Production of exopolysaccharide by strains of Lactobacillus plantarum YO175 and OF101 isolated from traditional fermented cereal beverage

Production of exopolysaccharide by strains of Lactobacillus plantarum YO175 and OF101 isolated from traditional fermented cereal beverage

Lactobacillus plantarum YO175 and OF101 isolates from Nigerian traditional fermented cereal gruel ‘ogi’, were investigated on the basis of their capability to produce exopolysaccharide (EPS) on sucrose modified deMan Rogosa Sharpe medium (mMRS). Functional groups analysis of the EPSs produced (EPS-Y...

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Main Authors: Adekemi Titilayo Adesulu-Dahunsi, Kumaraswamy Jeyaram, Abiodun Isiaka Sanni, Kolawole Banwo
Format: Article
Language:English
Published: PeerJ Inc. 2018-10-01
Series:PeerJ
Subjects:
Ogi
Lactobacillus plantarum
Exopolysaccharide
FT-IR
Antioxidant.
Online Access:https://peerj.com/articles/5326.pdf
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spelling doaj-9087857c366b42129df26b7e7c49fc7c2020-11-25T02:17:16ZengPeerJ Inc.PeerJ2167-83592018-10-016e532610.7717/peerj.5326Production of exopolysaccharide by strains of Lactobacillus plantarum YO175 and OF101 isolated from traditional fermented cereal beverageAdekemi Titilayo Adesulu-Dahunsi0Kumaraswamy Jeyaram1Abiodun Isiaka Sanni2Kolawole Banwo3Department of Microbiology, University of Ibadan, Ibadan, Oyo, NigeriaMicrobial Resource Division, Institute of Bioresources and Sustainable Development, Imphal, Manipur, IndiaDepartment of Microbiology, University of Ibadan, Ibadan, Oyo, NigeriaDepartment of Microbiology, University of Ibadan, Ibadan, Oyo, NigeriaLactobacillus plantarum YO175 and OF101 isolates from Nigerian traditional fermented cereal gruel ‘ogi’, were investigated on the basis of their capability to produce exopolysaccharide (EPS) on sucrose modified deMan Rogosa Sharpe medium (mMRS). Functional groups analysis of the EPSs produced (EPS-YO175 and EPS-OF101) by Fourier-transform infrared (FT-IR) spectroscopy revealed the presence of –OH, C=O and C–H groups. The chemical composition of EPS-YO175 and EPS-OF101 showed the presence of 87.1% and 80.62% carbohydrates and 1.21% and 1.47% protein. For maximum EPS yield, three significant factors were optimized using central composite design and response surface methodology, the predicted maximum EPS produced was 1.38 g/L and 2.19 g/L, while the experimental values were 1.36 g/L and 2.18 g/L for EPS-YO175 and EPS-OF101. The EPS samples showed strong antioxidant activities in-vitro. The scale-up of the production process of the EPS will find its potential application in food industries.https://peerj.com/articles/5326.pdfOgiLactobacillus plantarumExopolysaccharideFT-IRAntioxidant.
collection DOAJ
language English
format Article
sources DOAJ
author Adekemi Titilayo Adesulu-Dahunsi
Kumaraswamy Jeyaram
Abiodun Isiaka Sanni
Kolawole Banwo
spellingShingle Adekemi Titilayo Adesulu-Dahunsi
Kumaraswamy Jeyaram
Abiodun Isiaka Sanni
Kolawole Banwo
Production of exopolysaccharide by strains of Lactobacillus plantarum YO175 and OF101 isolated from traditional fermented cereal beverage
PeerJ
Ogi
Lactobacillus plantarum
Exopolysaccharide
FT-IR
Antioxidant.
author_facet Adekemi Titilayo Adesulu-Dahunsi
Kumaraswamy Jeyaram
Abiodun Isiaka Sanni
Kolawole Banwo
author_sort Adekemi Titilayo Adesulu-Dahunsi
title Production of exopolysaccharide by strains of Lactobacillus plantarum YO175 and OF101 isolated from traditional fermented cereal beverage
title_short Production of exopolysaccharide by strains of Lactobacillus plantarum YO175 and OF101 isolated from traditional fermented cereal beverage
title_full Production of exopolysaccharide by strains of Lactobacillus plantarum YO175 and OF101 isolated from traditional fermented cereal beverage
title_fullStr Production of exopolysaccharide by strains of Lactobacillus plantarum YO175 and OF101 isolated from traditional fermented cereal beverage
title_full_unstemmed Production of exopolysaccharide by strains of Lactobacillus plantarum YO175 and OF101 isolated from traditional fermented cereal beverage
title_sort production of exopolysaccharide by strains of lactobacillus plantarum yo175 and of101 isolated from traditional fermented cereal beverage
publisher PeerJ Inc.
series PeerJ
issn 2167-8359
publishDate 2018-10-01
description Lactobacillus plantarum YO175 and OF101 isolates from Nigerian traditional fermented cereal gruel ‘ogi’, were investigated on the basis of their capability to produce exopolysaccharide (EPS) on sucrose modified deMan Rogosa Sharpe medium (mMRS). Functional groups analysis of the EPSs produced (EPS-YO175 and EPS-OF101) by Fourier-transform infrared (FT-IR) spectroscopy revealed the presence of –OH, C=O and C–H groups. The chemical composition of EPS-YO175 and EPS-OF101 showed the presence of 87.1% and 80.62% carbohydrates and 1.21% and 1.47% protein. For maximum EPS yield, three significant factors were optimized using central composite design and response surface methodology, the predicted maximum EPS produced was 1.38 g/L and 2.19 g/L, while the experimental values were 1.36 g/L and 2.18 g/L for EPS-YO175 and EPS-OF101. The EPS samples showed strong antioxidant activities in-vitro. The scale-up of the production process of the EPS will find its potential application in food industries.
topic Ogi
Lactobacillus plantarum
Exopolysaccharide
FT-IR
Antioxidant.
url https://peerj.com/articles/5326.pdf
work_keys_str_mv AT adekemititilayoadesuludahunsi productionofexopolysaccharidebystrainsoflactobacillusplantarumyo175andof101isolatedfromtraditionalfermentedcerealbeverage
AT kumaraswamyjeyaram productionofexopolysaccharidebystrainsoflactobacillusplantarumyo175andof101isolatedfromtraditionalfermentedcerealbeverage
AT abiodunisiakasanni productionofexopolysaccharidebystrainsoflactobacillusplantarumyo175andof101isolatedfromtraditionalfermentedcerealbeverage
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