Human Origin Lactobacillus casei Isolated from Indonesian Infants Demonstrating Potential Characteristics as Probiotics in vitro

• Main Authors: Widodo ., Tiyas Tono Taufiq, Ety Aryati, Asih Kurniawati, Widya Asmara
• Format: Article
• Language: English
• Published: Universitas Gadjah Mada, Yogyakarta 2015-11-01
• Series: Indonesian Journal of Biotechnology
• Online Access: http://journal.ugm.ac.id/ijbiotech/article/view/7852

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mso-style-qformat:yes; mso-style-parent:""; mso-padding-alt:0cm 5.4pt 0cm 5.4pt; mso-para-margin:0cm; mso-para-margin-bottom:.0001pt; mso-pagination:widow-orphan; font-size:11.0pt; font-family:"Calibri","sans-serif"; mso-ascii-font-family:Calibri; mso-ascii-theme-font:minor-latin; mso-fareast-font-family:"Times New Roman"; mso-fareast-theme-font:minor-fareast; mso-hansi-font-family:Calibri; mso-hansi-theme-font:minor-latin; mso-bidi-font-family:"Times New Roman"; mso-bidi-theme-font:minor-bidi;} </style> <![endif]--> <p class="MsoNormal"><span style="font-size: 9.5pt; font-family: &quot;BookAntiqua&quot;,&quot;serif&quot;;" lang="EN-GB">The aim of this experiment was to isolate and identify Lactic Acid Bacteria (LAB) from infant faeces</span></p> <p class="MsoNormal"><span style="font-size: 9.5pt; font-family: &quot;BookAntiqua&quot;,&quot;serif&quot;;" lang="EN-GB">and subsequent evaluation of its potential probiotics. LAB was isolated from faeces of infants who consumed</span></p> <p class="MsoNormal"><span style="font-size: 9.5pt; font-family: &quot;BookAntiqua&quot;,&quot;serif&quot;;" lang="EN-GB">breast milk as the only source of diet on </span><em><span style="font-size: 9.5pt; font-family: &quot;BookAntiqua-Italic&quot;,&quot;serif&quot;;" lang="EN-GB">L-cysteine</span></em><span style="font-size: 9.5pt; font-family: &quot;BookAntiqua&quot;,&quot;serif&quot;;" lang="EN-GB">-supplemented MRS Agar, and incubated on 37</span><span style="font-size: 5.5pt; font-family: &quot;BookAntiqua&quot;,&quot;serif&quot;;" lang="EN-GB">o</span><span style="font-size: 9.5pt; font-family: &quot;BookAntiqua&quot;,&quot;serif&quot;;" lang="EN-GB">C for 48</span></p> <p class="MsoNormal"><span style="font-size: 9.5pt; font-family: &quot;BookAntiqua&quot;,&quot;serif&quot;;" lang="EN-GB">hours. Colonies grew on this media were then identifi ed based on morphological, physiological and molecular</span></p> <p class="MsoNormal"><span style="font-size: 9.5pt; font-family: &quot;BookAntiqua&quot;,&quot;serif&quot;;" lang="EN-GB">approaches. Morphological and physiological identifi cations based on Gram staining, shape, motility, spore</span></p> <p class="MsoNormal"><span style="font-size: 9.5pt; font-family: &quot;BookAntiqua&quot;,&quot;serif&quot;;" lang="EN-GB">formation, catalase, CO</span><span style="font-size: 5.5pt; font-family: &quot;BookAntiqua&quot;,&quot;serif&quot;;" lang="EN-GB">2 </span><span style="font-size: 9.5pt; font-family: &quot;BookAntiqua&quot;,&quot;serif&quot;;" lang="EN-GB">and NH</span><span style="font-size: 5.5pt; font-family: &quot;BookAntiqua&quot;,&quot;serif&quot;;" lang="EN-GB">3 </span><span style="font-size: 9.5pt; font-family: &quot;BookAntiqua&quot;,&quot;serif&quot;;" lang="EN-GB">production, and the ability to grow on temperature at 10</span><span style="font-size: 5.5pt; font-family: &quot;BookAntiqua&quot;,&quot;serif&quot;;" lang="EN-GB">o</span><span style="font-size: 9.5pt; font-family: &quot;BookAntiqua&quot;,&quot;serif&quot;;" lang="EN-GB">C and 45</span><span style="font-size: 5.5pt; font-family: &quot;BookAntiqua&quot;,&quot;serif&quot;;" lang="EN-GB">o</span><span style="font-size: 9.5pt; font-family: &quot;BookAntiqua&quot;,&quot;serif&quot;;" lang="EN-GB">C.