The Combination of Whole Cell Lipidomics Analysis and Single Cell Confocal Imaging of Fluidity and Micropolarity Provides Insight into Stress-Induced Lipid Turnover in Subcellular Organelles of Pancreatic Beta Cells

Modern omics techniques reveal molecular structures and cellular networks of tissues and cells in unprecedented detail. Recent advances in single cell analysis have further revolutionized all disciplines in cellular and molecular biology. These methods have also been employed in current investigatio...

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Main Authors:	Giuseppe Maulucci, Ofir Cohen, Bareket Daniel, Carla Ferreri, Shlomo Sasson
Format:	Article
Language:	English
Published:	MDPI AG 2019-10-01
Series:	Molecules
Subjects:	beta cells diabetes confocal microscopy lipidomics membrane fluidity maps cell micropolarity maps
Online Access:	https://www.mdpi.com/1420-3049/24/20/3742

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spelling	doaj-909a12b298f34457b595eedcde922cea2020-11-25T01:56:46ZengMDPI AGMolecules1420-30492019-10-012420374210.3390/molecules24203742molecules24203742The Combination of Whole Cell Lipidomics Analysis and Single Cell Confocal Imaging of Fluidity and Micropolarity Provides Insight into Stress-Induced Lipid Turnover in Subcellular Organelles of Pancreatic Beta CellsGiuseppe Maulucci0Ofir Cohen1Bareket Daniel2Carla Ferreri3Shlomo Sasson4Fondazione Policlinico Universitario A. Gemelli IRCSS, 00136 Rome, ItalyInstitute for Drug Research, Faculty of Medicine, The Hebrew University, 911210 Jerusalem, IsraelInstitute for Drug Research, Faculty of Medicine, The Hebrew University, 911210 Jerusalem, IsraelISOF, Consiglio Nazionale delle Ricerche, 40129 Bologna, ItalyInstitute for Drug Research, Faculty of Medicine, The Hebrew University, 911210 Jerusalem, IsraelModern omics techniques reveal molecular structures and cellular networks of tissues and cells in unprecedented detail. Recent advances in single cell analysis have further revolutionized all disciplines in cellular and molecular biology. These methods have also been employed in current investigations on the structure and function of insulin secreting beta cells under normal and pathological conditions that lead to an impaired glucose tolerance and type 2 diabetes. Proteomic and transcriptomic analyses have pointed to significant alterations in protein expression and function in beta cells exposed to diabetes like conditions (e.g., high glucose and/or saturated fatty acids levels). These nutritional overload stressful conditions are often defined as glucolipotoxic due to the progressive damage they cause to the cells. Our recent studies on the rat insulinoma-derived INS-1E beta cell line point to differential effects of such conditions in the phospholipid bilayers in beta cells. This review focuses on confocal microscopy-based detection of these profound alterations in the plasma membrane and membranes of insulin granules and lipid droplets in single beta cells under such nutritional load conditions.https://www.mdpi.com/1420-3049/24/20/3742beta cellsdiabetesconfocal microscopylipidomicsmembrane fluidity mapscell micropolarity maps
collection	DOAJ
language	English
format	Article
sources	DOAJ
author	Giuseppe Maulucci Ofir Cohen Bareket Daniel Carla Ferreri Shlomo Sasson
spellingShingle	Giuseppe Maulucci Ofir Cohen Bareket Daniel Carla Ferreri Shlomo Sasson The Combination of Whole Cell Lipidomics Analysis and Single Cell Confocal Imaging of Fluidity and Micropolarity Provides Insight into Stress-Induced Lipid Turnover in Subcellular Organelles of Pancreatic Beta Cells Molecules beta cells diabetes confocal microscopy lipidomics membrane fluidity maps cell micropolarity maps
author_facet	Giuseppe Maulucci Ofir Cohen Bareket Daniel Carla Ferreri Shlomo Sasson
author_sort	Giuseppe Maulucci
title	The Combination of Whole Cell Lipidomics Analysis and Single Cell Confocal Imaging of Fluidity and Micropolarity Provides Insight into Stress-Induced Lipid Turnover in Subcellular Organelles of Pancreatic Beta Cells
title_short	The Combination of Whole Cell Lipidomics Analysis and Single Cell Confocal Imaging of Fluidity and Micropolarity Provides Insight into Stress-Induced Lipid Turnover in Subcellular Organelles of Pancreatic Beta Cells
title_full	The Combination of Whole Cell Lipidomics Analysis and Single Cell Confocal Imaging of Fluidity and Micropolarity Provides Insight into Stress-Induced Lipid Turnover in Subcellular Organelles of Pancreatic Beta Cells
title_fullStr	The Combination of Whole Cell Lipidomics Analysis and Single Cell Confocal Imaging of Fluidity and Micropolarity Provides Insight into Stress-Induced Lipid Turnover in Subcellular Organelles of Pancreatic Beta Cells
title_full_unstemmed	The Combination of Whole Cell Lipidomics Analysis and Single Cell Confocal Imaging of Fluidity and Micropolarity Provides Insight into Stress-Induced Lipid Turnover in Subcellular Organelles of Pancreatic Beta Cells
title_sort	combination of whole cell lipidomics analysis and single cell confocal imaging of fluidity and micropolarity provides insight into stress-induced lipid turnover in subcellular organelles of pancreatic beta cells
publisher	MDPI AG
series	Molecules
issn	1420-3049
publishDate	2019-10-01
description	Modern omics techniques reveal molecular structures and cellular networks of tissues and cells in unprecedented detail. Recent advances in single cell analysis have further revolutionized all disciplines in cellular and molecular biology. These methods have also been employed in current investigations on the structure and function of insulin secreting beta cells under normal and pathological conditions that lead to an impaired glucose tolerance and type 2 diabetes. Proteomic and transcriptomic analyses have pointed to significant alterations in protein expression and function in beta cells exposed to diabetes like conditions (e.g., high glucose and/or saturated fatty acids levels). These nutritional overload stressful conditions are often defined as glucolipotoxic due to the progressive damage they cause to the cells. Our recent studies on the rat insulinoma-derived INS-1E beta cell line point to differential effects of such conditions in the phospholipid bilayers in beta cells. This review focuses on confocal microscopy-based detection of these profound alterations in the plasma membrane and membranes of insulin granules and lipid droplets in single beta cells under such nutritional load conditions.
topic	beta cells diabetes confocal microscopy lipidomics membrane fluidity maps cell micropolarity maps
url	https://www.mdpi.com/1420-3049/24/20/3742
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The Combination of Whole Cell Lipidomics Analysis and Single Cell Confocal Imaging of Fluidity and Micropolarity Provides Insight into Stress-Induced Lipid Turnover in Subcellular Organelles of Pancreatic Beta Cells

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