStr Development of Silica-Based Biodegradable Submicrometric Carriers and Investigating Their Characteristics as In Vitro Delivery Vehicles
title_full_unstemmed Development of Silica-Based Biodegradable Submicrometric Carriers and Investigating Their Characteristics as In Vitro Delivery Vehicles
title_sort development of silica-based biodegradable submicrometric carriers and investigating their characteristics as in vitro delivery vehicles
publisher MDPI AG
series International Journal of Molecular Sciences
issn 1661-6596
1422-0067
publishDate 2020-10-01
description Nanostructured silica (SiO<sub>2</sub>)-based materials are attractive carriers for the delivery of bioactive compounds into cells. In this study, we developed hollow submicrometric particles composed of SiO<sub>2</sub> capsules that were separately loaded with various bioactive molecules such as dextran, proteins, and nucleic acids. The structural characterization of the reported carriers was conducted using transmission and scanning electron microscopies (TEM/SEM), confocal laser scanning microscopy (CLSM), and dynamic light scattering (DLS). Moreover, the interaction of the developed carriers with cell lines was studied using standard viability, proliferation, and uptake assays. The submicrometric SiO<sub>2</sub>-based capsules loaded with DNA plasmid encoding green fluorescence proteins (GFP) were used to transfect cell lines. The obtained results were compared with studies made with similar capsules composed of polymers and show that SiO<sub>2</sub>-based capsules provide better transfection rates on the costs of higher toxicity.
topic silica capsules
gene delivery
endo/lysosomal escape
transfection
DNA
url https://www.mdpi.com/1422-0067/21/20/7563
work_keys_str_mv AT mikhailvzyuzin developmentofsilicabasedbiodegradablesubmicrometriccarriersandinvestigatingtheircharacteristicsasinvitrodeliveryvehicles
AT dingchengzhu developmentofsilicabasedbiodegradablesubmicrometriccarriersandinvestigatingtheircharacteristicsasinvitrodeliveryvehicles
AT wolfgangjparak developmentofsilicabasedbiodegradablesubmicrometriccarriersandinvestigatingtheircharacteristicsasinvitrodeliveryvehicles
AT neusfeliu developmentofsilicabasedbiodegradablesubmicrometriccarriersandinvestigatingtheircharacteristicsasinvitrodeliveryvehicles
AT albertoescudero developmentofsilicabasedbiodegradablesubmicrometriccarriersandinvestigatingtheircharacteristicsasinvitrodeliveryvehicles
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