</span></p> <p class="MsoNormal"><span style="font-size: 9.5pt; font-family: &quot;BookAntiqua&quot;,&quot;serif&quot;;" lang="EN-GB">Molecular identifi cation based on the amplifi cation of 16S rRNA gene. The potential application of selected</span></p> <p class="MsoNormal"><span style="font-size: 9.5pt; font-family: &quot;BookAntiqua&quot;,&quot;serif&quot;;" lang="EN-GB">isolates for probiotics was evaluated based on the ability to grow on media with low pH and the addition</span></p> <p class="MsoNormal"><span style="font-size: 9.5pt; font-family: &quot;BookAntiqua&quot;,&quot;serif&quot;;" lang="EN-GB">of 0.5% bile salts, the ability to inhibit the growth of pathogenic </span><em><span style="font-size: 9.5pt; font-family: &quot;BookAntiqua-Italic&quot;,&quot;serif&quot;;" lang="EN-GB">Bacillus cereus </span></em><span style="font-size: 9.5pt; font-family: &quot;BookAntiqua&quot;,&quot;serif&quot;;" lang="EN-GB">and </span><em><span style="font-size: 9.5pt; font-family: &quot;BookAntiqua-Italic&quot;,&quot;serif&quot;;" lang="EN-GB">Eschericia coli</span></em><span style="font-size: 9.5pt; font-family: &quot;BookAntiqua&quot;,&quot;serif&quot;;" lang="EN-GB">, and </span><em><span style="font-size: 9.5pt; font-family: &quot;BookAntiqua-Italic&quot;,&quot;serif&quot;;" lang="EN-GB">in vitro</span></em><span style="font-size: 9.5pt; font-family: &quot;BookAntiqua&quot;,&quot;serif&quot;;" lang="EN-GB">adherence ability. On the basis of morphological, physiological and molecular analysis of 16S rRNA gene, it</span><em><span style="font-size: 9.5pt; font-family: &quot;BookAntiqua-Italic&quot;,&quot;serif&quot;;" lang="EN-GB"> </span></em><span style="font-size: 9.5pt; font-family: &quot;BookAntiqua&quot;,&quot;serif&quot;;" lang="EN-GB">was concluded that the selected isolate 1AF was a strain of </span><em><span style="font-size: 9.5pt; font-family: &quot;BookAntiqua-Italic&quot;,&quot;serif&quot;;" lang="EN-GB">Lactobacillus casei</span></em><span style="font-size: 9.5pt; font-family: &quot;BookAntiqua&quot;,&quot;serif&quot;;" lang="EN-GB">. Evaluation of probiotic </span><em><span style="font-size: 9.5pt; font-family: &quot;BookAntiqua-Italic&quot;,&quot;serif&quot;;" lang="EN-GB">in vitro </span></em><span style="font-size: 9.5pt; font-family: &quot;BookAntiqua&quot;,&quot;serif&quot;;" lang="EN-GB">showed that 60.4% of cells were resistant to pH 2.0 for 90 minutes. Survival of isolate 1AF after growing at</span><em><span style="font-size: 9.5pt; font-family: &quot;BookAntiqua-Italic&quot;,&quot;serif&quot;;" lang="EN-GB"> </span></em><span style="font-size: 9.5pt; font-family: &quot;BookAntiqua&quot;,&quot;serif&quot;;" lang="EN-GB">0.5% bile salts was 70.8%. The selected isolate 1AF showed the ability to inhibit the growth of </span><em><span style="font-size: 9.5pt; font-family: &quot;BookAntiqua-Italic&quot;,&quot;serif&quot;;" lang="EN-GB">Eschericia coli </span></em><span style="font-size: 9.5pt; font-family: &quot;BookAntiqua&quot;,&quot;serif&quot;;" lang="EN-GB">and </span><em><span style="font-size: 9.5pt; font-family: &quot;BookAntiqua-Italic&quot;,&quot;serif&quot;;" lang="EN-GB">Bacillus cereus </span></em><span style="font-size: 9.5pt; font-family: &quot;BookAntiqua&quot;,&quot;serif&quot;;" lang="EN-GB">with inhibitory zone of 12.00&plusmn;1,00 and 15.33&plusmn;1.53 mm, respectively. </span><em><span style="font-size: 9.5pt; font-family: &quot;BookAntiqua-Italic&quot;,&quot;serif&quot;;" lang="EN-GB">In vitro </span></em><span style="font-size: 9.5pt; font-family: &quot;BookAntiqua&quot;,&quot;serif&quot;;" lang="EN-GB">study on the</span><em><span style="font-size: 9.5pt; font-family: &quot;BookAntiqua-Italic&quot;,&quot;serif&quot;;" lang="EN-GB"> </span></em><span style="font-size: 9.5pt; font-family: &quot;BookAntiqua&quot;,&quot;serif&quot;;" lang="EN-GB">adherence value of isolate to solid plate was found at 46.5%. It is concluded that </span><em><span style="font-size: 9.5pt; font-family: &quot;BookAntiqua-Italic&quot;,&quot;serif&quot;;" lang="EN-GB">Lactobacillus casei </span></em><span style="font-size: 9.5pt; font-family: &quot;BookAntiqua&quot;,&quot;serif&quot;;" lang="EN-GB">isolate 1AF</span><em><span style="font-size: 9.5pt; font-family: &quot;BookAntiqua-Italic&quot;,&quot;serif&quot;;" lang="EN-GB"> </span></em><span style="font-size: 9.5pt; font-family: &quot;BookAntiqua&quot;,&quot;serif&quot;;" lang="EN-GB">is a potential candidate as probiotics and subject to further </span><em><span style="font-size: 9.5pt; font-family: &quot;BookAntiqua-Italic&quot;,&quot;serif&quot;;" lang="EN-GB">in vivo </span></em><span style="font-size: 9.5pt; font-family: &quot;BookAntiqua&quot;,&quot;serif&quot;;" lang="EN-GB">evaluation.</span><em></em></p>