Establishment of a Human Gastric Cancer Xenograft Model in Immunocompetent Mice Using the Microcarrier-6
Gastric cancer is among the most common malignant tumors of the digestive tract. Establishing a robust and reliable animal model is the foundation for studying the pathogenesis of cancer. The present study established a mouse model of gastric carcinoma by inoculating immunocompetent mice with MKN45...
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Online Access: | http://dx.doi.org/10.1155/2020/1893434 |
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doaj-9083fce2a0c04391b7d8ba8f4ef652182020-11-25T02:53:18ZengHindawi LimitedBioMed Research International2314-61332314-61412020-01-01202010.1155/2020/18934341893434Establishment of a Human Gastric Cancer Xenograft Model in Immunocompetent Mice Using the Microcarrier-6Yanzhen Bi0Quanyi Wang1Yonghong Yang2Quanquan Wang3Kai Zhang4Xiaobei Zhang5William C. Cho6Zhenfeng Shu7Jiannan Li8Lili Liu9Chuanping Si10Feng Hong11Difficult and Complicated Liver Diseases and Artificial Liver Center, Beijing Youan Hospital, Capital Medical University, Beijing, ChinaDepartment of Pathology, Affiliated Hospital of Jining Medical University, Jining, ChinaInstitute of Liver Diseases, Affiliated Hospital of Jining Medical University, Jining, ChinaDepartment of Neuromuscular Disease, The Third Hospital of Hebei Medical University, Shijiazhuang, ChinaDepartment of General Surgery, The Second Hospital of Jilin University, Changchun, ChinaInstitute of Liver Diseases, Affiliated Hospital of Jining Medical University, Jining, ChinaDepartment of Clinical Oncology, Queen Elizabeth Hospital, Hong Kong, ChinaShanghai Meifeng Biotechnology Co., Ltd, Shanghai, ChinaDepartment of General Surgery, The Second Hospital of Jilin University, Changchun, ChinaDepartment of Anorectal Surgery, Affiliated Hospital of Jining Medical University, Jining, ChinaInstitute of Immunology and Molecular Medicine, Jining Medical University, Jining, ChinaInstitute of Liver Diseases, Affiliated Hospital of Jining Medical University, Jining, ChinaGastric cancer is among the most common malignant tumors of the digestive tract. Establishing a robust and reliable animal model is the foundation for studying the pathogenesis of cancer. The present study established a mouse model of gastric carcinoma by inoculating immunocompetent mice with MKN45 cells using microcarrier. Sixty male C57BL/6 mice were randomly divided into three groups: a 2D group, an empty carrier group, and a 3D group, according to the coculture system of MKN45 and the microcarrier. The mouse models were established by hypodermic injection. Time to develop tumor, rate of tumor formation, and pathological features were observed in each group. In the 3D group, the tumorigenesis time was short, while the rate of tumor formation was high (75%). There was no detectable tumor formation in either the 2D or the empty carrier group. Both H&E and immunohistochemical staining of the tumor xenograft showed characteristic evidence of human gastric neoplasms. The present study successfully established a human gastric carcinoma model in immunocompetent mice, which provides a novel and valuable animal model for the cancer research and development of anticancer drugs.http://dx.doi.org/10.1155/2020/1893434 |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Yanzhen Bi Quanyi Wang Yonghong Yang Quanquan Wang Kai Zhang Xiaobei Zhang William C. Cho Zhenfeng Shu Jiannan Li Lili Liu Chuanping Si Feng Hong |
spellingShingle |
Yanzhen Bi Quanyi Wang Yonghong Yang Quanquan Wang Kai Zhang Xiaobei Zhang William C. Cho Zhenfeng Shu Jiannan Li Lili Liu Chuanping Si Feng Hong Establishment of a Human Gastric Cancer Xenograft Model in Immunocompetent Mice Using the Microcarrier-6 BioMed Research International |
author_facet |
Yanzhen Bi Quanyi Wang Yonghong Yang Quanquan Wang Kai Zhang Xiaobei Zhang William C. Cho Zhenfeng Shu Jiannan Li Lili Liu Chuanping Si Feng Hong |
author_sort |
Yanzhen Bi |
title |
Establishment of a Human Gastric Cancer Xenograft Model in Immunocompetent Mice Using the Microcarrier-6 |
title_short |
Establishment of a Human Gastric Cancer Xenograft Model in Immunocompetent Mice Using the Microcarrier-6 |
title_full |
Establishment of a Human Gastric Cancer Xenograft Model in Immunocompetent Mice Using the Microcarrier-6 |
title_fullStr |
Establishment of a Human Gastric Cancer Xenograft Model in Immunocompetent Mice Using the Microcarrier-6 |
title_full_unstemmed |
Establishment of a Human Gastric Cancer Xenograft Model in Immunocompetent Mice Using the Microcarrier-6 |
title_sort |
establishment of a human gastric cancer xenograft model in immunocompetent mice using the microcarrier-6 |
publisher |
Hindawi Limited |
series |
BioMed Research International |
issn |
2314-6133 2314-6141 |
publishDate |
2020-01-01 |
description |
Gastric cancer is among the most common malignant tumors of the digestive tract. Establishing a robust and reliable animal model is the foundation for studying the pathogenesis of cancer. The present study established a mouse model of gastric carcinoma by inoculating immunocompetent mice with MKN45 cells using microcarrier. Sixty male C57BL/6 mice were randomly divided into three groups: a 2D group, an empty carrier group, and a 3D group, according to the coculture system of MKN45 and the microcarrier. The mouse models were established by hypodermic injection. Time to develop tumor, rate of tumor formation, and pathological features were observed in each group. In the 3D group, the tumorigenesis time was short, while the rate of tumor formation was high (75%). There was no detectable tumor formation in either the 2D or the empty carrier group. Both H&E and immunohistochemical staining of the tumor xenograft showed characteristic evidence of human gastric neoplasms. The present study successfully established a human gastric carcinoma model in immunocompetent mice, which provides a novel and valuable animal model for the cancer research and development of anticancer drugs. |
url |
http://dx.doi.org/10.1155/2020/1893434 |
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