Characterization of 6 alpha-hydroxylation of taurochenodeoxycholic acid in pig liver.
The properties of the species-specific 6 alpha-hydroxylation of taurochenodeoxycholic acid were studied in subcellular fractions from pig liver. The hydroxylation was observed in microsomes but not in mitochondria. A partially purified cytochrome P-450 fraction in the presence of NADPH-cytochrome P-...
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| Language: | English |
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Elsevier
1988-10-01
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| Series: | Journal of Lipid Research |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S0022227520387848 |
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doaj-905c3b5f75d24664ac53cff80f158bbc2021-04-25T04:21:01ZengElsevierJournal of Lipid Research0022-22751988-10-01278807812Characterization of 6 alpha-hydroxylation of taurochenodeoxycholic acid in pig liver.H BoströmThe properties of the species-specific 6 alpha-hydroxylation of taurochenodeoxycholic acid were studied in subcellular fractions from pig liver. The hydroxylation was observed in microsomes but not in mitochondria. A partially purified cytochrome P-450 fraction in the presence of NADPH-cytochrome P-450 reductase, NADPH, and phospholipid catalyzed 6 alpha-hydroxylation of taurochenodeoxycholic acid at a 160-fold higher rate than the microsomes. This cytochrome P-450 fraction did not catalyze 6 alpha-hydroxylation of 5 beta-cholestane-3 alpha,7 alpha-diol or testosterone, nor did it catalyze 7 alpha-hydroxylation of cholesterol.http://www.sciencedirect.com/science/article/pii/S0022227520387848 |
| collection |
DOAJ |
| language |
English |
| format |
Article |
| sources |
DOAJ |
| author |
H Boström |
| spellingShingle |
H Boström Characterization of 6 alpha-hydroxylation of taurochenodeoxycholic acid in pig liver. Journal of Lipid Research |
| author_facet |
H Boström |
| author_sort |
H Boström |
| title |
Characterization of 6 alpha-hydroxylation of taurochenodeoxycholic acid in pig liver. |
| title_short |
Characterization of 6 alpha-hydroxylation of taurochenodeoxycholic acid in pig liver. |
| title_full |
Characterization of 6 alpha-hydroxylation of taurochenodeoxycholic acid in pig liver. |
| title_fullStr |
Characterization of 6 alpha-hydroxylation of taurochenodeoxycholic acid in pig liver. |
| title_full_unstemmed |
Characterization of 6 alpha-hydroxylation of taurochenodeoxycholic acid in pig liver. |
| title_sort |
characterization of 6 alpha-hydroxylation of taurochenodeoxycholic acid in pig liver. |
| publisher |
Elsevier |
| series |
Journal of Lipid Research |
| issn |
0022-2275 |
| publishDate |
1988-10-01 |
| description |
The properties of the species-specific 6 alpha-hydroxylation of taurochenodeoxycholic acid were studied in subcellular fractions from pig liver. The hydroxylation was observed in microsomes but not in mitochondria. A partially purified cytochrome P-450 fraction in the presence of NADPH-cytochrome P-450 reductase, NADPH, and phospholipid catalyzed 6 alpha-hydroxylation of taurochenodeoxycholic acid at a 160-fold higher rate than the microsomes. This cytochrome P-450 fraction did not catalyze 6 alpha-hydroxylation of 5 beta-cholestane-3 alpha,7 alpha-diol or testosterone, nor did it catalyze 7 alpha-hydroxylation of cholesterol. |
| url |
http://www.sciencedirect.com/science/article/pii/S0022227520387848 |
| work_keys_str_mv |
AT hbostrom characterizationof6alphahydroxylationoftaurochenodeoxycholicacidinpigliver |
| _version_ |
1721510348458033152 |