Transfection of Human Hematopoietic Stem Cells by a Gene Targeting Construct Containing the β-Globin Gene

Transfection of Human Hematopoietic Stem Cells by a Gene Targeting Construct Containing the β-Globin Gene

Objective: Replacment of CD133+ cell’s β globin gene by using a gene targeting constructcontaining the β globin gene and essential elements for homologous recombination.Materials and Methods: pFBGGT was amplified, then digested using the NheI and XhoIrestriction enzymes, and finally, a 13.3 kb band...

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Bibliographic Details
Main Authors: Sirous Zeinali, Bahman Zainali, Mohamadali Shokrgozar, Hossein Khanahmad, Seyedeh Soghra Moosavi, Esmat Kamali, Masood Soleimani, Mojhgan Shaikhpoor
Format: Article
Language:English
Published: Royan Institute (ACECR), Tehran 2010-01-01
Series:Cell Journal
Subjects:
Beta Thalassemia
Gene Targeting
Homologous Recombination
Gene Therapy
Online Access:http://celljournal.org/library/upload/article/SheikhPoor.pdf

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