Vitamin E and Provide Protective Effects Against Liver Injury Induced by HgCl: Role of CHOP, GPR87, and mTOR Proteins
Background and Objective: Mercury is one of the most harmful heavy metals and its toxicity causes severe multi-organ dysfunction. This study was designed to explore novel molecular pathways involved in the hepatoprotective effect of vitamin E (Vit-E) and Lactobacillius plantarum (Lac-B) against merc...
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doaj-90130320f7054d3a85ca65cdd16051682021-04-27T22:03:59ZengSAGE PublishingDose-Response1559-32582021-04-011910.1177/15593258211011360Vitamin E and Provide Protective Effects Against Liver Injury Induced by HgCl: Role of CHOP, GPR87, and mTOR ProteinsAhlam Alhusaini0Shahad Alghilani1Waad Alhuqbani2Iman H. Hasan3 Pharmacology and Toxicology Department, Faculty of Pharmacy, King Saud University, Riyadh, Saudi Arabia Faculty of Pharmacy, King Saud University, Riyadh, Saudi Arabia Faculty of Pharmacy, King Saud University, Riyadh, Saudi Arabia Pharmacology and Toxicology Department, Faculty of Pharmacy, King Saud University, Riyadh, Saudi ArabiaBackground and Objective: Mercury is one of the most harmful heavy metals and its toxicity causes severe multi-organ dysfunction. This study was designed to explore novel molecular pathways involved in the hepatoprotective effect of vitamin E (Vit-E) and Lactobacillius plantarum (Lac-B) against mercury toxicity. Method: Acute hepatotoxicity was induced by administration of high dose of mercuric chloride (HgCl 2 ) in male rats, Vit-E or/and Lac-B were given along with HgCl 2 for 2 weeks. The effects of those antioxidants were studied focusing on their anti-apoptotic, anti-oxidative stress and anti-inflammatory eficacies. Histopathological examinations were also conducted. Results: The administration of HgCl 2 induced liver injury which manifested by elevation in serum ALT and AST. Liver MDA, caspase-3 and TNF-α levels were markedly increased; whereas, GSH level and SOD activity were declined. HgCl 2 significantly elevated the expressions of hepatic CHOP, GPR87, NF-κB and mTOR. Histopathological examination revealed massive hepatocyte degeneration following HgCl 2 administration. Treatment with Vit-E or/and Lac-B restored the normal levels of the previously mentioned parameters, as well as improved hepatic architecture. Conclusion: Vit-E and Lac-B provided protective effect against HgCl 2 -induced hepatotoxicity via reduction of oxidative stress and inflammation, and downregulation of CHOP, GPR87, NF-κB and mTOR proteins’ expressions.https://doi.org/10.1177/15593258211011360 |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Ahlam Alhusaini Shahad Alghilani Waad Alhuqbani Iman H. Hasan |
spellingShingle |
Ahlam Alhusaini Shahad Alghilani Waad Alhuqbani Iman H. Hasan Vitamin E and Provide Protective Effects Against Liver Injury Induced by HgCl: Role of CHOP, GPR87, and mTOR Proteins Dose-Response |
author_facet |
Ahlam Alhusaini Shahad Alghilani Waad Alhuqbani Iman H. Hasan |
author_sort |
Ahlam Alhusaini |
title |
Vitamin E and Provide Protective Effects Against Liver Injury Induced by HgCl: Role of CHOP, GPR87, and mTOR Proteins |
title_short |
Vitamin E and Provide Protective Effects Against Liver Injury Induced by HgCl: Role of CHOP, GPR87, and mTOR Proteins |
title_full |
Vitamin E and Provide Protective Effects Against Liver Injury Induced by HgCl: Role of CHOP, GPR87, and mTOR Proteins |
title_fullStr |
Vitamin E and Provide Protective Effects Against Liver Injury Induced by HgCl: Role of CHOP, GPR87, and mTOR Proteins |
title_full_unstemmed |
Vitamin E and Provide Protective Effects Against Liver Injury Induced by HgCl: Role of CHOP, GPR87, and mTOR Proteins |
title_sort |
vitamin e and provide protective effects against liver injury induced by hgcl: role of chop, gpr87, and mtor proteins |
publisher |
SAGE Publishing |
series |
Dose-Response |
issn |
1559-3258 |
publishDate |
2021-04-01 |
description |
Background and Objective: Mercury is one of the most harmful heavy metals and its toxicity causes severe multi-organ dysfunction. This study was designed to explore novel molecular pathways involved in the hepatoprotective effect of vitamin E (Vit-E) and Lactobacillius plantarum (Lac-B) against mercury toxicity. Method: Acute hepatotoxicity was induced by administration of high dose of mercuric chloride (HgCl 2 ) in male rats, Vit-E or/and Lac-B were given along with HgCl 2 for 2 weeks. The effects of those antioxidants were studied focusing on their anti-apoptotic, anti-oxidative stress and anti-inflammatory eficacies. Histopathological examinations were also conducted. Results: The administration of HgCl 2 induced liver injury which manifested by elevation in serum ALT and AST. Liver MDA, caspase-3 and TNF-α levels were markedly increased; whereas, GSH level and SOD activity were declined. HgCl 2 significantly elevated the expressions of hepatic CHOP, GPR87, NF-κB and mTOR. Histopathological examination revealed massive hepatocyte degeneration following HgCl 2 administration. Treatment with Vit-E or/and Lac-B restored the normal levels of the previously mentioned parameters, as well as improved hepatic architecture. Conclusion: Vit-E and Lac-B provided protective effect against HgCl 2 -induced hepatotoxicity via reduction of oxidative stress and inflammation, and downregulation of CHOP, GPR87, NF-κB and mTOR proteins’ expressions. |
url |
https://doi.org/10.1177/15593258211011360 |
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