Effect of a low cost diet on the cotton leaf worm, Spodoptera littoralis nucleopolyhedrosis virus pathogenicity and sterile insect technique

• Main Authors: Waheed A. A. Sayed, Alexandra El-Helaly, Zakia A. Jamal, Helmy El-Bendary
• Format: Article
• Language: English
• Published: SpringerOpen 2021-08-01
• Series: Egyptian Journal of Biological Pest Control
• Subjects: Lepidopteran-diet interaction; Gelling agents; Bioassay; Baculovirus infectivity; Male sterility
• Online Access: https://doi.org/10.1186/s41938-021-00464-9

Abstract Background Mass rearing cost of Spodoptera littoralis (Boisd.) (Lepidoptera: Noctuidae) is one of the critical methods for the successful utilization of Baculovirus pathogenicity and sterile insect technique (SIT). Effectiveness of both SIT and S. littoralis Nucleopolyhedrosis Virus (SpliNPV) was assessed in response to plant-based diet and substitution of agar with commercial sources of gelling components as feed. Results Pupal and adult recoveries produced by castor bean leaves were significantly high, followed by the agar-based diet. Moreover, larval durations were significantly prolonged for (starch + gelatin)-based diet than the other dietary diets. Obviously, SpliNPV pathogenicity against the larvae reared on (starch + gelatin)-based diet was 2.5 and 2 times higher than those reared on castor leaves and agar-based diet, respectively. Contrary to expectation, the sterility doses of male moths produced by castor leaves and agar-based diet were relatively similar. Conclusion The findings suggest that the lowest cost diets (starch + gelatin)-based diet could be used effectively for increasing the SpliNPV pathogenicity, while either castor leaves or agar-based diet could be considered as a promising choice for SIT program.