Establishment of a Human Gastric Cancer Xenograft Model in Immunocompetent Mice Using the Microcarrier-6

Gastric cancer is among the most common malignant tumors of the digestive tract. Establishing a robust and reliable animal model is the foundation for studying the pathogenesis of cancer. The present study established a mouse model of gastric carcinoma by inoculating immunocompetent mice with MKN45...

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Main Authors: Yanzhen Bi, Quanyi Wang, Yonghong Yang, Quanquan Wang, Kai Zhang, Xiaobei Zhang, William C. Cho, Zhenfeng Shu, Jiannan Li, Lili Liu, Chuanping Si, Feng Hong
Format: Article
Language:English
Published: Hindawi Limited 2020-01-01
Series:BioMed Research International
Online Access:http://dx.doi.org/10.1155/2020/1893434
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spelling doaj-9083fce2a0c04391b7d8ba8f4ef652182020-11-25T02:53:18ZengHindawi LimitedBioMed Research International2314-61332314-61412020-01-01202010.1155/2020/18934341893434Establishment of a Human Gastric Cancer Xenograft Model in Immunocompetent Mice Using the Microcarrier-6Yanzhen Bi0Quanyi Wang1Yonghong Yang2Quanquan Wang3Kai Zhang4Xiaobei Zhang5William C. Cho6Zhenfeng Shu7Jiannan Li8Lili Liu9Chuanping Si10Feng Hong11Difficult and Complicated Liver Diseases and Artificial Liver Center, Beijing Youan Hospital, Capital Medical University, Beijing, ChinaDepartment of Pathology, Affiliated Hospital of Jining Medical University, Jining, ChinaInstitute of Liver Diseases, Affiliated Hospital of Jining Medical University, Jining, ChinaDepartment of Neuromuscular Disease, The Third Hospital of Hebei Medical University, Shijiazhuang, ChinaDepartment of General Surgery, The Second Hospital of Jilin University, Changchun, ChinaInstitute of Liver Diseases, Affiliated Hospital of Jining Medical University, Jining, ChinaDepartment of Clinical Oncology, Queen Elizabeth Hospital, Hong Kong, ChinaShanghai Meifeng Biotechnology Co., Ltd, Shanghai, ChinaDepartment of General Surgery, The Second Hospital of Jilin University, Changchun, ChinaDepartment of Anorectal Surgery, Affiliated Hospital of Jining Medical University, Jining, ChinaInstitute of Immunology and Molecular Medicine, Jining Medical University, Jining, ChinaInstitute of Liver Diseases, Affiliated Hospital of Jining Medical University, Jining, ChinaGastric cancer is among the most common malignant tumors of the digestive tract. Establishing a robust and reliable animal model is the foundation for studying the pathogenesis of cancer. The present study established a mouse model of gastric carcinoma by inoculating immunocompetent mice with MKN45 cells using microcarrier. Sixty male C57BL/6 mice were randomly divided into three groups: a 2D group, an empty carrier group, and a 3D group, according to the coculture system of MKN45 and the microcarrier. The mouse models were established by hypodermic injection. Time to develop tumor, rate of tumor formation, and pathological features were observed in each group. In the 3D group, the tumorigenesis time was short, while the rate of tumor formation was high (75%). There was no detectable tumor formation in either the 2D or the empty carrier group. Both H&E and immunohistochemical staining of the tumor xenograft showed characteristic evidence of human gastric neoplasms. The present study successfully established a human gastric carcinoma model in immunocompetent mice, which provides a novel and valuable animal model for the cancer research and development of anticancer drugs.http://dx.doi.org/10.1155/2020/1893434
collection DOAJ
language English
format Article
sources DOAJ
author Yanzhen Bi
Quanyi Wang
Yonghong Yang
Quanquan Wang
Kai Zhang
Xiaobei Zhang
William C. Cho
Zhenfeng Shu
Jiannan Li
Lili Liu
Chuanping Si
Feng Hong
spellingShingle Yanzhen Bi
Quanyi Wang
Yonghong Yang
Quanquan Wang
Kai Zhang
Xiaobei Zhang
William C. Cho
Zhenfeng Shu
Jiannan Li
Lili Liu
Chuanping Si
Feng Hong
Establishment of a Human Gastric Cancer Xenograft Model in Immunocompetent Mice Using the Microcarrier-6
BioMed Research International
author_facet Yanzhen Bi
Quanyi Wang
Yonghong Yang
Quanquan Wang
Kai Zhang
Xiaobei Zhang
William C. Cho
Zhenfeng Shu
Jiannan Li
Lili Liu
Chuanping Si
Feng Hong
author_sort Yanzhen Bi
title Establishment of a Human Gastric Cancer Xenograft Model in Immunocompetent Mice Using the Microcarrier-6
title_short Establishment of a Human Gastric Cancer Xenograft Model in Immunocompetent Mice Using the Microcarrier-6
title_full Establishment of a Human Gastric Cancer Xenograft Model in Immunocompetent Mice Using the Microcarrier-6
title_fullStr Establishment of a Human Gastric Cancer Xenograft Model in Immunocompetent Mice Using the Microcarrier-6
title_full_unstemmed Establishment of a Human Gastric Cancer Xenograft Model in Immunocompetent Mice Using the Microcarrier-6
title_sort establishment of a human gastric cancer xenograft model in immunocompetent mice using the microcarrier-6
publisher Hindawi Limited
series BioMed Research International
issn 2314-6133
2314-6141
publishDate 2020-01-01
description Gastric cancer is among the most common malignant tumors of the digestive tract. Establishing a robust and reliable animal model is the foundation for studying the pathogenesis of cancer. The present study established a mouse model of gastric carcinoma by inoculating immunocompetent mice with MKN45 cells using microcarrier. Sixty male C57BL/6 mice were randomly divided into three groups: a 2D group, an empty carrier group, and a 3D group, according to the coculture system of MKN45 and the microcarrier. The mouse models were established by hypodermic injection. Time to develop tumor, rate of tumor formation, and pathological features were observed in each group. In the 3D group, the tumorigenesis time was short, while the rate of tumor formation was high (75%). There was no detectable tumor formation in either the 2D or the empty carrier group. Both H&E and immunohistochemical staining of the tumor xenograft showed characteristic evidence of human gastric neoplasms. The present study successfully established a human gastric carcinoma model in immunocompetent mice, which provides a novel and valuable animal model for the cancer research and development of anticancer drugs.
url http://dx.doi.org/10.1155/2020/1893434
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