Possibilities of using a miniature fluorescence microscope

Possibilities of using a miniature fluorescence microscope

The article discusses an approach to fluorescence microscopy using single-photon miniature fluorescence microscopes. The use of miniscopes allows you to expand the possibilities of their usage and solve many problems related to the need of fixing experimental material, insufficient scanning speed, c...

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Main Authors: Erofeev Alexander, Gerasimov Evgeny, Barinov Dmitry, Bolsunovskaya Marina, Bezprozvanny Ilya
Format: Article
Language:English
Published: EDP Sciences 2020-01-01
Series:E3S Web of Conferences
Online Access:https://www.e3s-conferences.org/articles/e3sconf/pdf/2020/84/e3sconf_TPACEE2020_02005.pdf
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spelling doaj-90c0ba7523164506ba70d4a61307db282021-04-02T18:46:56ZengEDP SciencesE3S Web of Conferences2267-12422020-01-012240200510.1051/e3sconf/202022402005e3sconf_TPACEE2020_02005Possibilities of using a miniature fluorescence microscopeErofeev Alexander0Gerasimov Evgeny1Barinov Dmitry2Bolsunovskaya Marina3Bezprozvanny IlyaPeter The Great St. Petersburg Polytechnic UniversityPeter The Great St. Petersburg Polytechnic UniversityPeter The Great St. Petersburg Polytechnic UniversityPeter The Great St. Petersburg Polytechnic UniversityThe article discusses an approach to fluorescence microscopy using single-photon miniature fluorescence microscopes. The use of miniscopes allows you to expand the possibilities of their usage and solve many problems related to the need of fixing experimental material, insufficient scanning speed, complexity and high cost of usage. The authors developed a unique algorithm of correlations building based on cross-correlation of neuronal signals and implemented the opportunity to import experiment’s data in csv format. (as well as a utility for converting data from CNMF-E), which allows to import data from other sources. Special attention is paid to the methods of processing neural activity data: filtering the received frames from noise, compensating of image shifts and distortions.https://www.e3s-conferences.org/articles/e3sconf/pdf/2020/84/e3sconf_TPACEE2020_02005.pdf
collection DOAJ
language English
format Article
sources DOAJ
author Erofeev Alexander
Gerasimov Evgeny
Barinov Dmitry
Bolsunovskaya Marina
Bezprozvanny Ilya
spellingShingle Erofeev Alexander
Gerasimov Evgeny
Barinov Dmitry
Bolsunovskaya Marina
Bezprozvanny Ilya
Possibilities of using a miniature fluorescence microscope
E3S Web of Conferences
author_facet Erofeev Alexander
Gerasimov Evgeny
Barinov Dmitry
Bolsunovskaya Marina
Bezprozvanny Ilya
author_sort Erofeev Alexander
title Possibilities of using a miniature fluorescence microscope
title_short Possibilities of using a miniature fluorescence microscope
title_full Possibilities of using a miniature fluorescence microscope
title_fullStr Possibilities of using a miniature fluorescence microscope
title_full_unstemmed Possibilities of using a miniature fluorescence microscope
title_sort possibilities of using a miniature fluorescence microscope
publisher EDP Sciences
series E3S Web of Conferences
issn 2267-1242
publishDate 2020-01-01
description The article discusses an approach to fluorescence microscopy using single-photon miniature fluorescence microscopes. The use of miniscopes allows you to expand the possibilities of their usage and solve many problems related to the need of fixing experimental material, insufficient scanning speed, complexity and high cost of usage. The authors developed a unique algorithm of correlations building based on cross-correlation of neuronal signals and implemented the opportunity to import experiment’s data in csv format. (as well as a utility for converting data from CNMF-E), which allows to import data from other sources. Special attention is paid to the methods of processing neural activity data: filtering the received frames from noise, compensating of image shifts and distortions.
url https://www.e3s-conferences.org/articles/e3sconf/pdf/2020/84/e3sconf_TPACEE2020_02005.pdf
work_keys_str_mv AT erofeevalexander possibilitiesofusingaminiaturefluorescencemicroscope
AT gerasimovevgeny possibilitiesofusingaminiaturefluorescencemicroscope
AT barinovdmitry possibilitiesofusingaminiaturefluorescencemicroscope
AT bolsunovskayamarina possibilitiesofusingaminiaturefluorescencemicroscope
AT bezprozvannyilya possibilitiesofusingaminiaturefluorescencemicroscope
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