Accessing unexplored regions of sequence space in directed enzyme evolution via insertion/deletion mutagenesis

Insertions/Deletions (InDels) remain an untapped source of protein diversity in laboratory evolution. The method TRIAD generates libraries of random variants with short in-frame InDels using transposons, allowing a comparison of their evolutionary potential with widely-used point mutant libraries.

Bibliographic Details
Main Authors:	Stephane Emond, Maya Petek, Emily J. Kay, Brennen Heames, Sean R. A. Devenish, Nobuhiko Tokuriki, Florian Hollfelder
Format:	Article
Language:	English
Published:	Nature Publishing Group 2020-07-01
Series:	Nature Communications
Online Access:	https://doi.org/10.1038/s41467-020-17061-3

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spelling	doaj-90b00a5d4c434606bdb4fa1cf03f971e2021-07-11T11:44:26ZengNature Publishing GroupNature Communications2041-17232020-07-0111111410.1038/s41467-020-17061-3Accessing unexplored regions of sequence space in directed enzyme evolution via insertion/deletion mutagenesisStephane Emond0Maya Petek1Emily J. Kay2Brennen Heames3Sean R. A. Devenish4Nobuhiko Tokuriki5Florian Hollfelder6Department of Biochemistry, University of CambridgeDepartment of Biochemistry, University of CambridgeDepartment of Biochemistry, University of CambridgeDepartment of Biochemistry, University of CambridgeDepartment of Biochemistry, University of CambridgeMichael Smith Laboratories, University of British ColumbiaDepartment of Biochemistry, University of CambridgeInsertions/Deletions (InDels) remain an untapped source of protein diversity in laboratory evolution. The method TRIAD generates libraries of random variants with short in-frame InDels using transposons, allowing a comparison of their evolutionary potential with widely-used point mutant libraries.https://doi.org/10.1038/s41467-020-17061-3
collection	DOAJ
language	English
format	Article
sources	DOAJ
author	Stephane Emond Maya Petek Emily J. Kay Brennen Heames Sean R. A. Devenish Nobuhiko Tokuriki Florian Hollfelder
spellingShingle	Stephane Emond Maya Petek Emily J. Kay Brennen Heames Sean R. A. Devenish Nobuhiko Tokuriki Florian Hollfelder Accessing unexplored regions of sequence space in directed enzyme evolution via insertion/deletion mutagenesis Nature Communications
author_facet	Stephane Emond Maya Petek Emily J. Kay Brennen Heames Sean R. A. Devenish Nobuhiko Tokuriki Florian Hollfelder
author_sort	Stephane Emond
title	Accessing unexplored regions of sequence space in directed enzyme evolution via insertion/deletion mutagenesis
title_short	Accessing unexplored regions of sequence space in directed enzyme evolution via insertion/deletion mutagenesis
title_full	Accessing unexplored regions of sequence space in directed enzyme evolution via insertion/deletion mutagenesis
title_fullStr	Accessing unexplored regions of sequence space in directed enzyme evolution via insertion/deletion mutagenesis
title_full_unstemmed	Accessing unexplored regions of sequence space in directed enzyme evolution via insertion/deletion mutagenesis
title_sort	accessing unexplored regions of sequence space in directed enzyme evolution via insertion/deletion mutagenesis
publisher	Nature Publishing Group
series	Nature Communications
issn	2041-1723
publishDate	2020-07-01
description	Insertions/Deletions (InDels) remain an untapped source of protein diversity in laboratory evolution. The method TRIAD generates libraries of random variants with short in-frame InDels using transposons, allowing a comparison of their evolutionary potential with widely-used point mutant libraries.
url	https://doi.org/10.1038/s41467-020-17061-3
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Accessing unexplored regions of sequence space in directed enzyme evolution via insertion/deletion mutagenesis

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