Screening and Selection of Hypoallergenic Buckwheat Species

Screening and Selection of Hypoallergenic Buckwheat Species

Both common buckwheat (Fagopyrum esculentum) flour and meal cause an allergy in sensitive patients, and if unnoticed, it can be fatal. It has become a potential occupational hazard for some mill workers. The development of hypoallergenic buckwheat would be more efficient if natural mutants for aller...

Full description

Bibliographic Details
Main Authors: Arun Nair, Taiji Adachi
Format: Article
Language:English
Published: Hindawi Limited 2002-01-01
Series:The Scientific World Journal
Online Access:http://dx.doi.org/10.1100/tsw.2002.157
id doaj-903d941792984858b6eb211cab4fb40b
record_format Article
spelling doaj-903d941792984858b6eb211cab4fb40b2020-11-25T00:49:55ZengHindawi LimitedThe Scientific World Journal1537-744X2002-01-01281882610.1100/tsw.2002.157Screening and Selection of Hypoallergenic Buckwheat SpeciesArun Nair0Taiji Adachi1Kiyomoto Bio Co. Ltd., 2-28-4 Sakura, Gakuen Kibanadai, Miyazaki 889-2154, JapanLab of Plant Genes and Physiology, College of Agriculture, Osaka Prefecture University, 1-1, Gakuen-Cho, Sakai, Osaka 599-8531, JapanBoth common buckwheat (Fagopyrum esculentum) flour and meal cause an allergy in sensitive patients, and if unnoticed, it can be fatal. It has become a potential occupational hazard for some mill workers. The development of hypoallergenic buckwheat would be more efficient if natural mutants for allergenic protein are detected. A screening and selection method was developed using SDS-PAGE coupled with PCR techniques. SDS-PAGE analysis of 14 different species of buckwheat revealed that F. lineare and F. urophyllum lack the 22-kDa major allergenic protein. PCR-based screening with specific primers for sequences encoding the allergenic protein was also effective in distinguishing the allergen-deficient species.http://dx.doi.org/10.1100/tsw.2002.157
collection DOAJ
language English
format Article
sources DOAJ
author Arun Nair
Taiji Adachi
spellingShingle Arun Nair
Taiji Adachi
Screening and Selection of Hypoallergenic Buckwheat Species
The Scientific World Journal
author_facet Arun Nair
Taiji Adachi
author_sort Arun Nair
title Screening and Selection of Hypoallergenic Buckwheat Species
title_short Screening and Selection of Hypoallergenic Buckwheat Species
title_full Screening and Selection of Hypoallergenic Buckwheat Species
title_fullStr Screening and Selection of Hypoallergenic Buckwheat Species
title_full_unstemmed Screening and Selection of Hypoallergenic Buckwheat Species
title_sort screening and selection of hypoallergenic buckwheat species
publisher Hindawi Limited
series The Scientific World Journal
issn 1537-744X
publishDate 2002-01-01
description Both common buckwheat (Fagopyrum esculentum) flour and meal cause an allergy in sensitive patients, and if unnoticed, it can be fatal. It has become a potential occupational hazard for some mill workers. The development of hypoallergenic buckwheat would be more efficient if natural mutants for allergenic protein are detected. A screening and selection method was developed using SDS-PAGE coupled with PCR techniques. SDS-PAGE analysis of 14 different species of buckwheat revealed that F. lineare and F. urophyllum lack the 22-kDa major allergenic protein. PCR-based screening with specific primers for sequences encoding the allergenic protein was also effective in distinguishing the allergen-deficient species.
url http://dx.doi.org/10.1100/tsw.2002.157
work_keys_str_mv AT arunnair screeningandselectionofhypoallergenicbuckwheatspecies
AT taijiadachi screeningandselectionofhypoallergenicbuckwheatspecies
_version_ 1725250373952733184

Similar Items