Supporting data for the characterization of PNA–DNA four-way junctions

Supporting data for the characterization of PNA–DNA four-way junctions

Holliday or DNA four-way junctions (4WJs) are cruciform/bent structures composed of four DNA duplexes. 4WJs are key intermediates in homologous genetic recombination and double-strand break repair. To investigate 4WJs in vitro, junctions are assembled using four asymmetric DNA strands. The presence...

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Main Authors: Douglas Iverson, Crystal Serrano, Ann Marie Brahan, Arik Shams, Filbert Totsingan, Anthony J. Bell Jr.
Format: Article
Language:English
Published: Elsevier 2015-12-01
Series:Data in Brief
Online Access:http://www.sciencedirect.com/science/article/pii/S2352340915002619
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spelling doaj-90716fb6328d496cb18880bfc38760612020-11-25T01:25:43ZengElsevierData in Brief2352-34092015-12-015C75676010.1016/j.dib.2015.10.013Supporting data for the characterization of PNA–DNA four-way junctionsDouglas Iverson0Crystal Serrano1Ann Marie Brahan2Arik Shams3Filbert Totsingan4Anthony J. Bell Jr.5Department of Chemistry and Biochemistry, The University of Southern Mississippi, Hattiesburg, MS, USADepartment of Chemistry and Biochemistry, The University of Southern Mississippi, Hattiesburg, MS, USADepartment of Chemistry and Biochemistry, The University of Southern Mississippi, Hattiesburg, MS, USADepartment of Chemistry and Biochemistry, The University of Southern Mississippi, Hattiesburg, MS, USADepartment of Chemistry, New York University, New York, NY, USADepartment of Chemistry and Biochemistry, The University of Southern Mississippi, Hattiesburg, MS, USAHolliday or DNA four-way junctions (4WJs) are cruciform/bent structures composed of four DNA duplexes. 4WJs are key intermediates in homologous genetic recombination and double-strand break repair. To investigate 4WJs in vitro, junctions are assembled using four asymmetric DNA strands. The presence of four asymmetric strands about the junction branch point eliminates branch migration, and effectively immobilizes the resulting 4WJ. The purpose of these experiments is to show that immobile 4WJs composed of DNA and peptide nucleic acids (PNAs) can be distinguished from contaminating labile nucleic acid structures. These data compare the electrophoretic mobility of hybrid PNA–DNA junctions vs. i) a classic immobile DNA 4WJ, J1 and ii) contaminating nucleic acid structures.http://www.sciencedirect.com/science/article/pii/S2352340915002619
collection DOAJ
language English
format Article
sources DOAJ
author Douglas Iverson
Crystal Serrano
Ann Marie Brahan
Arik Shams
Filbert Totsingan
Anthony J. Bell Jr.
spellingShingle Douglas Iverson
Crystal Serrano
Ann Marie Brahan
Arik Shams
Filbert Totsingan
Anthony J. Bell Jr.
Supporting data for the characterization of PNA–DNA four-way junctions
Data in Brief
author_facet Douglas Iverson
Crystal Serrano
Ann Marie Brahan
Arik Shams
Filbert Totsingan
Anthony J. Bell Jr.
author_sort Douglas Iverson
title Supporting data for the characterization of PNA–DNA four-way junctions
title_short Supporting data for the characterization of PNA–DNA four-way junctions
title_full Supporting data for the characterization of PNA–DNA four-way junctions
title_fullStr Supporting data for the characterization of PNA–DNA four-way junctions
title_full_unstemmed Supporting data for the characterization of PNA–DNA four-way junctions
title_sort supporting data for the characterization of pna–dna four-way junctions
publisher Elsevier
series Data in Brief
issn 2352-3409
publishDate 2015-12-01
description Holliday or DNA four-way junctions (4WJs) are cruciform/bent structures composed of four DNA duplexes. 4WJs are key intermediates in homologous genetic recombination and double-strand break repair. To investigate 4WJs in vitro, junctions are assembled using four asymmetric DNA strands. The presence of four asymmetric strands about the junction branch point eliminates branch migration, and effectively immobilizes the resulting 4WJ. The purpose of these experiments is to show that immobile 4WJs composed of DNA and peptide nucleic acids (PNAs) can be distinguished from contaminating labile nucleic acid structures. These data compare the electrophoretic mobility of hybrid PNA–DNA junctions vs. i) a classic immobile DNA 4WJ, J1 and ii) contaminating nucleic acid structures.
url http://www.sciencedirect.com/science/article/pii/S2352340915002619
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AT filberttotsingan supportingdataforthecharacterizationofpnadnafourwayjunctions
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