Effect of Aging on NK Cell Population and Their Proliferation at Ex Vivo Culture Condition

Age-associated changes in natural killer (NK) cell population, phenotype, and functions are directly attributed to the risk of several diseases and infections. It is predicted to be the major cause of the increase in mortality. Based on the surface density of CD56, NK cells are subdivided into two t...

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Main Authors: Sellamuthu Subbanna Gounder, Basri Johan Jeet Abdullah, Nur Ezzati Izyan Binti Mohd Radzuanb, Farah Dalila Binti Mohd Zain, Nurhidayah Bt Mohamad Sait, Corine Chua, Baskar Subramani
Format: Article
Language:English
Published: Hindawi Limited 2018-01-01
Series:Analytical Cellular Pathology
Online Access:http://dx.doi.org/10.1155/2018/7871814
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spelling doaj-907be32004d34458b251284fa331de362021-07-02T01:23:33ZengHindawi LimitedAnalytical Cellular Pathology2210-71772210-71852018-01-01201810.1155/2018/78718147871814Effect of Aging on NK Cell Population and Their Proliferation at Ex Vivo Culture ConditionSellamuthu Subbanna Gounder0Basri Johan Jeet Abdullah1Nur Ezzati Izyan Binti Mohd Radzuanb2Farah Dalila Binti Mohd Zain3Nurhidayah Bt Mohamad Sait4Corine Chua5Baskar Subramani6Nichi-Asia Life Science Sdn. Bhd., No. 57, Block F, Jalan Teknologi 3/9, Bistari De Kota, Kota Damansara, PJU 5, 47810 Petaling Jaya, Selangor, MalaysiaDepartment of Biomedical Imaging, University of Malaya, 50603 Kuala Lumpur, MalaysiaNichi-Asia Life Science Sdn. Bhd., No. 57, Block F, Jalan Teknologi 3/9, Bistari De Kota, Kota Damansara, PJU 5, 47810 Petaling Jaya, Selangor, MalaysiaNichi-Asia Life Science Sdn. Bhd., No. 57, Block F, Jalan Teknologi 3/9, Bistari De Kota, Kota Damansara, PJU 5, 47810 Petaling Jaya, Selangor, MalaysiaNichi-Asia Life Science Sdn. Bhd., No. 57, Block F, Jalan Teknologi 3/9, Bistari De Kota, Kota Damansara, PJU 5, 47810 Petaling Jaya, Selangor, MalaysiaDepartment of Biomedical Imaging, University of Malaya, 50603 Kuala Lumpur, MalaysiaNichi-Asia Life Science Sdn. Bhd., No. 57, Block F, Jalan Teknologi 3/9, Bistari De Kota, Kota Damansara, PJU 5, 47810 Petaling Jaya, Selangor, MalaysiaAge-associated changes in natural killer (NK) cell population, phenotype, and functions are directly attributed to the risk of several diseases and infections. It is predicted to be the major cause of the increase in mortality. Based on the surface density of CD56, NK cells are subdivided into two types, such as CD56bright and CD56dim cells, which represent cytokine production and cytotoxicity. In our study, we have examined the age-associated changes in the NK cell population and their subsets at different age groups of males and females (at a range from 41 to 80 years). We found that the total lymphocyte count significantly dropped upon aging in both genders. Although, the level of total immune cells also dropped on aging, and surprisingly the total NK cell population was remarkably increased with the majority of NK cells being CD56dim. Subsequently, we evaluated the proliferation potential of NK cells and our results showed that the NK cell proliferation ability declines with age. Overall, our findings prove that there is an increase in the circulating NK cell population upon aging. However, the proliferation rate upon aging declines when compared to the young age group (<41 yrs).http://dx.doi.org/10.1155/2018/7871814
collection DOAJ
language English
format Article
sources DOAJ
author Sellamuthu Subbanna Gounder
Basri Johan Jeet Abdullah
Nur Ezzati Izyan Binti Mohd Radzuanb
Farah Dalila Binti Mohd Zain
Nurhidayah Bt Mohamad Sait
Corine Chua
Baskar Subramani
spellingShingle Sellamuthu Subbanna Gounder
Basri Johan Jeet Abdullah
Nur Ezzati Izyan Binti Mohd Radzuanb
Farah Dalila Binti Mohd Zain
Nurhidayah Bt Mohamad Sait
Corine Chua
Baskar Subramani
Effect of Aging on NK Cell Population and Their Proliferation at Ex Vivo Culture Condition
Analytical Cellular Pathology
author_facet Sellamuthu Subbanna Gounder
Basri Johan Jeet Abdullah
Nur Ezzati Izyan Binti Mohd Radzuanb
Farah Dalila Binti Mohd Zain
Nurhidayah Bt Mohamad Sait
Corine Chua
Baskar Subramani
author_sort Sellamuthu Subbanna Gounder
title Effect of Aging on NK Cell Population and Their Proliferation at Ex Vivo Culture Condition
title_short Effect of Aging on NK Cell Population and Their Proliferation at Ex Vivo Culture Condition
title_full Effect of Aging on NK Cell Population and Their Proliferation at Ex Vivo Culture Condition
title_fullStr Effect of Aging on NK Cell Population and Their Proliferation at Ex Vivo Culture Condition
title_full_unstemmed Effect of Aging on NK Cell Population and Their Proliferation at Ex Vivo Culture Condition
title_sort effect of aging on nk cell population and their proliferation at ex vivo culture condition
publisher Hindawi Limited
series Analytical Cellular Pathology
issn 2210-7177
2210-7185
publishDate 2018-01-01
description Age-associated changes in natural killer (NK) cell population, phenotype, and functions are directly attributed to the risk of several diseases and infections. It is predicted to be the major cause of the increase in mortality. Based on the surface density of CD56, NK cells are subdivided into two types, such as CD56bright and CD56dim cells, which represent cytokine production and cytotoxicity. In our study, we have examined the age-associated changes in the NK cell population and their subsets at different age groups of males and females (at a range from 41 to 80 years). We found that the total lymphocyte count significantly dropped upon aging in both genders. Although, the level of total immune cells also dropped on aging, and surprisingly the total NK cell population was remarkably increased with the majority of NK cells being CD56dim. Subsequently, we evaluated the proliferation potential of NK cells and our results showed that the NK cell proliferation ability declines with age. Overall, our findings prove that there is an increase in the circulating NK cell population upon aging. However, the proliferation rate upon aging declines when compared to the young age group (<41 yrs).
url http://dx.doi.org/10.1155/2018/7871814
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