Effect of Aging on NK Cell Population and Their Proliferation at Ex Vivo Culture Condition
Age-associated changes in natural killer (NK) cell population, phenotype, and functions are directly attributed to the risk of several diseases and infections. It is predicted to be the major cause of the increase in mortality. Based on the surface density of CD56, NK cells are subdivided into two t...
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doaj-907be32004d34458b251284fa331de362021-07-02T01:23:33ZengHindawi LimitedAnalytical Cellular Pathology2210-71772210-71852018-01-01201810.1155/2018/78718147871814Effect of Aging on NK Cell Population and Their Proliferation at Ex Vivo Culture ConditionSellamuthu Subbanna Gounder0Basri Johan Jeet Abdullah1Nur Ezzati Izyan Binti Mohd Radzuanb2Farah Dalila Binti Mohd Zain3Nurhidayah Bt Mohamad Sait4Corine Chua5Baskar Subramani6Nichi-Asia Life Science Sdn. Bhd., No. 57, Block F, Jalan Teknologi 3/9, Bistari De Kota, Kota Damansara, PJU 5, 47810 Petaling Jaya, Selangor, MalaysiaDepartment of Biomedical Imaging, University of Malaya, 50603 Kuala Lumpur, MalaysiaNichi-Asia Life Science Sdn. Bhd., No. 57, Block F, Jalan Teknologi 3/9, Bistari De Kota, Kota Damansara, PJU 5, 47810 Petaling Jaya, Selangor, MalaysiaNichi-Asia Life Science Sdn. Bhd., No. 57, Block F, Jalan Teknologi 3/9, Bistari De Kota, Kota Damansara, PJU 5, 47810 Petaling Jaya, Selangor, MalaysiaNichi-Asia Life Science Sdn. Bhd., No. 57, Block F, Jalan Teknologi 3/9, Bistari De Kota, Kota Damansara, PJU 5, 47810 Petaling Jaya, Selangor, MalaysiaDepartment of Biomedical Imaging, University of Malaya, 50603 Kuala Lumpur, MalaysiaNichi-Asia Life Science Sdn. Bhd., No. 57, Block F, Jalan Teknologi 3/9, Bistari De Kota, Kota Damansara, PJU 5, 47810 Petaling Jaya, Selangor, MalaysiaAge-associated changes in natural killer (NK) cell population, phenotype, and functions are directly attributed to the risk of several diseases and infections. It is predicted to be the major cause of the increase in mortality. Based on the surface density of CD56, NK cells are subdivided into two types, such as CD56bright and CD56dim cells, which represent cytokine production and cytotoxicity. In our study, we have examined the age-associated changes in the NK cell population and their subsets at different age groups of males and females (at a range from 41 to 80 years). We found that the total lymphocyte count significantly dropped upon aging in both genders. Although, the level of total immune cells also dropped on aging, and surprisingly the total NK cell population was remarkably increased with the majority of NK cells being CD56dim. Subsequently, we evaluated the proliferation potential of NK cells and our results showed that the NK cell proliferation ability declines with age. Overall, our findings prove that there is an increase in the circulating NK cell population upon aging. However, the proliferation rate upon aging declines when compared to the young age group (<41 yrs).http://dx.doi.org/10.1155/2018/7871814 |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Sellamuthu Subbanna Gounder Basri Johan Jeet Abdullah Nur Ezzati Izyan Binti Mohd Radzuanb Farah Dalila Binti Mohd Zain Nurhidayah Bt Mohamad Sait Corine Chua Baskar Subramani |
spellingShingle |
Sellamuthu Subbanna Gounder Basri Johan Jeet Abdullah Nur Ezzati Izyan Binti Mohd Radzuanb Farah Dalila Binti Mohd Zain Nurhidayah Bt Mohamad Sait Corine Chua Baskar Subramani Effect of Aging on NK Cell Population and Their Proliferation at Ex Vivo Culture Condition Analytical Cellular Pathology |
author_facet |
Sellamuthu Subbanna Gounder Basri Johan Jeet Abdullah Nur Ezzati Izyan Binti Mohd Radzuanb Farah Dalila Binti Mohd Zain Nurhidayah Bt Mohamad Sait Corine Chua Baskar Subramani |
author_sort |
Sellamuthu Subbanna Gounder |
title |
Effect of Aging on NK Cell Population and Their Proliferation at Ex Vivo Culture Condition |
title_short |
Effect of Aging on NK Cell Population and Their Proliferation at Ex Vivo Culture Condition |
title_full |
Effect of Aging on NK Cell Population and Their Proliferation at Ex Vivo Culture Condition |
title_fullStr |
Effect of Aging on NK Cell Population and Their Proliferation at Ex Vivo Culture Condition |
title_full_unstemmed |
Effect of Aging on NK Cell Population and Their Proliferation at Ex Vivo Culture Condition |
title_sort |
effect of aging on nk cell population and their proliferation at ex vivo culture condition |
publisher |
Hindawi Limited |
series |
Analytical Cellular Pathology |
issn |
2210-7177 2210-7185 |
publishDate |
2018-01-01 |
description |
Age-associated changes in natural killer (NK) cell population, phenotype, and functions are directly attributed to the risk of several diseases and infections. It is predicted to be the major cause of the increase in mortality. Based on the surface density of CD56, NK cells are subdivided into two types, such as CD56bright and CD56dim cells, which represent cytokine production and cytotoxicity. In our study, we have examined the age-associated changes in the NK cell population and their subsets at different age groups of males and females (at a range from 41 to 80 years). We found that the total lymphocyte count significantly dropped upon aging in both genders. Although, the level of total immune cells also dropped on aging, and surprisingly the total NK cell population was remarkably increased with the majority of NK cells being CD56dim. Subsequently, we evaluated the proliferation potential of NK cells and our results showed that the NK cell proliferation ability declines with age. Overall, our findings prove that there is an increase in the circulating NK cell population upon aging. However, the proliferation rate upon aging declines when compared to the young age group (<41 yrs). |
url |
http://dx.doi.org/10.1155/2018/7871814 |
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