Hypoxia and P. gingivalis Synergistically Induce HIF-1 and NF-κB Activation in PDL Cells and Periodontal Diseases

Periodontitis is characterized by deep periodontal pockets favoring the proliferation of anaerobic bacteria like Porphyromonas gingivalis (P. gingivalis), a periodontal pathogen frequently observed in patients suffering from periodontal inflammation. Therefore, the aim of the present study was to in...

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Main Authors: L. Gölz, S. Memmert, B. Rath-Deschner, A. Jäger, T. Appel, G. Baumgarten, W. Götz, S. Frede
Format: Article
Language:English
Published: Hindawi Limited 2015-01-01
Series:Mediators of Inflammation
Online Access:http://dx.doi.org/10.1155/2015/438085
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spelling doaj-90f5378322164191943caa678d0023162020-11-24T20:55:03ZengHindawi LimitedMediators of Inflammation0962-93511466-18612015-01-01201510.1155/2015/438085438085Hypoxia and P. gingivalis Synergistically Induce HIF-1 and NF-κB Activation in PDL Cells and Periodontal DiseasesL. Gölz0S. Memmert1B. Rath-Deschner2A. Jäger3T. Appel4G. Baumgarten5W. Götz6S. Frede7Department of Orthodontics, Dental Clinic, University Hospital of Bonn, Welschnonnenstraße 17, 53111 Bonn, GermanyDepartment of Orthodontics, Dental Clinic, University Hospital of Bonn, Welschnonnenstraße 17, 53111 Bonn, GermanyDepartment of Orthodontics, Dental Clinic, University Hospital of Bonn, Welschnonnenstraße 17, 53111 Bonn, GermanyDepartment of Orthodontics, Dental Clinic, University Hospital of Bonn, Welschnonnenstraße 17, 53111 Bonn, GermanyCenter of Dento-Maxillo-Facial Medicine, University Hospital of Bonn, Sigmund-Freud-Straße 25, 53105 Bonn, GermanyClinic of Anesthesiology and Intensive Care Medicine, University Hospital of Bonn, Welschnonnenstraße 17, 53111 Bonn, GermanyDepartment of Orthodontics, Dental Clinic, University Hospital of Bonn, Welschnonnenstraße 17, 53111 Bonn, GermanyClinic of Anesthesiology and Intensive Care Medicine, University Hospital of Bonn, Welschnonnenstraße 17, 53111 Bonn, GermanyPeriodontitis is characterized by deep periodontal pockets favoring the proliferation of anaerobic bacteria like Porphyromonas gingivalis (P. gingivalis), a periodontal pathogen frequently observed in patients suffering from periodontal inflammation. Therefore, the aim of the present study was to investigate the signaling pathways activated by lipopolysaccharide (LPS) of P. gingivalis (LPS-PG) and hypoxia in periodontal ligament (PDL) cells. The relevant transcription factors nuclear factor-kappa B (NF-κB) and hypoxia inducible factor-1 (HIF-1) were determined. In addition, we analyzed the expression of interleukin- (IL-) 1β, matrix metalloproteinase-1 (MMP-1), and vascular endothelial growth factor (VEGF) in PDL cells on mRNA and protein level. This was accomplished by immunohistochemistry of healthy and inflamed periodontal tissues. We detected time-dependent additive effects of LPS-PG and hypoxia on NF-κB and HIF-1α activation in PDL cells followed by an upregulation of IL-1β, MMP-1, and VEGF expression. Immunohistochemistry performed on tissue samples of gingivitis and periodontitis displayed an increase of NF-κB, HIF-1, and VEGF immunoreactivity in accordance with disease progression validating the importance of the in vitro results. To conclude, the present study underlines the significance of NF-κB and HIF-1α and their target genes VEGF, IL-1β, and MMP-1 in P. gingivalis and hypoxia induced periodontal inflammatory processes.http://dx.doi.org/10.1155/2015/438085
collection DOAJ
language English
format Article
sources DOAJ
author L. Gölz
S. Memmert
B. Rath-Deschner
A. Jäger
T. Appel
G. Baumgarten
W. Götz
S. Frede
spellingShingle L. Gölz
S. Memmert
B. Rath-Deschner
A. Jäger
T. Appel
G. Baumgarten
W. Götz
S. Frede
Hypoxia and P. gingivalis Synergistically Induce HIF-1 and NF-κB Activation in PDL Cells and Periodontal Diseases
Mediators of Inflammation
author_facet L. Gölz
S. Memmert
B. Rath-Deschner
A. Jäger
T. Appel
G. Baumgarten
W. Götz
S. Frede
author_sort L. Gölz
title Hypoxia and P. gingivalis Synergistically Induce HIF-1 and NF-κB Activation in PDL Cells and Periodontal Diseases
title_short Hypoxia and P. gingivalis Synergistically Induce HIF-1 and NF-κB Activation in PDL Cells and Periodontal Diseases
title_full Hypoxia and P. gingivalis Synergistically Induce HIF-1 and NF-κB Activation in PDL Cells and Periodontal Diseases
title_fullStr Hypoxia and P. gingivalis Synergistically Induce HIF-1 and NF-κB Activation in PDL Cells and Periodontal Diseases
title_full_unstemmed Hypoxia and P. gingivalis Synergistically Induce HIF-1 and NF-κB Activation in PDL Cells and Periodontal Diseases
title_sort hypoxia and p. gingivalis synergistically induce hif-1 and nf-κb activation in pdl cells and periodontal diseases
publisher Hindawi Limited
series Mediators of Inflammation
issn 0962-9351
1466-1861
publishDate 2015-01-01
description Periodontitis is characterized by deep periodontal pockets favoring the proliferation of anaerobic bacteria like Porphyromonas gingivalis (P. gingivalis), a periodontal pathogen frequently observed in patients suffering from periodontal inflammation. Therefore, the aim of the present study was to investigate the signaling pathways activated by lipopolysaccharide (LPS) of P. gingivalis (LPS-PG) and hypoxia in periodontal ligament (PDL) cells. The relevant transcription factors nuclear factor-kappa B (NF-κB) and hypoxia inducible factor-1 (HIF-1) were determined. In addition, we analyzed the expression of interleukin- (IL-) 1β, matrix metalloproteinase-1 (MMP-1), and vascular endothelial growth factor (VEGF) in PDL cells on mRNA and protein level. This was accomplished by immunohistochemistry of healthy and inflamed periodontal tissues. We detected time-dependent additive effects of LPS-PG and hypoxia on NF-κB and HIF-1α activation in PDL cells followed by an upregulation of IL-1β, MMP-1, and VEGF expression. Immunohistochemistry performed on tissue samples of gingivitis and periodontitis displayed an increase of NF-κB, HIF-1, and VEGF immunoreactivity in accordance with disease progression validating the importance of the in vitro results. To conclude, the present study underlines the significance of NF-κB and HIF-1α and their target genes VEGF, IL-1β, and MMP-1 in P. gingivalis and hypoxia induced periodontal inflammatory processes.
url http://dx.doi.org/10.1155/2015/438085
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