Simultaneous Absorbance and Fluorescence Measurements Using an Inlaid Microfluidic Approach
A novel microfluidic optical cell is presented that enables simultaneous measurement of both light absorbance and fluorescence on microlitre volumes of fluid. The chip design is based on an inlaid fabrication technique using clear and opaque poly(methyl methacrylate) or PMMA to create a 20.2 mm long...
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MDPI AG
2021-09-01
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| Series: | Sensors |
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| Online Access: | https://www.mdpi.com/1424-8220/21/18/6250 |
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doaj-90945494181d40129146d904c4a9b09e2021-09-26T01:23:53ZengMDPI AGSensors1424-82202021-09-01216250625010.3390/s21186250Simultaneous Absorbance and Fluorescence Measurements Using an Inlaid Microfluidic ApproachJoshua J. Creelman0Edward A. Luy1Gabryelle C. H. Beland2Colin Sonnichsen3Vincent J. Sieben4Department of Electrical and Computer Engineering, Dalhousie University, 1360 Barrington Street, Halifax, NS B3H 4R2, CanadaDartmouth Ocean Technologies Inc., 25 Parker Street, Dartmouth, NS B2Y 4T5, CanadaDepartment of Process Engineering and Applied Science, Dalhousie University, 5410 Spring Garden Road, Halifax, NS B3J 1B6, CanadaDepartment of Electrical and Computer Engineering, Dalhousie University, 1360 Barrington Street, Halifax, NS B3H 4R2, CanadaDepartment of Electrical and Computer Engineering, Dalhousie University, 1360 Barrington Street, Halifax, NS B3H 4R2, CanadaA novel microfluidic optical cell is presented that enables simultaneous measurement of both light absorbance and fluorescence on microlitre volumes of fluid. The chip design is based on an inlaid fabrication technique using clear and opaque poly(methyl methacrylate) or PMMA to create a 20.2 mm long optical cell. The inlaid approach allows fluid interrogation with minimal interference from external light over centimeter long path lengths. The performance of the optical cell is evaluated using a stable fluorescent dye: rhodamine B. Excellent linear relationships (R<sup>2</sup> > 0.99) are found for both absorbance and fluorescence over a 0.1–10 µM concentration range. Furthermore, the molar attenuation spectrum is accurately measured over the range 460–550 nm. The approach presented here is applicable to numerous colorimetric- or fluorescence-based assays and presents an important step in the development of multipurpose lab-on-chip sensors.https://www.mdpi.com/1424-8220/21/18/6250lab-on-chipmicrofluidicPMMArhodaminelight absorbancefluorescence |
| collection |
DOAJ |
| language |
English |
| format |
Article |
| sources |
DOAJ |
| author |
Joshua J. Creelman Edward A. Luy Gabryelle C. H. Beland Colin Sonnichsen Vincent J. Sieben |
| spellingShingle |
Joshua J. Creelman Edward A. Luy Gabryelle C. H. Beland Colin Sonnichsen Vincent J. Sieben Simultaneous Absorbance and Fluorescence Measurements Using an Inlaid Microfluidic Approach Sensors lab-on-chip microfluidic PMMA rhodamine light absorbance fluorescence |
| author_facet |
Joshua J. Creelman Edward A. Luy Gabryelle C. H. Beland Colin Sonnichsen Vincent J. Sieben |
| author_sort |
Joshua J. Creelman |
| title |
Simultaneous Absorbance and Fluorescence Measurements Using an Inlaid Microfluidic Approach |
| title_short |
Simultaneous Absorbance and Fluorescence Measurements Using an Inlaid Microfluidic Approach |
| title_full |
Simultaneous Absorbance and Fluorescence Measurements Using an Inlaid Microfluidic Approach |
| title_fullStr |
Simultaneous Absorbance and Fluorescence Measurements Using an Inlaid Microfluidic Approach |
| title_full_unstemmed |
Simultaneous Absorbance and Fluorescence Measurements Using an Inlaid Microfluidic Approach |
| title_sort |
simultaneous absorbance and fluorescence measurements using an inlaid microfluidic approach |
| publisher |
MDPI AG |
| series |
Sensors |
| issn |
1424-8220 |
| publishDate |
2021-09-01 |
| description |
A novel microfluidic optical cell is presented that enables simultaneous measurement of both light absorbance and fluorescence on microlitre volumes of fluid. The chip design is based on an inlaid fabrication technique using clear and opaque poly(methyl methacrylate) or PMMA to create a 20.2 mm long optical cell. The inlaid approach allows fluid interrogation with minimal interference from external light over centimeter long path lengths. The performance of the optical cell is evaluated using a stable fluorescent dye: rhodamine B. Excellent linear relationships (R<sup>2</sup> > 0.99) are found for both absorbance and fluorescence over a 0.1–10 µM concentration range. Furthermore, the molar attenuation spectrum is accurately measured over the range 460–550 nm. The approach presented here is applicable to numerous colorimetric- or fluorescence-based assays and presents an important step in the development of multipurpose lab-on-chip sensors. |
| topic |
lab-on-chip microfluidic PMMA rhodamine light absorbance fluorescence |
| url |
https://www.mdpi.com/1424-8220/21/18/6250 |
| work_keys_str_mv |
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