Linezolid Exerts Greater Bacterial Clearance but No Modification of Host Lung Gene Expression Profiling: A Mouse MRSA Pneumonia Model.

BACKGROUND:Linezolid (LZD) is beneficial to patients with MRSA pneumonia, but whether and how LZD influences global host lung immune responses at the mRNA level during MRSA-mediated pneumonia is still unknown. METHODS:A lethal mouse model of MRSA pneumonia mediated by USA300 was employed to study th...

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Main Authors: Jiwang Chen, Gang Feng, Yang Song, Juliane B Wardenburg, Simon Lin, Ichiro Inoshima, Michael Otto, Richard G Wunderink
Format: Article
Language:English
Published: Public Library of Science (PLoS) 2013-01-01
Series:PLoS ONE
Online Access:http://europepmc.org/articles/PMC3694879?pdf=render
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spelling doaj-90996291b8a14aefa69e418a74586c9d2020-11-25T02:12:47ZengPublic Library of Science (PLoS)PLoS ONE1932-62032013-01-0186e6799410.1371/journal.pone.0067994Linezolid Exerts Greater Bacterial Clearance but No Modification of Host Lung Gene Expression Profiling: A Mouse MRSA Pneumonia Model.Jiwang ChenGang FengYang SongJuliane B WardenburgSimon LinIchiro InoshimaMichael OttoRichard G WunderinkBACKGROUND:Linezolid (LZD) is beneficial to patients with MRSA pneumonia, but whether and how LZD influences global host lung immune responses at the mRNA level during MRSA-mediated pneumonia is still unknown. METHODS:A lethal mouse model of MRSA pneumonia mediated by USA300 was employed to study the influence of LZD on survival, while the sublethal mouse model was used to examine the effect of LZD on bacterial clearance and lung gene expression during MRSA pneumonia. LZD (100mg/kg/day, IP) was given to C57Bl6 mice for three days. On Day 1 and Day 3 post infection, bronchoalveolar lavage fluid (BALF) protein concentration and levels of cytokines including IL6, TNFα, IL1β, Interferon-γ and IL17 were measured. In the sublethal model, left lungs were used to determine bacterial clearance and right lungs for whole-genome transcriptional profiling of lung immune responses. RESULTS:LZD therapy significantly improved survival and bacterial clearance. It also significantly decreased BALF protein concentration and levels of cytokines including IL6, IL1β, Interferon-γ and IL17. No significant gene expression changes in the mouse lungs were associated with LZD therapy. CONCLUSION:LZD is beneficial to MRSA pneumonia, but it does not modulate host lung immune responses at the transcriptional level.http://europepmc.org/articles/PMC3694879?pdf=render
collection DOAJ
language English
format Article
sources DOAJ
author Jiwang Chen
Gang Feng
Yang Song
Juliane B Wardenburg
Simon Lin
Ichiro Inoshima
Michael Otto
Richard G Wunderink
spellingShingle Jiwang Chen
Gang Feng
Yang Song
Juliane B Wardenburg
Simon Lin
Ichiro Inoshima
Michael Otto
Richard G Wunderink
Linezolid Exerts Greater Bacterial Clearance but No Modification of Host Lung Gene Expression Profiling: A Mouse MRSA Pneumonia Model.
PLoS ONE
author_facet Jiwang Chen
Gang Feng
Yang Song
Juliane B Wardenburg
Simon Lin
Ichiro Inoshima
Michael Otto
Richard G Wunderink
author_sort Jiwang Chen
title Linezolid Exerts Greater Bacterial Clearance but No Modification of Host Lung Gene Expression Profiling: A Mouse MRSA Pneumonia Model.
title_short Linezolid Exerts Greater Bacterial Clearance but No Modification of Host Lung Gene Expression Profiling: A Mouse MRSA Pneumonia Model.
title_full Linezolid Exerts Greater Bacterial Clearance but No Modification of Host Lung Gene Expression Profiling: A Mouse MRSA Pneumonia Model.
title_fullStr Linezolid Exerts Greater Bacterial Clearance but No Modification of Host Lung Gene Expression Profiling: A Mouse MRSA Pneumonia Model.
title_full_unstemmed Linezolid Exerts Greater Bacterial Clearance but No Modification of Host Lung Gene Expression Profiling: A Mouse MRSA Pneumonia Model.
title_sort linezolid exerts greater bacterial clearance but no modification of host lung gene expression profiling: a mouse mrsa pneumonia model.
publisher Public Library of Science (PLoS)
series PLoS ONE
issn 1932-6203
publishDate 2013-01-01
description BACKGROUND:Linezolid (LZD) is beneficial to patients with MRSA pneumonia, but whether and how LZD influences global host lung immune responses at the mRNA level during MRSA-mediated pneumonia is still unknown. METHODS:A lethal mouse model of MRSA pneumonia mediated by USA300 was employed to study the influence of LZD on survival, while the sublethal mouse model was used to examine the effect of LZD on bacterial clearance and lung gene expression during MRSA pneumonia. LZD (100mg/kg/day, IP) was given to C57Bl6 mice for three days. On Day 1 and Day 3 post infection, bronchoalveolar lavage fluid (BALF) protein concentration and levels of cytokines including IL6, TNFα, IL1β, Interferon-γ and IL17 were measured. In the sublethal model, left lungs were used to determine bacterial clearance and right lungs for whole-genome transcriptional profiling of lung immune responses. RESULTS:LZD therapy significantly improved survival and bacterial clearance. It also significantly decreased BALF protein concentration and levels of cytokines including IL6, IL1β, Interferon-γ and IL17. No significant gene expression changes in the mouse lungs were associated with LZD therapy. CONCLUSION:LZD is beneficial to MRSA pneumonia, but it does not modulate host lung immune responses at the transcriptional level.
url http://europepmc.org/articles/PMC3694879?pdf=render
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