Vitamin E and Provide Protective Effects Against Liver Injury Induced by HgCl: Role of CHOP, GPR87, and mTOR Proteins

Background and Objective: Mercury is one of the most harmful heavy metals and its toxicity causes severe multi-organ dysfunction. This study was designed to explore novel molecular pathways involved in the hepatoprotective effect of vitamin E (Vit-E) and Lactobacillius plantarum (Lac-B) against merc...

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Main Authors: Ahlam Alhusaini, Shahad Alghilani, Waad Alhuqbani, Iman H. Hasan
Format: Article
Language:English
Published: SAGE Publishing 2021-04-01
Series:Dose-Response
Online Access:https://doi.org/10.1177/15593258211011360
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spelling doaj-90130320f7054d3a85ca65cdd16051682021-04-27T22:03:59ZengSAGE PublishingDose-Response1559-32582021-04-011910.1177/15593258211011360Vitamin E and Provide Protective Effects Against Liver Injury Induced by HgCl: Role of CHOP, GPR87, and mTOR ProteinsAhlam Alhusaini0Shahad Alghilani1Waad Alhuqbani2Iman H. Hasan3 Pharmacology and Toxicology Department, Faculty of Pharmacy, King Saud University, Riyadh, Saudi Arabia Faculty of Pharmacy, King Saud University, Riyadh, Saudi Arabia Faculty of Pharmacy, King Saud University, Riyadh, Saudi Arabia Pharmacology and Toxicology Department, Faculty of Pharmacy, King Saud University, Riyadh, Saudi ArabiaBackground and Objective: Mercury is one of the most harmful heavy metals and its toxicity causes severe multi-organ dysfunction. This study was designed to explore novel molecular pathways involved in the hepatoprotective effect of vitamin E (Vit-E) and Lactobacillius plantarum (Lac-B) against mercury toxicity. Method: Acute hepatotoxicity was induced by administration of high dose of mercuric chloride (HgCl 2 ) in male rats, Vit-E or/and Lac-B were given along with HgCl 2 for 2 weeks. The effects of those antioxidants were studied focusing on their anti-apoptotic, anti-oxidative stress and anti-inflammatory eficacies. Histopathological examinations were also conducted. Results: The administration of HgCl 2 induced liver injury which manifested by elevation in serum ALT and AST. Liver MDA, caspase-3 and TNF-α levels were markedly increased; whereas, GSH level and SOD activity were declined. HgCl 2 significantly elevated the expressions of hepatic CHOP, GPR87, NF-κB and mTOR. Histopathological examination revealed massive hepatocyte degeneration following HgCl 2 administration. Treatment with Vit-E or/and Lac-B restored the normal levels of the previously mentioned parameters, as well as improved hepatic architecture. Conclusion: Vit-E and Lac-B provided protective effect against HgCl 2 -induced hepatotoxicity via reduction of oxidative stress and inflammation, and downregulation of CHOP, GPR87, NF-κB and mTOR proteins’ expressions.https://doi.org/10.1177/15593258211011360
collection DOAJ
language English
format Article
sources DOAJ
author Ahlam Alhusaini
Shahad Alghilani
Waad Alhuqbani
Iman H. Hasan
spellingShingle Ahlam Alhusaini
Shahad Alghilani
Waad Alhuqbani
Iman H. Hasan
Vitamin E and Provide Protective Effects Against Liver Injury Induced by HgCl: Role of CHOP, GPR87, and mTOR Proteins
Dose-Response
author_facet Ahlam Alhusaini
Shahad Alghilani
Waad Alhuqbani
Iman H. Hasan
author_sort Ahlam Alhusaini
title Vitamin E and Provide Protective Effects Against Liver Injury Induced by HgCl: Role of CHOP, GPR87, and mTOR Proteins
title_short Vitamin E and Provide Protective Effects Against Liver Injury Induced by HgCl: Role of CHOP, GPR87, and mTOR Proteins
title_full Vitamin E and Provide Protective Effects Against Liver Injury Induced by HgCl: Role of CHOP, GPR87, and mTOR Proteins
title_fullStr Vitamin E and Provide Protective Effects Against Liver Injury Induced by HgCl: Role of CHOP, GPR87, and mTOR Proteins
title_full_unstemmed Vitamin E and Provide Protective Effects Against Liver Injury Induced by HgCl: Role of CHOP, GPR87, and mTOR Proteins
title_sort vitamin e and provide protective effects against liver injury induced by hgcl: role of chop, gpr87, and mtor proteins
publisher SAGE Publishing
series Dose-Response
issn 1559-3258
publishDate 2021-04-01
description Background and Objective: Mercury is one of the most harmful heavy metals and its toxicity causes severe multi-organ dysfunction. This study was designed to explore novel molecular pathways involved in the hepatoprotective effect of vitamin E (Vit-E) and Lactobacillius plantarum (Lac-B) against mercury toxicity. Method: Acute hepatotoxicity was induced by administration of high dose of mercuric chloride (HgCl 2 ) in male rats, Vit-E or/and Lac-B were given along with HgCl 2 for 2 weeks. The effects of those antioxidants were studied focusing on their anti-apoptotic, anti-oxidative stress and anti-inflammatory eficacies. Histopathological examinations were also conducted. Results: The administration of HgCl 2 induced liver injury which manifested by elevation in serum ALT and AST. Liver MDA, caspase-3 and TNF-α levels were markedly increased; whereas, GSH level and SOD activity were declined. HgCl 2 significantly elevated the expressions of hepatic CHOP, GPR87, NF-κB and mTOR. Histopathological examination revealed massive hepatocyte degeneration following HgCl 2 administration. Treatment with Vit-E or/and Lac-B restored the normal levels of the previously mentioned parameters, as well as improved hepatic architecture. Conclusion: Vit-E and Lac-B provided protective effect against HgCl 2 -induced hepatotoxicity via reduction of oxidative stress and inflammation, and downregulation of CHOP, GPR87, NF-κB and mTOR proteins’ expressions.
url https://doi.org/10.1177/15593258211011360
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